biochrom HB Ultrospec 7500, User Manual

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Ultrospec 7500 User Manual • 41 56 2050 REV03 • 67
If using a cell changer, skip steps 16 through 17 and go straight to step 18.
Step 16
Insert the reference sample then take a reference measurement using the
reference measurement icon.
The acquired reference sample baseline will be applied to all subsequent
sample measurements.
Step 17
Replace the previous sample with a test sample then take a sample
measurement using the confirm key.
The exclamation icon (
) appearing beside the purity ratios, indicates
that they are outside the ideal Warburg and Christian range.
Repeat for all samples.
Step 18
Return to the home screen using the exit icon in the options menu OR use the additional options to save, print, and, or load previous
measurements (see the Additional Options section).
RNA
The RNA application is selected from the home screen. It can be used to perform RNA quantification measurements at 260 nm and to assess
sample quality using A260/A280 and A260/A230 absorbance (A) ratios. All calculation applied within the RNA application are described in
the Useful Calculation section.
Step 1
Set the pathlength; “10 mm”, “Quantimate 500”, or, “Quantimate 200”.
Step 2
Enter any dilution factor to be applied to the absorbance measurement.
Set the initial sample volume of a value of up to four significant figures.
Then set the amount of diluent added to the initial volume of a value of
up to four significant figures.
Implement the changes and return to the parameters screen by selecting
the confirm icon.