biochrom HB Ultrospec 7500, User Manual

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Ultrospec 7500 User Manual • 41 56 2050 REV03 • 52
Protein
The Protein screen is accessed from the home screen. It contains predefined protein quantification methods and a protein dye application
for fluorescent labelling efficiency of protein probes, based on the absorbance, prior to their use in microarrays. All calculations applied
within the Protein applications are described in the Useful Calculation section.
Protein UV
The Protein UV application is accessed from the Protein screen. It can be used to perform Protein quantification measurements at 280 nm
and to assess sample quality using A260/A280 and A260/A230 absorbance (A) ratios.
Step 1
Set the mode; “Christian Warburg”, “BSA”, “IgG”, “Lysozyme”, “Molar
extinction”, “Mass extinction”, “E 1%”, or “Custom”.
Step 2
Set the pathlength; “10 mm”, “Quantimate 500”, or, “Quantimate 200”.
Step 3
Enter any dilution factor to be applied to the absorbance measurement.
Set the initial sample volume of a value of up to four significant figures.
Then set the amount of diluent added to the initial volume of a value of
up to four significant figures.
Implement the changes and return to the parameters screen by
selecting the confirm icon.
Step 4
Set the background, “On” or “Off”.
If Christian Warburg”, “BSA”, “IgG”, or “Lysozyme” mode is selected,
skip step 5 and go straight to step 6.