Beckman Coulter Avanti J-26S XPI Instructions For Use Manual Download Page 56

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PN B10093AE

2-20

Operation

Setting Up an Elutriation Run

NOTE

At the end of the run, after disassembling the liquid and power lines, be sure to replace the black

rubber stoppers in the ports at the left side of the centrifuge door. Failure to seal these openings will

prevent proper operation of the FRS system, which will cause a diagnostic shut down during the

next run.

Delay Control Knob

DELAY

ELUTRIATOR

Summary of Contents for Avanti J-26S XPI

Page 1: ...PN B10093AE April 2019 Instructions For Use Avanti J 26S XPI High Performance Centrifuge Beckman Coulter Inc 250 S Kraemer Blvd Brea CA 92821 U S A ...

Page 2: ...arks or registered trademarks of their respective holders Contact Us If you have any questions contact our Customer Support Center Worldwide find us via our website at www beckman com support technical In the USA and Canada call us at 1 800 369 0333 Outside of the USA and Canada contact your local Beckman Coulter Representative Find us on the World Wide Web at www beckman com Beckman Coulter Euroc...

Page 3: ...help for your instrument Issue AD 05 2016 Updates were made to the following sections Multi Compliance Label CHAPTER 1 Control Features Issue AE 04 2019 Updates were made to the following sections CHAPTER 1 Control Features Note Changes that are part of the most recent revision are indicated in text by a bar in the margin of the amended page ...

Page 4: ...PN B10093AE iv Revision History ...

Page 5: ...or comments that add value to the step or procedure being performed Following the advice in the Important adds benefit to the performance of a piece of equipment or to a process NOTE NOTE is used to call attention to notable information that should be followed during installation use or servicing of this equipment Safety During Installation and or Maintenance This instrument is designed to be inst...

Page 6: ...use a two wire extension cord or a two wire non grounding type of multiple outlet receptacle strip Do not place containers holding liquid on or near the chamber door If they spill liquid may get into the instrument and damage electrical or mechanical components Safety Against Risk of Fire This instrument is not designed for use with materials capable of developing flammable or explosive vapors Do ...

Page 7: ...materials of a higher group require more than one level of protection Dispose of all waste solutions according to appropriate environmental health and safety guidelines It is your responsibility to decontaminate the instrument and accessories before requesting service by Beckman Coulter Field Service RoHS Notice These labels and materials declaration table the Table of Hazardous Substance s Name a...

Page 8: ...e RoHS Notice Environmentally Friendly Use Period EFUP date Therefore it can safely be used indefinitely The circling arrows indicate the product is recyclable The date code on the label or product indicates the date of manufacture ...

Page 9: ...vironmental Label vii Introduction xvii Certification xvii Scope of Manual xvii Conventions xvii Typographic Conventions xviii CFC Free Centrifugation xviii Recycling Label xviii Multi Compliance Label xix CHAPTER 1 Description 1 1 Introduction 1 1 Centrifuge Function and Safety Features 1 1 Centrifuge Function 1 1 Safety Features 1 2 Centrifuge Chassis 1 2 Housing and Door 1 2 Rotor Chamber 1 3 D...

Page 10: ...reparation 2 3 Installing the Rotor 2 3 Manual Operation 2 4 Selecting a Rotor 2 4 Entering Run Speed 2 5 Entering RPM 2 5 Entering RCF 2 6 Entering Run Time 2 7 Entering a Timed Run 2 7 Entering a w2t Run 2 8 Entering a Continuous HOLD Run 2 9 Entering Run Temperature 2 10 Entering Acceleration and Deceleration Rates 2 11 Acceleration 2 11 Deceleration 2 12 Starting a Run 2 12 Changing Parameters...

Page 11: ...allation Requirements A 1 Preinstallation Requirements A 1 Electrical Requirements A 1 Single and Three Phase Power Connections A 2 Additional Requirements for Three Phase Power Connections A 4 Space and Location Requirements A 4 Securing the Centrifuge to the Floor A 5 Bio Safety Level 3 Installation A 5 Non Invasive Centrifuge Restraint System for Vinyl Floors A 5 Using J2 Series Rotors in the A...

Page 12: ...xii Contents Avanti J Series Centrifuge Warranty Related Documents ...

Page 13: ... 3 1 Emergency Door Release Latch Access 3 5 3 2 Manual Door Release 3 6 3 3 Venting the Chamber Vacuum 3 7 3 4 Front Panel Retaining Strip 3 8 A 1 Single Phase Electrical Requirements A 3 A 2 Three Phase Y Electrical Requirements A 3 A 3 Correct and Incorrect Three Phase Power Configurations A 4 A 4 Rear View and Dimensions A 6 A 5 Checking the Rotor for Drive Pins A 7 ...

Page 14: ...xiv Illustrations ...

Page 15: ...xv Tables Tables 2 1 Acceleration and Deceleration Settings 2 11 3 1 Diagnostic Message Chart 3 2 A 1 Nominal Supply Voltage Ratings for the Avanti J 26S XPI A 2 A 2 Required Wire Connections A 3 ...

Page 16: ...xvi Tables ...

Page 17: ...specifications CHAPTER 2 Operation summarizes procedures for operating the centrifuge CHAPTER 3 Troubleshooting lists system diagnostic messages together with probable causes and user performed corrective actions CHAPTER 4 Care and Maintenance contains procedures for routine maintenance as well as a brief list of supplies and replacement parts APPENDIX A Preinstallation Requirements contains instr...

Page 18: ...l This symbol is required in accordance with the Waste Electrical and Electronic Equipment WEEE Directive of the European Union The presence of this marking on the product indicates 1 The device was put on the European market after August 13 2005 and 2 The device is not to be disposed via the municipal waste collection system of any member state of the European Union It is very important that cust...

Page 19: ...ng Label section in this document 169502 This label indicates recognition by a Nationally Recognized Testing Laboratory NRTL that the instrument has met the relevant product safety standards NOTE 169502 is applicable to North American models only A CE mark indicates that a product has been assessed before being placed on the market and has been found to meet European Union safety health and or env...

Page 20: ...PN B10093AE xx Introduction Multi Compliance Label ...

Page 21: ...les such as mitochondria nuclei chloroplasts and crude microsomes Separation of blood cells and cellular components Pelleting of prokaryotic and eukaryotic cells Gradient separation for example Ficoll Hypaque and Percoll Nucleic acid precipitation Virus isolation Bacteriophase isolation The Avanti J 26S XPI centrifuge is microprocessor controlled providing interactive operation The instrument desi...

Page 22: ... spinning rotors and prevents run initiation unless the door is closed and locked The door locks when ENTER and START are pressed or when the POWER switch is turned off The exception to this is ZONAL mode in which open door operation up to 3000 RPM is allowed A steel casingsurrounds the rotor chambertoprovide operatorprotection in the unlikelyevent of a rotor mishap Dynamic Rotor Inertia Check DRI...

Page 23: ...ce chamberpressureto approximatelyone quarter atmosphere 190mm Hg Thepumpturnsonafter the run is started before rotor friction reaches a high level When the required vacuum level is reached the pump turns off Vacuum in the chamber is vented during rotor deceleration Temperature Sensing and Control The temperature control system is cooled by circulation of a non CFC based refrigerant The temperatur...

Page 24: ...gure 1 1 Figure 1 1 The Power Switch The power switch is a two position rocker switch I on O off which controls electrical power to the centrifuge The key switch has five positions indicating the five different operating modes NORMAL mode is used for regular closed door centrifugation ZONAL mode is used when the JCF Z continuous flow zonal rotor is installed In ZONAL mode the centrifuge can run up...

Page 25: ...lay 1 Actual Value Display 2 Setup Screen ROTOR ID Indicates the installed rotor At the start of a run this field is blank During acceleration the centrifuge identifies the installed rotor and displays the rotor name SPEED Indicates rotor speed in revolutions per minute RPM or in relative centrifugal field a term describing the ratio of the centrifugal acceleration at a specified radius and speed ...

Page 26: ... parameters of the last run performed Function Keys Pressing ENTER or another function key saves parameter entries TIME In time mode indicates the remaining run time in hours and minutes The TIME display begins counting down when START is pressed and continues counting down to 0 when deceleration begins In hold mode indicates the time elapsed since START was pressed After 99 hours and 59 minutes t...

Page 27: ... be set If more than 59 minutes are entered in the minutes field the system automatically converts the entry to hours and minutes Decelerating begins when the set time counts down to zero The corresponding 2t value can be displayed by pressing the W2T soft key 2t run An 2t value can be entered and the system will calculate the run time required to reach that value Valid 2t settings are different f...

Page 28: ...ressing A D once more moves the cursor to the Decel field Available acceleration rates are maximum slow and timed 1 to 10 minutes from 0 to 500 RPM Available deceleration rates are maximum slow timed 1 to 10 minutes from 500 to 0 RPM and no brake off OPT Pressed to put the centrifuge into program mode where you can enter run parameters into memory for later use Up to 30 step programs can be stored...

Page 29: ...ugal effect to 3 significant digits in exponential notation Temperature Setting range 10 to 40 C in 1 C increments Accuracy rotor temperature controlled to within 2 C of set temperature after equilibration a Ambient temperature range 16 to 38 C 60 to 100 F b Cooling fluid refrigerant R452A HFO Acceleration maximum slow or timed 1 to 10 min from 0 to 500 RPM Deceleration maximum slow timed 1 to 10 ...

Page 30: ...Clearances for adequate ventilation sides 7 6 cm 3 in back place air diverter against the wall 16 cm 6 25 in Surface Finish polyester control panel with polycarbonate coating overlay uncoated plastic on door and covers acrylic baking enamel on metal surfaces Electrical Requirements 200 208 240 V single phase instrument 180 264 VAC 30 A 50 60 Hz 230 V single phase instrument 180 264 VAC 30 A 50 Hz ...

Page 31: ...occasionally however a temporary conductivity caused by condensation must be expected Specification Description Rotor Profile Description Rotor Code Max RPMa Max RCF g Max Capacity Rotor Manual Number JA 30 50 Ti b Fixed Angle 34 8 place rmax 108 mm 30 50 26 000c 81 800 8 50 mL J TB 070 JA 25 50 Ti Fixed Angle 34 8 place d rmax 108 mm 25 50 25 000 75 600 8 50 mL J TB 056 JA 25 15 Ti Fixed Angle 25...

Page 32: ...Fixed Angle 24 place 45 adapter rmax 116 mm 25 adapter rmax 112 mm 18 1 18 000 17 000 42 100 36 300 24 1 8 mL 24 1 8 mL J TB 037 JA 18 Fixed Angle 23 10 place rmax 132 mm 18 18 000 47 900 10 100 mL J TB 035 JA 17 Fixed Angle 25 14 place rmax 132 mm 17 17 000 39 800 14 50 mL J TB 017 JLA 16 250 Fixed Angle 25 6 place rmax 134 mm 16 250 16 000 38 400 6 250 mL J TB 072 JA 14 Fixed Angle 25 6 place rm...

Page 33: ...0 6 500 mL J TB 006 JLA 10 500 Fixed Angle 20 6 place rmax 166 mm 10 500 10 000 18 600 6 500 mL J TB 048 F10BCI 6x500y Fixed Angle 23 6 place rmax 158 mm F500 previ ously 10 1 10 000 17 696 6 500 mL JLA 9 1000 Fixed Angle 20 4 place rmax 185 mm 9 1000 9 000 16 800 4 1000 mL J TB 073 JLA 8 1000 Fixed Angle 20 6 place rmax 222 8 mm 8 1000 8 000 15 970 6 1000 mL J TB 073 JS 13 1 Swinging Bucket 6 pla...

Page 34: ... 165 mm 7 5 7 500 10 400 4 250 mL J TB 007 JS 5 3 Swinging Bucket 4 place rmax 194 8 mm 5 3 5 300 6 130 24 microplates 8 deep well plates 4 square well plates J TB 089 JS 4 3 Swinging Bucket 4 place rmax 204 mm 4 3 4 300 4 220 4 750 mL J TB 050 JS 4 0 Swinging Bucket 4 place rmax 226 mm 4 0 4 000 4 050 4 1 liter 4 blood bags 12 microplates 148 RIA tubes J 6TB 006 Rotor Profile Description Rotor Co...

Page 35: ...at 26 000 RPM at 30 C ambient 4 C minimum at 21 500 RPM 30 C ambient c Maximum speed for the JA 30 50 Ti rotor is 26 000 RPM in the Avanti J 26 XPI centrifuge and 30 000 RPM in the Avanti J 30I centrifuge d Temperature performance for the JA 25 50 and JA 25 15 rotors in the Avanti J 26S XPI is as follows 10 C minimum at 25 000 RPM at 30 C ambient 4 C minimum at 21 500 RPM 30 C ambient e Temperatur...

Page 36: ...PN B10093AE 1 16 Description Available Rotors ...

Page 37: ...s according to good laboratory procedures and methods to prevent spread of disease Because spills may generate aerosols observe proper precautions for aerosol containment Do not run toxic pathogenic or radioactive materials in this centrifuge without taking appropriate safety precautions Biosafe containment should be used when Risk Group II materials as identified in the World Health Organization ...

Page 38: ... MM Hold or 2t then use the keypad to enter the run time or 2t value No entry is made in Hold mode 7 Press TEMP then use the keypad to enter the required run temperature 8 Press A D and press the MAX SLOW or TIME soft key Or skip this step and accept the default acceleration rate MAX 9 Press A D and press the MAX SLOW TIME or OFF soft key a Or skip this step and accept the default deceleration rat...

Page 39: ... for program step 1 or 2 to end or end the run by pressing STOP 7 When the rotor stops depress the foot pedal to open the chamber door Preparation Prepare the rotor for centrifugation as described in the rotor manual Installing the Rotor The power must be turned on before you can unlock and open the chamber door 1 Turn the power switch on I Indicator lights on the control panel light up 2 Step on ...

Page 40: ...h run Manual Operation When a function key ROTOR SPEED TIME TEMP A D or OPT is pressed the corresponding setup screen field blinks to indicate that a parameter can be entered or changed The field continues to blink until ENTER or another function key is pressed To change an entry before you ve pressed ENTER press CE and enter a different value To change an entry after you ve pressed ENTER press th...

Page 41: ... Entering Run Speed Enter a run speed up to the maximum speed of the rotor in use 26 000 RPM maximum Or enter a relative centrifugal field RCF value up to the maximum achievable RCF of the rotor Entering RPM 1 Press SPEED The Speed field flashes and RPM and RCF soft keys appear Rotor Speed 0 RPM 0 x G Time 00 00 Temp 25 C Max Temp 29 C Accel MAX Decel MAX 10 12 14 17 18 MORE SPEED TIME TEMP A D OP...

Page 42: ...essary and calculates and displays the g force applied to the rotor at that speed Entering RCF 1 Press SPEED RPM and RCF soft keys appear 2 Press the RCF soft key ROTOR TIME TEMP A D OPT ROTOR ID SPEED RPM RCF TIME HR MIN C TEMP Rotor JA 30 50 Speed RPM x G Time 00 00 Temp 25 C Max Temp 29 C Accel MAX Decel MAX RPM RCF RPM SPEED SPEED TIME TEMP A D OPT ROTOR Rotor JA 30 50 Speed 26 000 RPM x G Tim...

Page 43: ...n RCF value that is unachievable for the installed rotor a message will appear telling you the rotor s achievable RCF Entering Run Time Runtime can beenteredin hours and minutes or in 2t accumulatedcentrifugal effect Hold mode is used for continuous operation Entering a Timed Run 1 Press TIME HH MM W2T and HOLD soft keys appear 2 Use they keypad to enter the required time up to 99 hours 59 minutes...

Page 44: ...ed Deceleration begins when the TIME display has counted down to 0 During deceleration End flashes in the TIME display and the red indicator light on the STOP key flashes Entering a 2t Run 1 Press TIME HH MM W2T and HOLD soft keys appear 2 Press the W2T soft key to select the 2t mode The Time field flashes 3 Use the keypad to enter an 2t value The entry appears in the Time field The decimal point ...

Page 45: ...ntil the rotor stops spinning so set and actual 2t values may be different at the end of the run Entering a Continuous HOLD Run 1 Press TIME The HH MM W2T and HOLD soft keys appear 2 Press the HOLD soft key to select the hold mode HOLD appears in the Time field SPEED TIME TEMP A D OPT ROTOR ROTOR ID SPEED RPM RCF TIME w2t C TEMP Rotor JA 30 50 Speed 26 000 RPM 81 800 x G Time 1 05E03 w2t Temp 25 C...

Page 46: ...p field 3 Press the TEMP key again to move the cursor to the Max Temp field Entered temperature plus 4 C appears in the Max Temp field and YES and NO soft keys appear The default maximum temperature is the set temperature plus 4 C You can specify a different maximum temperature from 2 C above the set temperature to 44 C You can also specify whether or not you want the system to shut down if the ma...

Page 47: ... Maximum acceleration not available with the JS 24 38 and JS 24 15 rotors Full torque is used from 0 RPM until set speed is reached Slow acceleration Approximately 2 minutes from 0 to 500 pm Time depends on the mass of the rotor in use larger rotors take longer Above 500 RPM full torque is used until set speed is reached Timed acceleration Acceleration time from 0 to 500 RPM can be set from 1 to 1...

Page 48: ... your selection appears in the Decel field If you choose TIME enter a deceleration time from 1 to 10 minutes and press A D again or accept the 10 minute default setting Starting a Run 1 Press ENTER then START You must press START within 5 seconds of pressing ENTER The green light on the START key flashes and the rotor begins to spin SPEED TIME TEMP A D OPT ROTOR Rotor JA 30 50 Speed 26 000 RPM 81 ...

Page 49: ...ons and troubleshooting procedures Programmed Operation The centrifuge internal memory can store up to 30 programs each containing up to two steps each step is a set of run parameters Programs are retained in memory when the power is turned off Creating a New Program 1 Press OPT The Prog ram Recall field flashes and the message line says Enter program number 1 30 2 Press OPT again to move the curs...

Page 50: ...am that contains user entered settings in step 2 those values are retained until you change them a To check step 2 settings press the STEP2 soft key 5 To add a second step to program 1 press the STEP2 soft key 6 Enter parameters for step 2 a Press ENTER when done All parameters for step 1 and 2 are saved under the selected program number b To recheck the parameters in either step press the STEP1 o...

Page 51: ... field A message appears asking if you want to overwrite the program YES and NO soft keys appear 3 Press the YES soft key The step 1 parameters appear 4 To see step 2 parameters press the STEP2 soft key The step 2 parameters appear 5 Modify the parameters for either step a Press ENTER when done b Repeat for the other step if required The newly entered parameters appear 6 Press the SAVE soft key to...

Page 52: ...ding and unloading of the rotor while it is spinning The JCF Z continuous flow zonal rotor is the only rotor that can be used while the key switch is in the ZONAL position See the JCF Z rotor manual for complete rotor operating instructions SPEED TIME TEMP A D OPT ROTOR Accel 5 Decel 5 Prog Recall Setup 1 Step 1 STEP1 STEP2 SAVE EXIT Rotor JA 30 50 Speed 26 000 RPM 81 800 x G Time 00 20 Temp 20 C ...

Page 53: ...R then START The rotor begins accelerating to 2000 RPM which is the default loading speed If an 2t run is specified 2t starts accumulating To select a different loading speed enter a speed of 3000 RPM or less before or after the rotor starts to spin 5 Load the rotor ZONAL Accel MAX Decel MAX Rotor JCF Z Speed 0 RPM x G Time 00 00 Temp 25 C Max Temp 29 C JCF Z SPEED TIME TEMP A D OPT ROTOR Accel MA...

Page 54: ...ent unloading speed of 3000 RPM or below after the rotor reaches 2000 RPM 8 Open the door and unload the rotor DANGER Never try to slow or stop the rotor by hand 9 Press STOP to bring the rotor to a complete stop NOTE Before another zonal run can be started you must press the RESET key Setting Up an Elutriation Run Centrifuges equipped for elutriation have elutriation controls as shown in Figure 2...

Page 55: ...When the rotor reaches set speed turn on the strobe controls by pressing the strobe control power switch The power switch indicator lights 7 When the rotor speed has stabilized look through the port in the centrifuge door and turn the DELAY control knob until the elutriation chamber in the rotor is synchronized with the strobe The chamber will appear to be motionless Sample can now be injected int...

Page 56: ... the liquid and power lines be sure to replace the black rubber stoppers in the ports at the left side of the centrifuge door Failure to seal these openings will prevent proper operation of the FRS system which will cause a diagnostic shut down during the next run 1 Delay Control Knob DELAY ELUTRIATOR 1 ...

Page 57: ...operating conditions can be cleared by pressing CE and following the instructions in the message Diagnostic messages that result from abnormal operating conditions or equipment malfunction require troubleshooting Refer to Table 3 1 to determine the nature of the condition and any recommended actions If a problem persists after you have performed the recommended action call your Beckman Coulter Fie...

Page 58: ...ensitive electronics in the area If L2 continues to appear after cleaning call Beckman Coulter Field Service a L3 Door problem refer to manual L9 Door problem refer to manual Foot pedal was depressed before end of run Latches are not operating properly Press CE and try to restart the run If this does not work call Beckman Coulter Field Service a L4 Door problem refer to manual Door stays locked af...

Page 59: ...s CE If F2 message reappears perform same actions as for Fl error F3 FRS failure refer to manual FRS level too high Run shuts down with maximum brake Call Beckman Coulter Field Service a S1 System error There is a problem with the system control software EPROM or RAM System shuts down Call Beckman Coulter Field Service a H4 Speed error a run is in progress at speed or accelerating the operator cha...

Page 60: ...ent of an extended power failure you may need to override the door locking mechanism manually to remove the rotor and retrieve your sample I1 Imbalance error Rotor load is severely out of balance Run shuts down with maximum brake Make sure that tubes or bottles are loaded symmetrically in the rotor With swinging bucket rotors lubricate the buckets according to the rotor manual instructions Unlubri...

Page 61: ...receptacle The Mains power plug is the disconnect device and must remain easily accessible Position the centrifuge so that it is easy to remove the Mains power plug from the outlet receptacle 2 Insert a 4 mm or 5 32 in Allen wrench straight through one of the holes either one and turn the wrench to the left counterclockwise until the latch disengages 3 Repeat step 2 inserting the Allen wrench thro...

Page 62: ...ut a 45 degree angle and while holding it out step on the foot pedal Depending on the level of vacuum in the chamber at the time of the power failure the door may or may not open a If it opens go to step 6 b If it does not open you will need to manually vent the vacuum Go to step 7 6 After the door opens first release the foot pedal and then release the interlock lever Sample can be removed a Go t...

Page 63: ...u grasp the hose place your hand over these lines Make sure that they stay connected as you pull CAUTION Do not twist the hose to the left counterclockwise as you pull the hose or you will unscrew the hose fitting Figure 3 3 Venting the Chamber Vacuum 8 Replace the hose by pushing it down over the fitting as far as it can go 1 Vacuum Pump 2 Grasp red hose and pull up Do not disconnect the tubing l...

Page 64: ...Holding the panel at the top with the instrument label facing out insert the three grooved brackets on the bottom inside of the door over the front panel retaining strip see Figure 3 4 a Push the top of the front panel into place Figure 3 4 Front Panel Retaining Strip 2 Hold the left top edge in place and insert the Allen wrench through the hole a Turn to the right clockwise until the latch engage...

Page 65: ... rotor or in an older JCF Z rotor with newly replaced bearings run the rotor from 0 to 5000 RPM and back to 0 RPM three times This procedure will distribute lubricant around the bearings in the rotating seal assembly reducing drag 2 In older JCF Z rotors with bearings that have not been replaced recently follow the same procedure as for a new JCF Z rotor perform three runs from 0 to 5000 RPM and b...

Page 66: ...PN B10093AE 3 10 Troubleshooting JCF Z Rotor Identification ...

Page 67: ...pose parts which involve the risk of electric shock or personal injury Make sure that the power switch is off O and the centrifuge is disconnected from the main power source by removing the Mains power plug from the outlet receptacle and refer such servicing to qualified service personnel WARNING Do not use alcohol or other flammable substances in or near operating centrifuges Maintenance Perform ...

Page 68: ...orrosives or contaminants from drying on component surfaces 2 To prevent accumulations of sample dust and or glass particles from broken sample tubes keep the chamber clean and dry by frequent wiping with a cloth or paper towel a For thorough cleaning wash the chamber using a mild detergent such as Solution 555 b Dilute the detergent with water 10 parts water to 1 part detergent c Rinse thoroughly...

Page 69: ...hanol 70 may be used to clean any exterior surface of the centrifuge See Chemical Resistances publication IN 175 for chemical resistances of centrifuge and accessory materials For this centrifuge refer to the PET column in the chemical resistances chart for the plastic surfaces and the polyurethane paint column for the painted metal surfaces CAUTION Ethanol is a flammability hazard Do not use it i...

Page 70: ...rifuge b Holding the filter by the side edges insert the top half behind the frame edge and lift up until the bottom half clears the retaining strip c Then set the bottom edge down Circuit Breaker and Fuses There are no user replaceable fuses in the Avanti J 26S XPI centrifuge If the centrifuge circuit breaker trips for any reason the power switch will move to the OFF O position 1 Reset the circui...

Page 71: ...ree from pathogens and or radioactivity Sterilization and decontamination must be done before returning the parts All parts must be accompanied by a note plainly visible on the outside of the box or bag stating that they are safe to handle and that they are not contaminated with pathogens or radioactivity Failure to attach this notification will result in return or disposal of the items without re...

Page 72: ...steel M4 threads 12 mm qty 2 Cable Clamp nylon 6 4 mm 1 4 in I D qty 10 366431 366430 893412 893411 000499 Zonal Tubing Adapter for Cole Parmer size 14 tubing 4 8 mm 3 16 in O D Note kit 366431 is also required Tubing Adapter stainless steel for size 14 tubing qty 2 Cable Clamp nylon 4 8 mm 3 16 in I D qty 10 363844 363830 003343 Zonal Tubing Adapter for Cole Parmer size 15 tubing 9 6 mm 3 8 in O ...

Page 73: ...it breakers Power receptacle see Figure A 1 or Figure A 2 Drill for drilling holes in the floor for installation of anchoring kit bolts see Securing the Centrifuge to the Floor later in this section A 9 5 mm 3 8 inch drill is required for concrete floors A 6 4 mm 1 4 inch drill is required for wood floors Electrical Requirements Power to the centrifuge should originate directly from a main power l...

Page 74: ...hows the power connection for single phase centrifuges including earth ground and two power leads with 30 ampere circuit breakers Figure A 2 shows the power connection for three phase centrifuges including earth ground and three power leads with minimum 16 ampere circuit breakers and common neutral connection Table A 2 contains wiring information Table A 1 Nominal Supply Voltage Ratings for the Av...

Page 75: ...30 Bryant 96 30 FR or Equivalent NEMA 6 30 R 3 Earth Ground 4 Measured Line Voltage 1 16 ampere Circuit Breaker 2 Neutral 3 Measured Line Voltage Phase to Phase 4 Earth Ground Table A 2 Required Wire Connections Wire Insulation Color Terminal Symbol Harmonized North American Green Yellow Earth ground Light Blue Neutral N L Brown Live or Line L L Black three phase connections only Live or Line qty ...

Page 76: ...he wire gauge required at a particular site must be determined by a facilities engineer at that site Figure A 3 Correct and Incorrect Three Phase Power Configurations Space and Location Requirements 1 Locate the instrument on a clean level floor The Mains power plug is the disconnect device and must remain easily accessible Position the centrifuge so that it is easy to remove the Mains power plug ...

Page 77: ...entrifuge from moving in the unlikely event of a rotor mishap Complete instructions for installing the anchoring kit are packaged with the hardware which is shipped with the centrifuge The instructions publication B10122 include a full size template to be used as a guide for drilling holes in the floor Refer to this document for additional installation instructions NOTE Beckman Coulter representat...

Page 78: ...tallation Requirements Preinstallation Requirements Figure A 4 Rear View and Dimensions 1 Air Exit 2 Power Cable 3 Air Diverter 4 Depth 5 Air Intake 1 5 4 3 2 1 8 m 6 0 ft 16 0 6 25 71 28 86 34 149 58 5 116 45 5 86 34 cm in ...

Page 79: ...ns in the rotor drive hole see Figure A 5 These drive pins engage with the centrifuge spindle hub to ensure that the rotor does not slip during acceleration Some Beckman Coulter rotors including the JA 10 the JS 7 5 the JA 14 and the JS 13 have been manufactured without drive pins because pins were not needed when these rotors were used in J2 series centrifuges Figure A 5 Checking the Rotor for Dr...

Page 80: ... in the Zonal Bracket Kit part number 366431 The Zonal Bracket Kit includes a bracket mounting hardware and assembly instructions This kit is for use with Cole Parmer tubing size 16 6 4 mm 1 4 in O D the tubing size most commonly used with the JCF Z rotor Two additional kits are available one which contains adapters for size 14 tubing 4 8 mm 3 16 in O D and one which contains adapters for size 15 ...

Page 81: ...lace the bearings See the JCF Z rotor instruction manual publication JCFZ IM for bearing replacement instructions Be sure to follow instructions in the next paragraph below for wearing in the bearings On the first few uses of a new JCF Z rotor or when the bearings have been replaced in an older JCF Z rotor lubrication has not yet been thoroughly distributed around the bearings in the bearing housi...

Page 82: ...PN B10093AE A 10 Preinstallation Requirements Using J2 Series Rotors in the Avanti J 26S XPI ...

Page 83: ...perature precool or prewarm the rotor and test samples to the required temperature The length of precooling prewarming time depends on how different the rotor starting temperature is from the required run temperature 2 Install the rotor with adapters if applicable and tubes or bottles filled with sample buffer or water if above 2 C 3 Set the speed to 2000 RPM the time to HOLD and the temperature t...

Page 84: ...N B10093AE B 2 Temperature Calibration Procedure Introduction For example If the required sample temperature is And the measured buffer water temperature is Set the temperature to 4 C 6 C 2 C 5 C 4 C 6 C ...

Page 85: ...aid and will be returned to Buyer with the transportation charges collect unless the product is found to be defective in which case Beckman Coulter will pay all transportation charges Beckman Coulter makes no warranty concerning products or accessories not manufactured by it In the event of failure of any such product or accessory Beckman Coulter will give reasonable assistance to the Buyer in obt...

Page 86: ...PN B10093AE Warranty 2 Beckman Coulter Inc Avanti J Series Centrifuge Warranty ...

Page 87: ......

Page 88: ...ories Using Fixed Angle Rotors Using Swinging Bucket Rotors Using Vertical Tube and Rack Type Rotors Care and Maintenance Chemical Resistances Temperature Compensation Tables Gradient Materials Blood Component Separation Chemical Resistances for Beckman Coulter Centrifugation Products PN IN 175M The JE 5 0 Elutriation System JA 18 Fixed Angle Rotor PN J TB 035 High Performance and High Capacity Ce...

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