Cycle/Step
Stage
Reaction
volume
(µL)
Cover
temp. (°C)
1
2
3
4
Step 3
RampRate
50
105.0
—
6.0
—
—
Temperature
72.0
Hold Time
30
Touchdown PCR Method
Cycle/Step
Stage
Reaction
volume
(µL)
Cover
temp. (°C)
1
2
3
4
Cycles
20
10
—
—
50
105.0
Step 1
RampRate
6.0
6.0
Temperature
94.0
94.0
Hold Time
15
15
Step 2
RampRate
6.0
6.0
Temperature
65.0
55.0
Hold Time
30
30
AutoDelta
Temperature
-0.5
—
AutoDelta
Starting
Cycle
1
—
3D
Cycle/Step
Stage
Reaction
volume
(nL)
Cover
temp. (°C)
1
2
3
4
Cycles
1x
39x
1x
—
1
70.0
Step 1
RampRate
0.8
1.2
1.2
Temperature
96.0
60.0
60.0
Hold Time
600
120
120
Step 2
RampRate
—
0.8
1.2
Temperature
—
98.0
10.0
Hold Time
—
30
∞
Appendix E
Predefined run methods
Location of power point and ports on the instrument
E
80
SimpliAmp
™
Thermal Cycler User Guide
Summary of Contents for SimpliAmp Thermal Cycler
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