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LB 925 Crocodile miniWorkstation
8. Troubleshooting
84024BA2 Rev. 03, 04/2020
Page 59 of 68
Uneven colour
development
Incomplete washing of
wells
Ensure that all wells are filled with wash buffer and
are aspirated completely.
Check the washer dispenser functionality: flush the
wash head, clean the dispense needles with the
cleaning wire if needed.
Check the washer aspiration functionality: Clean
aspiration needles with the cleaning wire, if needed.
Optimize the aspiration settings.
Unexpected or irregular
results
Wells not completely
aspirated
Completely aspirate wells between steps.
Check the washer aspiration functionality:
Clean aspiration needles with the cleaning wire, if
needed.
Optimize the aspiration settings.
Pipetting error, poor
dilution series
Check pipetting technique and double-check
calculations
Reagents poorly
mixed/ not at room
temperature before the
assay starts.
Be sure that reagents are thoroughly mixed and at
the right temperature before the Assay starts.
Poor or variable
adsorption of reagents
to plate
Check choice of coating buffer, usually PBS, pH 7.4
or carbonate-bicarbonate buffer, pH 9.6. Try
extending incubating time.
Consider different plates.
Check homogeneity of samples.
Omission of reagents
Be sure that reagents were prepared correctly and
added in the correct order.
Excessive time was
taken to add samples
and controls to the
assay plate
Be sure to have all materials set up and ready to use
quickly.
Dispense controls and samples onto the plate at the
same time.
Dilution error
Check pipetting technique and double-check
calculations.
Technique problem
Proper mixing of reagents and wash steps are
critical. Check respective steps.
System Errors, e.g. power failure. Check the system.