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2.4 EXTERNAL PRESSURE MYOGRAPH CONNECTIONS
This section illustrates how to connect the perfusion circuit, the superfusion circuit, oxygen supply,
pressure supply and suction device on to the Pressure Myograph.
2.4.1 CONNECTING PERFUSION FLOW AND PURGING THE SYSTEM WITH BUFFER
The Pressure Interface controls the perfusion flow. Instructions on how to control the perfusion flow are
provided in chapter 3.4. To connect the Pressure Interface to the Pressure Myograph, use the silicone
tube (internal diameter 1mm) delivered with the Pressure Myograph System.
• Connect the “perfusion inlet” at P1 on the Pressure Myograph (see figure 2.2 and 2.3 for Pressure
Myograph - 114P and Pulsatile Pressure Myograph – 112PP, respectively) to the P1 outlet on the cap
of the Pressure Interface bottle (see figure 2.4).
• Connect the “perfusion outlet” at P2 on the Pressure Myograph (see figure 2.2 and 2.3 for Pressure
Myograph - 114P and Pulsatile Pressure Myograph – 112PP, respectively) to the P2 inlet on the
Pressure Interface (see figure 2.4).
Perfusion outlet P2
Superfusion outlet
Inlet pipe for Oxygen/
carbogen supply
Access hole for
temperature or pH
probe
Superfusion
inlet pipe
Access hole for
adding buffer and
drugs
Extra Perfusion
outlet
Extra Perfusion inlet
Extra Perfusion
inlet P1
Suction pipe to vacuum
Figure 2.1 Example of a complete set-up of Pressure Myograph System with DMT inverted Microscope
