8. Troubleshooting
LB 925 Crocodile miniWorkstation
Page 56 of 68
84024BA2 Rev. 03, 04/2020
8.
Troubleshooting
In cases of malfunction or unexpected measurement values, see the
troubleshooting list and/or contact your local distributor or Berthold
Technologies.
IMPORTANT
The list contains troubleshooting items for Crocodile 5-in-one and 4-
in-one (without reader).
Problem
Possible
Cause
Solution
Dispenser is not working
Dirt in the pumping
system
Clean the dispenser daily according to the
description in chapter Maintenance.
Inefficient washing
Manifold is not parallel
to the microplate
Check the manifold is in the right position and
adjust the angle if needed.
Dispense or Aspiration
needle is blocked
Use the cleaning wire to get rid of the dirt in the
needles.
Rinse the washer afterwards.
Washing seems to be
not good enough
Optimize (increase) the height of the dispense step.
Use the soak wash program.
Increase the delay time in the wash settings.
Increase the number of wash cycles.
No signal or weak signal
Omission of key
reagent
Check that all reagents have been added in the
correct order
Incorrectly prepared,
incomplete or wrong
substrate
Make sure that the substrate selected is
appropriate for the enzyme conjugate (such as
pNPP for alkaline phosphatase and OPD or TMB for
peroxidase).
Make sure that fresh H
2
O
2
is added if necessary.
Washes too stringently
Reduce the number of Wash Cycles.
Reduce the volume of washing buffer. Choose
another wash protocol.
Eliminate or reduce detergent concentration in
washing buffer.
Incubation times
inadequate
Incubation times should be appropriate for the
system. Typical substrate development times vary
