Bayer HealthCare ADVIA 60, Operator'S Manual

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4. TECHNOLOGY
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2 - Flag
M2
: Located in the 130 to 160 fL zone, this flag informs the lab operator of the presence
of lymphoblasts, myelocytes, abnormal lymphocytes, or basophilia (too many basophils). This
flag can be adjusted by the operator. It corresponds to the number of cells counted by the detection
zone out of the total number of granulocytes. The limit which triggers this flag is adjusted by the
lab operator.
3 - Flag
G1
: Situated in the 160 to 220 fL zone, this detects the presence of eosinophils,
myelocytes, and sometimes, neutrophilia. This flag can be adjusted by the operator. It corres-
ponds to the number of cells counted in the detection area over the total number of granulocytes.
The limit which triggers this flag is adjusted by the lab operator.
4 - Flag
G2
: This flag makes it possible to follow an abnormal granulocyte peak displacement,
shows anomalies in the membrane of the granulocytes, and also possible lyse or hydraulic
anomalies. This flag is also triggered, if the blood is too old (after 6 - 8 hours). The flag is also
triggered if the granulocyte volume is less than 250 fL .
5 - Flag
G3
: This flag is situated in the zone which is greater than 400 fL. It detects the presence
of metamyelocytes. The flag is set off when the percentage in number, compared to the number
of granulocytes is higher than the level set. The lab operator can adjust the limit which triggers
this flag.
4.3.4. Comments on the flagging capabilities
All anomalies and/or abnormal distributions signaled by the ADVIA 60-
CT
should be verified
manually for the presence of pathological elements. As a result of the differential resistance of
cytoplasmic membranes in the different cell types, pathological elements can be found in a
number of different zones. This also applies to the presence of normal or non-pathological cells
that have been subject to chemotherapy or some other form of treatment in alarm zones. This will
result in a false alarm.