LB 925 Crocodile miniWorkstation
6. Performance and Quality Control
84024BA2 Rev. 03, 04/2020
Page 43 of 68
6.
Performance and Quality Control
6.1
Performance Checks
Performance checks are necessary for every part of the
miniWorkstation and particular instrument functions in defined time
intervals. Please view the chapter on Software before programming
the respective protocols with the Crocodile Control Software. It is
recommended to use the same set of protocols every time the
performance checks are carried out. In cases of failure repeat the
checks and see the troubleshooting list.
6.1.1
Washer
NOTICE
The washer manifold provides 2 functions, a dispenser and a suction
function. Check both functions separately. For these checks, it is
necessary to determine the instrument specific optimized depth for
your specific microplate for dispensing and aspiration prior to the
check.
Use the Crocodile Control Software and set up a protocol with the
following settings:
1.
Prime washer inlet 1 with distilled water: 8 cycles; volume:
1000µl. Total priming volume for the inlet will be 64ml.
2.
Program a washer dispense step that dispenses 200µl of
distilled water to row 1-4 of a microplate, using inlet 1.
3.
Repeat the priming and the dispense step for inlet 2 and 3.
Inlet 2 is used for dispense in row 5-8 and inlet 3 for row 9-12.
4.
Program a manual step, followed by an aspiration step with
the following settings for the whole microplate.
Aspirate only / 1-2 cycles
Enable sweep mode: 4-5mm diameter; 2-3mm/s speed
5.
Perform the protocol with an empty microplate. Use the
manual step to inspect the fluid volumes to be equal in all
wells visibly. The residual volume after aspiration depends on
your individual settings and shall be approximately equal.
