LB 925 Crocodile miniWorkstation
5. Software
84024BA2 Rev. 03, 04/2020
Page 37 of 68
1.
Select a [Wash Process] and the number of [Cycles] for this
process. The list contains self-declaring processes for priming,
dispensing, aspiration or clearance of the system.
Additionally, three wash processes combining Aspiration (Asp)
and Dispense (Disp) are available.
The following sequences will be performed for 1 Cycle:
Standard:
For every selected column: Asp / Disp / final cycle: Asp
Overflow wash:
For every selected column: Asp and Disp simultaneously
Soak wash:
Disp all columns / Asp all columns
2.
Select an [Inlet] and optimize [Dispense Settings] and
[Aspiration Settings] according to your needs.
3.
Take special care in optimizing the <Depth> for the aspiration
of your specific microplate.
The default setting of the Manifold Depth for the Dispense
step usually needs no adjustment, but the default setting of
the Plate Adjustment for the Dispense step needs to be
optimized, to define the optimal horizontal position for the
dispensing of wash buffer.
The [Manifold Depth] and the [Plate Adjustment] of the
[Aspirate Settings] differ for each microplate type due to their
different physical dimensions. For optimal washing results, the
settings must be defined for each microplate type on every
Crocodile instrument.
To check your settings click <Test Depth>. Lift the microplate
holder on its left side to check if there is sufficient space
between aspiration needle and microplate well bottom. For
minimal residual volume, the needle should not touch the
bottom and the distance between the needle and the bottom
should be around 1-2 mm. If necessary, re-adjust.
Click <Apply> to save your entries.
4.
For good washing results it is recommended to enable the
[Sweep Mode] and optimize the settings for [Speed] and
[Diameter] for your plate.
5.
By default, the whole microplate is processed. If necessary,
select only the columns needed.
6.
Confirm your entries with <OK>.
