biochrom HB Ultrospec 7500, User Manual, Page: 58 / 104

Ultrospec 7500 User Manual • 41 56 2050 REV03 • 58
Step 19
Run the standards by selecting the replicates icon.
Insert the reference sample then take a reference measurement using the
reference measurement icon.
The acquired reference sample baseline will be applied to all subsequent
standard sample measurements.
Step 20
Replace the reference sample with the first standard sample then take a
sample measurement using the sample measurement icon for each
replicate of that standard sample.
Step 21
Proceed to the next standard measurement screen using the
right/forward arrow.
Step 22
Replace the previous standard sample with the next standard sample then
take a sample measurement using the sample measurement icon for each
replicate of that standard sample.
Repeat for all remaining standard samples if applicable.
Step 23
Leave the replicates function using the left/backward arrow.
Step 24
If the source of the calibration is set to “Standards”, skip step 2 4 and go to
step 25.
If source of the calibration is set to “Manual”. Define each standards
absorbance value by selecting the appropriate text box and entering a
value between -0.3 and 3.0 A.