biochrom HB Ultrospec 7500, User Manual

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Ultrospec 7500 User Manual • 41 56 2050 REV03 • 95
Counter Ion Pathlength Factor
Na 23.00
K 39.10
TEA 1 102.2
TEOA 2 149.19
Other* Custom
1
2
*
Triethylamine
Triethanolamine
Other is values defined within the application method parameters.
Molar Extinction Coefficient is calculated for the base sequence defined within the application method parameters, and is the
sum values of their nearest-neighbour pair values, specifically:
A C G T/U*
A 13.7 10.6 12.5 11.4
C 10.6 7.3 9.0 7.6
G 12.6 8.8 10.8 10.0
T/U* 11.7/12.3 8.1/8.6 9.5/10.0 8.4/9.8
* The nucleotide on the left of the forward-slash is the DNA sequence while the nucleotide on the right of the forward-
slash is the RNA sequence.
References:
Breslauer, K., Frank, R., Blocker, H. and Marky, L. (1986). Predicting DNA duplex stability from the base sequence. Proceedings of the
National Academy of Sciences, 83(11), pp.3746-3750.
SantaLucia, J. (1998). A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics. Proceedings of
the National Academy of Sciences, 95(4), pp.1460-1465.