LB 925 Crocodile miniWorkstation
8. Troubleshooting
84024BA2 Rev. 03, 04/2020
Page 57 of 68
from 10-30 minutes.
Substrate or conjugate
is no longer active or is
weak
Test conjugate and substrate for activity.
Enzyme inhibitor
present
Sodium azide will inhibit peroxidase reactions.
Plate reader settings
not optimal
Verify the wavelength and filter settings in the plate
reader.
Incorrect assay
temperature (too cold)
Use recommended incubation temperature.
Bring substrates and micro titer plate to room
temperature before use.
Excessive kit stress has
occurred
Check the record to see how many times the kit has
cycled from the refrigerator.
Check to see if the kit was left out too long or at
extreme temperatures
Inadequate volume of
substrate
Check that correct volume is dispensed
Check if the dispenser tubes are filled with reagent.
Check if there was enough priming.
Check for leakages in the dispense path (air bubbles
in the tubing: tubing is not correctly tightened)
Check if the dispense path is blocked: flush the
dispense path.
Clean the needle with the cleaning wire, if needed.
Assay plates were
compromised or
previously used
Be sure to refrigerate plates in sealed bags with a
desiccant to maintain stability.
Prevent condensation from forming on plates by
allowing them to equilibrate to room temperature
while in packing.
If partial plates are used, be sure to label used wells
to prevent reuse. Cover them with sealing tape and
use the remaining wells as soon as possible.
Do not share partially used plates with other plates.
Include a desiccant in the storage box.
Blocking protein
included in the coating
solution
Omit blocking protein from coating solution.
Dispenser lines were
not cleaned before use
Clean dispense path
Microbiological
contamination of the
washer system
Clean and decontaminate the washer system
according to the description in the chapter
Maintenance. If necessary change the tubing.
