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Agilent InfinityLab LC Series Vialsamplers User Manual

315

Appendix

Refrigerant

The refrigerant HFC-134a is used only in the Agilent Infinity II Sample Cooler.

Table 29

Physical properties of refrigerant HFC-134a

Molecular weight

102

Critical temperature

101.1 °C

Critical pressure

40.6 bar

Boiling point

-26.5 °C

Table 30

Physical properties of refrigerant R600a (isobutane)

Molecular weight

58.12

Critical temperature

134.98 °C

Critical pressure

36.6 bar

Boiling point

-11.7 °C

Summary of Contents for 1260 Infinity II Series

Page 1: ...Agilent Technologies Agilent InfinityLab LC Series Vialsamplers User Manual ...

Page 2: ...scribed in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or sub contract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR ...

Page 3: ... chapter provides information on environmental requirements physical and performance specifications 3 Using the Module This chapter provides information on how to use the module 4 Installation of the Optional Sample Cooler or Thermostat This chapter describes the installation of the optional sample cooler or thermostat 5 Preparing the Module This chapter explains the operational parameters of the ...

Page 4: ...ir of the module 10 Parts and Materials for Maintenance This chapter provides information on parts for maintenance 11 Identifying Cables This chapter provides information on cables used with the Agilent 1200 Infinity Series modules 12 Hardware Information This chapter describes the detector in more detail on hardware and electronics 13 LAN Configuration This chapter provides information on connect...

Page 5: ...ical Specifications 40 Performance Specifications 41 Specifications of the Sample Cooler 47 Specifications of the Sample Thermostat 49 Specifications of the Integrated Column Compartment 51 3 Using the Module 53 Magnets 54 Turn on off 55 Status Indicators 57 Exchange Drawers 58 Install the Integrated Column Compartment 60 Install the Column Shelf 66 Install the External Tray 70 Install a Column IC...

Page 6: ...7 Setting up the Vialsampler 119 6 Optimizing Performance 131 Optimization for Lowest Carry over 132 Fast Injection Cycle and Low Delay Volume 136 Precise Injection Volume 138 Choice of Rotor Seal 140 7 Troubleshooting and Diagnostics 141 Overview of the Module s Indicators and Test Functions 142 Maintenance Functions 144 Step Commands 153 Tests 155 Troubleshooting 156 Troubleshooting Guide for th...

Page 7: ... the Gripper Arm 216 Replace the Peristaltic Pump Cartridge 219 Exchange the Wash Port Assembly 222 Replace the Module Firmware 228 10 Parts and Materials for Maintenance 229 Main Assemblies 230 Standard Parts 232 Drawer Assembly 233 External Tray 235 Analytical Head Assembly 40 μL 236 Analytical Head Assembly 100 µL 237 2ps 6pt Injection Valve 1300 bar 238 2ps 6pt Injection Valve 800 bar 239 2ps ...

Page 8: ...rument Layout 281 Early Maintenance Feedback EMF 282 13 LAN Configuration 283 What You Have to Do First 284 TCP IP parameter configuration 285 Configuration Switches 286 Initialization Mode Selection 287 Dynamic Host Configuration Protocol DHCP 289 Manual Configuration 292 PC and Agilent ChemStation Setup 297 14 Appendix 307 General Safety Information 308 Waste Electrical and Electronic Equipment ...

Page 9: ...on G7129B 12 Features G7129B 13 Product Description G7129C 14 Features G7129C 15 Overview of the Module 16 Operating Principle 18 Sampling Sequence 18 Needle Parkstation 23 Hydraulic Box 24 Transport Assembly 26 Leak and Waste Handling 28 Leak Sensor 32 Waste Guidance 32 Waste Concept 33 This chapter gives an introduction to the module and instrument overview ...

Page 10: ...ine pharmaceutical tasks and quality control as well as for environmental and food analyses It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 C as well as a sample cooler sample thermostat for stable temperatures down to 4 C or from 4 C up to 40 C all within one module Figure 1 Overview of the Vialsampler 6WDWXV LQGLFDWRU 1HHGOH DVVHPEO ...

Page 11: ...mns up to 30 cm length and providing heating capacity up to 80 C for reproducible chromatography data at optimized resolution Integrated sample cooler as option or upgrade available providing high cooling capacity for all vials loaded down to 4 C Integrated sample thermostat as option or upgrade available providing high cooling and heating capacity for all vials loaded from 4 C to 40 C Low interna...

Page 12: ... of use needed for routine pharmaceutical tasks and quality control as well as for environmental and food analyses It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 C as well as a sample cooler sample thermostat for stable temperatures down to 4 C or from 4 C up to 40 C all within one module Figure 2 Overview of the Vialsampler 6WDWXV LQ...

Page 13: ... up to 30 cm length and providing heating capacity up to 80 C for reproducible chromatography data at optimized resolution Integrated sample cooler as option or upgrade available providing high cooling capacity for all vials loaded down to 4 C Integrated sample thermostat as option or upgrade available providing high cooling and heating capacity for all vials loaded from 4 C to 40 C Low internal v...

Page 14: ...ine pharmaceutical tasks and quality control as well as for environmental and food analyses It can house optionally the integrated column compartment for two LC columns with temperature control up to 80 C as well as a sample cooler sample thermostat for stable temperatures down to 4 C or from 4 C up to 40 C all within one module Figure 3 Overview of the Vialsampler 6WDWXV LQGLFDWRU 1HHGOH DVVHPEO ...

Page 15: ...mns up to 30 cm length and providing heating capacity up to 80 C for reproducible chromatography data at optimized resolution Integrated sample cooler as option or upgrade available providing high cooling capacity for all vials loaded down to 4 C Integrated sample thermostat as option or upgrade available providing high cooling and heating capacity for all vials loaded from 4 C to 40 C Low interna...

Page 16: ...d up by the gripper arm and positioned below the needle station The gripper transport mechanism the needle station and the hydraulic unit are driven by motors Movement is monitored by optical sensors and optical encoders to ensure correct operation The metering device is always flushed after injection to ensure minimum carry over The standard analytical head of the metering device provides injecti...

Page 17: ...ined with the Agilent 1290 Infinity Sample Cooler G7167 60005 or with a Sample Thermostat G7167 60101 The combination of the Vialsampler with the sample cooler is called a cooled Vialsampler the combination of the Vialsampler with the sample thermostat is called a thermostated Vialsampler For more details refer to the 1290 Infinity Sample Cooler or Sample Thermostat documentation ...

Page 18: ... when the injection valve returns to the mainpass main path position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The piston of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts out of the sea...

Page 19: ...ath position Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass main path position see Figure 4 on page 19 In this position the mobile phase flows through the autosamplers metering device sample loop and needle This ensures that all parts in contact with sample are flushed during the run thus minimizing carry over Figure 4 Ma...

Page 20: ... the valve unit switches to the bypass position see Figure 5 on page 20 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figure 5 Valve in bypass needle in vial metering device aspirates sample volume 9LDO DVK SRUW 1HHGOH VHDW 6DPSOLQJ ORRS 6ROYHQW DVK SXPS 0HWHULQJ GHYLFH ...

Page 21: ...the needle The needle moves down into the vial the metering unit draws the required sample volume into the loop and the needle is raised In the next step the needle is washed see Figure 6 on page 21 Figure 6 Outer face of needle getting washed in wash port 9LDO DVK SRUW 1HHGOH VHDW 6DPSOLQJ ORRS 6ROYHQW DVK SXPS 0HWHULQJ GHYLFH ...

Page 22: ... in the sample tray The wash port flips into the origin position the needle is lowered into the needle seat and the injection valve switches back to mainpass main path position flushing the sample onto the column see Figure 7 on page 22 Figure 7 Valve switches to mainpass main path sample is transferred towards the LC column 9LDO DVK SRUW 1HHGOH VHDW 6DPSOLQJ ORRS 6ROYHQW DVK SXPS 0HWHULQJ GHYLFH ...

Page 23: ...Drive The needle movement is driven by a stepper motor connected to the spindle assembly by a toothed belt The circular motion of the motor is converted to linear motion by the drive nut on the spindle assembly The upper and lower needle positions are detected by reflection sensors on the needle station board while the needle in vial position is determined by counting the motor steps from the uppe...

Page 24: ...ive nut pushes the sapphire piston against the tension of the spring into the analytical head The base of the piston sits on the large bearing of the drive nut which ensures the piston is always centered A ceramic ring guides the movement of the piston in the analytical head The home position of the piston is sensed by an optical sensor on the hydraulic unit board while the sample volume is determ...

Page 25: ...valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass main path end positions No valve adjustments are required after replacing internal components ...

Page 26: ...losed loop mode for accurate positioning of the gripper assembly The rotational movement of the motors is converted to linear motion X and Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a ...

Page 27: ...ally and correct for position errors automatically e g if the gripper is accidentally moved out of position when loading vials into the vial tray The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition ar...

Page 28: ...l leak plane outlets are situated in a consistent position so that all Infinity and Infinity II modules can be stacked on top of each other Waste tubes are guided through a channel on the right hand side of the instrument keeping the front access clear from tubes The leak plane provides leak management by catching all internal liquid leaks guiding them to the leak sensor for leak detection and pas...

Page 29: ...Agilent InfinityLab LC Series Vialsamplers User Manual 29 Introduction 1 Leak and Waste Handling Figure 11 Infinity II Leak Waste Concept Flex Bench installation ...

Page 30: ...30 Agilent InfinityLab LC Series Vialsamplers User Manual 1 Introduction Leak and Waste Handling Figure 12 Infinity II Single Stack Leak Waste Concept bench installation ...

Page 31: ...Manual 31 Introduction 1 Leak and Waste Handling Figure 13 Infinity II Two Stack Leak Waste Concept bench installation The waste tube connected to the leak pan outlet on each of the bottom instruments guides the solvent to a suitable waste container ...

Page 32: ...hyl formamide leads to corrosion of the leak sensor The material of the leak sensor PVDF polyvinylidene fluoride is incompatible with DMF Do not use DMF NOTE The leak sensor in the sampler is hidden under the ICC Column Heater or Column Shelf respectively NOTE The waste drainage must go straight into the waste containers The waste flow must not be restricted at bends or joints ...

Page 33: ...on 1 Leak and Waste Handling Waste Concept 1 Agilent recommends using the 6 L waste can with 1 Stay Safe cap GL45 with 4 ports 5043 1221 for optimal and safe waste disposal If you decide to use your own waste solution make sure that the tubes don t immerse in the liquid ...

Page 34: ...34 Agilent InfinityLab LC Series Vialsamplers User Manual 1 Introduction Leak and Waste Handling ...

Page 35: ...ance Specifications 41 Performance Specifications G7129A 41 Performance Specifications G7129B 43 Performance Specifications G7129C 45 Specifications of the Sample Cooler 47 Specifications of the Sample Thermostat 49 Specifications of the Integrated Column Compartment 51 This chapter provides information on environmental requirements physical and performance specifications ...

Page 36: ...an result if the devices are connected to a line voltage higher than specified Connect your instrument to the specified line voltage only WARNING Electrical shock hazard The module is partially energized when switched off as long as the power cord is plugged in The cover protects users from personal injuries for example electrical shock Do not open the cover Do not operate the instrument and disco...

Page 37: ... purposes can lead to personal injury or damage of electronic equipment Never use a power cord other than the one that Agilent shipped with this instrument Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC reg...

Page 38: ...sample thermostat and laboratory Keep open fire or sources of ignition away from the device Ensure a room size of 4 m3 1 m3 for every 8 g of R600a refrigerant inside of the sample thermostat Ensure adequate ventilation typical air exchange of 25 m3 h per m2 of laboratory floor area Keep all ventilation openings in the enclosure clear of obstructions Do not block the openings on the circumference o...

Page 39: ...mpler Condensation NOTE Agilent recommends that you install the HPLC instrument in the InfinityLab Flex Bench rack This option helps to save bench space as all modules can be placed into one single stack It also allows to easily relocate the instrument to another laboratory WARNING Heavy weight The module is heavy Carry the module at least with 2 people Avoid back strain or injury by following all...

Page 40: ...04 F without sample cooler or sample thermostat up to 55 C 131 F Ambient non operating temperature 40 70 C 40 158 F Humidity 95 r h at 40 C 104 F 1 1 If a sample cooler or thermostat is included the upper value for humidity can be reduced Please check your lab conditions to stay beyond dew point values for non condensing operation Non condensing Operating altitude Up to 3000 m 9842 ft Non operatin...

Page 41: ...tical Head Sample viscosity range 0 2 5 cp Sample capacity 132 x 2 mL vial two trays default 100 x 2 mL vial two classic trays optional 36 x 6 mL vials two trays optional Carry over 0 004 40 ppm with needle wash Injection cycle time 18 s for draw speed 200 µL min Ejection speed 200 µL min Injection volume 1 µL Minimum sample volume 1 µL from 5 µL sample in 100 µL microvial or 1 µL from 10 µL sampl...

Page 42: ...ror detection and display with Agilent Lab Advisor software Leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclabl...

Page 43: ...si Sample viscosity range 0 2 5 cp Sample capacity 132 x 2 mL vial two trays default 100 x 2 mL vial two classic trays optional 36 x 6 mL vials two trays optional Carry over 0 004 40 ppm with needle wash Injection cycle time 18 s for draw speed 200 µL min Ejection speed 200 µL min Injection volume 1 µL Minimum sample volume 1 µL from 5 µL sample in 100 µL microvial or 1 µL from 10 µL sample in 300...

Page 44: ...ab Advisor software Leak detection safe leak handling leak output signal for shutdown of pumping system and low voltages in major maintenance areas GLP features Early maintenance feedback EMF for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclable Table 3 Performance Specifications G71...

Page 45: ...d 200 µL min Injection volume 1 µL Minimum sample volume 1 µL from 5 µL sample in 100 µL microvial or 1 µL from 10 µL sample in 300 µL microvial Needle height offset has to be adapted to ensure that needle doesn t touch vial bottom Default needle height 0 equates to 2 mm above the vial bottom Instrument Control LC CE Drivers A 02 17 or above Instrument Control Framework ICF A 02 05 or above Instan...

Page 46: ...ations GLP features Early maintenance feedback EMF for continuous tracking of instrument usage with user settable limits and feedback messages Electronic records of maintenance and errors Housing All materials recyclable Table 4 Performance Specifications 1260 Infinity II Vialsampler G7129C Type Specification Comment ...

Page 47: ...bel Table 5 Physical Specification of the Sample Cooler Type Specification Comments Weight 6 kg Dimensions height width depth 205 mm x 340 mm x 370 mm Refrigerant gas HFC 134a 0 042 kg Ozone depletion potential ODP 0 Supply voltage 24 VDC nominal Current 10 A max Ambient operating temperature 4 40 C 39 2 104 F Ambient non operating temperature 40 70 C 20 158 F Humidity 95 r h at 40 C 104 F Non con...

Page 48: ...osed of in accordance with national and local regulations Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www agilent com for more info Table 6 Performance Specifications Agilent 1290 Sample Cooler Type Specifications Operating principle High performance low energy consumption micro compressor based cooler with ozone friendly HFC ...

Page 49: ...e manual Table 7 Physical Specifications of the Sample Thermostat Type Specification Comment Weight 6 kg Dimensions height x width x depth 205 mm x 340 mm x 370 mm Refrigerant gas R600a 0 030 kg Ozone depletion potential ODP 0 Global warming potential GWP 3 Supply voltage 24VDC nominal Current 10 A max Ambient operating temperature 4 40 C 39 2 104 F Ambient non operating temperature 40 70 C 20 158...

Page 50: ...lations Please contact your local Agilent Service Center in regard to safe environmental disposal of the appliance or check www agilent com for more info Table 8 Performance Specifications for the Sample Thermostat Type Specifications Operating principle High performance low energy consumption micro compressor based cooler with natural R600a coolant Butane 30 g user upgradable Temperature range fr...

Page 51: ...Supply Voltage 24 V DC Power consumption 110 W Ambient operating temperature 4 55 C 39 131 F Ambient non operating temperature 40 70 C 40 158 F Humidity 95 r h at 40 C 104 F 1 1 If a sample cooler or sample thermostat is included the upper value for humidity can be reduced Please check your lab conditions to stay beyond dew point values for non condensing operation Non condensing Operating altitud...

Page 52: ...mance Specifications Agilent InfinityLab LC Series Integrated Column Compartment G7130A Type Specification Comments Temperature range 5 C above ambient to 80 C Column capacity 2 columns up to 30 cm and 4 6 mm ID Temperature stability 0 10 C at sensor Temperature accuracy 0 8 K 0 5 K with calibration measured with water at ambient temperature 25 C set point at 40 C and a stable flow of 1 mL min at ...

Page 53: ... Install the Integrated Column Compartment 60 Install the Column Shelf 66 Install the External Tray 70 Install a Column ICC 72 Remove a Column ICC 76 Sample Trays 78 Choice of Vials and Caps 81 Transporting the Sampler with a Sample Cooler or Sample Thermostat Installed 84 Agilent Local Control Modules 86 This chapter provides information on how to use the module ...

Page 54: ...54 Agilent InfinityLab LC Series Vialsamplers User Manual 3 Using the Module Magnets Magnets 1 This stack exemplarily shows the magnets positions in the modules ...

Page 55: ...Agilent InfinityLab LC Series Vialsamplers User Manual 55 Using the Module 3 Turn on off Turn on off 1 2 Power switch On 3 Turn instrument On Off with the control software 4 Power switch Off ...

Page 56: ...56 Agilent InfinityLab LC Series Vialsamplers User Manual 3 Using the Module Turn on off 5 ...

Page 57: ...us indicators 1 Idle 2 Run mode 3 Not ready Waiting for a specific pre run condition to be reached or completed 4 Error mode interrupts the analysis and requires attention for example a leak or defective internal components 5 Resident mode blinking for example during update of main firmware 6 Bootloader mode fast blinking Try to re boot the module or try a cold start Then try a firmware update ...

Page 58: ...t operate the sampler without drawers installed NOTE Install all drawers for best cooling performance NOTE Do not mix standard and classic drawers Install classic drawer 1 50 to the left classic drawer 51 100 to the right side 1 Open the front door 2 Pull the drawer out 3 Lift the front of the drawer 4 Lift the drawer out ...

Page 59: ...gilent InfinityLab LC Series Vialsamplers User Manual 59 Using the Module 3 Exchange Drawers 5 Remove the drawer 6 Insert the back of the drawer 7 Align the drawer 8 Push in the drawer 9 Close the doors ...

Page 60: ...iption 1 G7130 60030 ICC Column Heater 3 µL volume OR 1 G7130 60060 ICC Column Heater 6 µL volume Preparations Switch off the pump Switch off the sampler Column is installed in the ICC Open the doors of the sampler WARNING Fire or explosion due to missing leak drainage Solvents may run into the module if Column Shelf or Integrated Column Compartment are not installed Install either Column Shelf or...

Page 61: ...lent InfinityLab LC Series Vialsamplers User Manual 61 Using the Module 3 Install the Integrated Column Compartment 1 Press the levers left and right 2 Pull out the column shelf The column shelf is removed ...

Page 62: ...3 Using the Module Install the Integrated Column Compartment 3 Push in the Integrated Column Compartment 4 Click in the levers The Integrated Column Compartment is installed 5 Push the latch of the Integrated Column Compartment to the left 6 Open the flap OLFN ...

Page 63: ...ampler to the Integrated Column Compartment 8 Connect the capillary to the heat exchanger 9 Tighten the capillary fitting to make the connection pressure tight NOTE Do not overtighten the fitting maximum torque 2 5 Nm NOTE The use of capillaries with SL or SX fittings are recommended for a better handling 10 Install the column holders to the heat exchanger PD 1P PD 1P ...

Page 64: ...rtight 12 Close the lever to make a pressure tight connection 13 Connect and install the fitting fingertight 14 Use a wrench to counter the column while tightening the capillary fitting 15 Position the column into the Column Holder Clip s 16 Route the capillary leading to the system between the latch and the Integrated Column Compartment NOTE Optionally route the capillary to the side ...

Page 65: ...finityLab LC Series Vialsamplers User Manual 65 Using the Module 3 Install the Integrated Column Compartment 17 Close the flap until the latch is locked 18 Close the doors 19 Turn on the module and the pump OLFN ...

Page 66: ...the pump Switch off the sampler Open the doors of the sampler Open the ICC WARNING Fire or explosion due to missing leak drainage Solvents may run into the module if Column Shelf or Integrated Column Compartment are not installed Install either Column Shelf or Integrated Column Compartment before starting the module 1 Remove the capillary connection from the integrated column compartment to the mo...

Page 67: ...Module 3 Install the Column Shelf 3 Unscrew the right fitting 4 Remove the right fitting 5 Open the lever of the InfinityLab Quick Connect Fitting 6 Unscrew and remove the InfinityLab Quick Connect Fitting 7 Column removed 8 Close the integrated column compartment OLFN ...

Page 68: ...nfinityLab LC Series Vialsamplers User Manual 3 Using the Module Install the Column Shelf 9 Press the levers left and right 10 Pull out the integrated column compartment The integrated column comparment is removed ...

Page 69: ...alsamplers User Manual 69 Using the Module 3 Install the Column Shelf 11 Push in the column shelf 12 Click in the column shelf to fix it properly The column shelf is installed 13 Close the doors 14 Turn on the module and the pump OLFN ...

Page 70: ...l Tray for 5 x 2 mL Vials G1313 27302 Disposal tube Preparations Turn the sampler off Open the doors Remove the samples NOTE Keep foam and plastic cover in a safe place For best temperature performance and if the external tray is not in use it is best to cover the opening for the external tray with the original parts 1 Lift the foam with a flathead screwdriver 2 Remove the foam ...

Page 71: ...ing the Module 3 Install the External Tray 3 Push out the plastic cover Vent for the external tray is open 4 Insert the external tray 5 Install the disposal tube 6 Close the door 7 Configure the External Tray in the online view of the Chromatographic Data System ...

Page 72: ... 16 inch Parts required p n Description Column up to 30 cm length 5067 5957 InfinityLab Quick Connect Assy ST 0 12 mm x 105 mm 5067 5966 InfinityLab Quick Turn fitting Preparations Switch off the pump Switch off the sampler Open the doors of the sampler NOTE A maximum of two columns can be installed 1 Push the latch of the Integrated Column Compartment to the left 2 Open the flap ...

Page 73: ...the Integrated Column Compartment 4 Connect the capillary to the heat exchanger 5 Tighten the capillary fitting to make the connection pressure tight NOTE Do not overtighten the fitting maximum torque 2 5 Nm NOTE The use of capillaries with SL or SX fittings are recommended for a better handling 6 Install the column holders to the heat exchanger PD 1P PD 1P ...

Page 74: ...lose the lever to make a pressure tight connection 9 Connect and install the fitting fingertight 10 Use a wrench to counter the column while tightening the capillary fitting 11 Position the column into the Column Holder Clip s 12 Route the capillary leading to the system between the latch and the Integrated Column Compartment NOTE Optionally route the capillary to the side ...

Page 75: ...Agilent InfinityLab LC Series Vialsamplers User Manual 75 Using the Module 3 Install a Column ICC 13 Close the flap until the latch is locked 14 Configure Columns for the ICC OLFN ...

Page 76: ... Open end wrench 1 4 5 16 inch Parts required p n Description Column up to 30 cm length 5067 5957 InfinityLab Quick Connect Assy ST 0 12 mm x 105 mm 5067 5966 InfinityLab Quick Turn fitting Preparations Switch off the pump Switch off the sampler Open the doors of the sampler 1 Push the latch 2 Open the flap 90 3 Remove the column 4 Unscrew the right fitting ...

Page 77: ...al 77 Using the Module 3 Remove a Column ICC 5 Remove the right fitting 6 Open the lever of the InfinityLab Quick Connect Fitting 7 Unscrew and remove the InfinityLab Quick Connect Fitting 8 Column removed 9 Close the flap until the latch is locked OLFN ...

Page 78: ...positions of the right hand drawer begin at position P2 A1 as follows Left hand Drawer for 66 x 2 mL Vials P1 A1 to P1 F11 Left hand Drawer for 18 x 6 mL Vials P1 A1 to P1 C6 Right hand Drawer for 66 x 2 mL Vials P2 A1 to P2 F11 Right hand Drawer for 18 x 6 mL Vials P2 A1 to P2 C6 Drawer for 50 x 2 mL Vials Classic Left Vial 1 50 Drawer for 50 x 2 mL Vials Classic Right Vial 51 100 External Tray 5...

Page 79: ...t into the backside of the hole position No 206 Figure 14 Numbering of drawer position left hand Drawer for 66 x 2 mL Vials Figure 15 Numbering of drawer position right hand Drawer for 66 x 2 mL Vials Figure 16 Numbering of drawer position left hand Drawer for 18 x 6 mL Vials Figure 17 Numbering of drawer position right hand Drawer for 18 x 6 mL Vials 3 3 3 3 3 3 3 3 ...

Page 80: ...finityLab LC Series Vialsamplers User Manual 3 Using the Module Sample Trays Figure 18 Numbering of drawer position Drawer for 50 x 2 mL Vials Classic Figure 19 Numbering of tray position External tray DVWH LVSRVDO ...

Page 81: ...th the autosampler Crimp Top Vials p n Description 5181 3375 Crimp Top Vial 2 mL clear glass 100 Pack 5183 4491 Crimp Top Vial 2 mL clear glass 1000 Pack 5182 0543 Crimp Top Vial 2 mL clear glass write on spot 100 Pack 5183 4492 Crimp Top Vial 2 mL clear glass write on spot 1000 Pack 5183 4494 Crimp Top Vial 2 mL clear glass write on spot 100 Pack silanized 5181 3376 Crimp Top Vial 2 mL amber glas...

Page 82: ...s write on spot 100 Pack 5183 4506 Snap Top Vial 2 mL amber glass write on spot 1000 Pack 5183 4509 Snap Top Vial 2 mL amber glass write on spot 100 Pack silanized p n Description 5182 0714 Screw Cap Vials 2 mL clear glass 100 Pack 5183 2067 Screw Top Vial 2 mL clear glass 1000 Pack 5183 2070 Screw Top Vial 2 mL clear glass 100 Pack silanized 5182 0715 Screw Top Vial 2 mL clear glass write on spot...

Page 83: ...ar polypropylene septum clear PTFE red rubber 100 Pack 5182 3458 Snap Cap blue polypropylene septum clear PTFE red rubber 100 Pack 5182 3457 Snap Cap green polypropylene septum clear PTFE red rubber 100 Pack 5182 3459 Snap Cap red polypropylene septum clear PTFE red rubber 100 Pack p n Description 5182 0717 Screw Cap blue polypropylene septum clear PTFE red rubber 100 Pack 5182 0718 Screw Cap gree...

Page 84: ... the module Otherwise no special precautions are needed for the modules WARNING Heavy weight The module is heavy Carry the module at least with 2 people Avoid back strain or injury by following all precautions for lifting heavy objects Ensure that the load is as close to your body as possible Ensure that you can cope with the weight of your load WARNING Flammable refrigerant Formation of flammable...

Page 85: ... all vials from the drawers The condensed water inside of the sample cooler or sample thermostat is removed NOTE Transporting the sampler with a sample cooler or sample thermostat installed is only allowed for short distances For longer distances you must separate the units and send them independently CAUTION Mechanical damage of the module If the transport assembly is not parked and not protected...

Page 86: ...toring of Agilent Infinity II Prime LC Modules Excellent usability and ease of use through a user interface specifically tailored for mobile devices simple intuitive touch enabled and visual controllable High flexibility through a modern Bring your own device approach Connection between LC module and mobile device either wireless via WLAN or wired over USB cable with full package Convenient ergono...

Page 87: ...00 Series 1200 Series SL and 1100 Series Excellent readability and usability by large colored display with background light high resolution and contrast Convenient ergonomic operation either handheld or at the stack with newly developed secure attachment Handheld or attached to a module in a stack to facilitate operator preferences The 1200 Infinity Series Instant Pilot provides Easy automation re...

Page 88: ...88 Agilent InfinityLab LC Series Vialsamplers User Manual 3 Using the Module Agilent Local Control Modules ...

Page 89: ...aged Packaging 90 Install the Sample Cooler or Thermostat 91 Using the Sample Cooler or Thermostat in an Infinity II Sampler 97 Control Interface 98 Control 99 Temperature Mode 100 Online Signal Monitor 101 Operation Information 102 Important Information 103 This chapter describes the installation of the optional sample cooler or thermostat ...

Page 90: ...ediately Inform your service representative that the instrument may have been damaged during shipment CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage An Agilent service representa...

Page 91: ...ck WARNING Flammable refrigerant Formation of flammable gas air mixtures inside the sample thermostat and laboratory Keep open fire or sources of ignition away from the device Ensure a room size of 4 m3 1 m3 for every 8 g of R600a refrigerant inside of the sample thermostat Ensure adequate ventilation typical air exchange of 25 m3 h per m2 of laboratory floor area Keep all ventilation openings in ...

Page 92: ...no serviceable parts inside NOTE If the sample cooler or thermostat is disconnected from the power supply you should wait for at least five minutes before replugging and switching on the compressor again NOTE Even under average humidity conditions a significant amount of condensed water gathers every day A suitable container must be provided and emptied regularly in order to avoid overflow NOTE Fo...

Page 93: ...r or Thermostat 4 Install the Sample Cooler or Thermostat 1 Ensure that the power switch on the front of the module is OFF switch stands out 2 Ensure that the power cable is removed from the instrument 3 Open the 4 screws on the rear of the module 4 Remove the sample cooler thermostat mainframe cover ...

Page 94: ... Do not use the sample cooler or thermostat if it is not operating correctly or has been damaged Disconnect it from the power supply and call your local service center Remove the power cable from the module before opening the cover Do not connect the power cable to the module while the covers are removed If the sample cooler or thermostat is disconnected from the power supply you should wait for a...

Page 95: ...stat CAUTION Damage to the cables Do not bend or pinch the cables Fit in the sample cooler or thermostat perfectly 7 Slide in the whole unit 8 Tighten the four screws which hold the sample cooler or thermostat unit in place 9 Use a bubble level to check the leveling of the sampler NOTE The module should be operated in a proper horizontal position 10 PP ...

Page 96: ...12 NOTE Check leak waste handling for further info CAUTION Damage to the sample cooler or thermostat Wait at least 30 min before switching on the module This allows the refrigerant and system lubrication to reach equilibrium 13 Connect the power cable to the power connector at the rear of the module 14 Configure the Sample Cooler or Thermostat in the CDS PP PP ...

Page 97: ...er or sample thermostat in the multisampler The principle is the same for other sampler types After successfully loading the Agilent CDS the module should appear as an active item in the graphical user interface GUI Within the autosampler GUI there are active areas If the mouse cursor is moved across the icons the cursor will change see the following figure 1 ALS turn on and off 2 ALS configuratio...

Page 98: ...sor over the syringe The information provides ALS related information like Part number ALS setup and other details Control Interface A right click into the active area will open a menu to show the Control Interface NOTE Depending on the autosampler temperature set point and the environment the autosampler temperature can deviate by up to 3 degrees If the system is not in the range of 2 C you will ...

Page 99: ... the Control dialog box is available when you have a sample cooler or sample thermostat installed It allows you to switch the cooler or thermostat on and off manually Select On to switch on the cooler thermostat Specify the required temperature in the adjacent field Note that the specified temperature must be at least 5 C below ambient for proper temperature control Select Off to switch off the co...

Page 100: ... one in the current method The sample temperature can not be directly controlled only the temperature of the air stream from the thermostat If the air stream has the correct temperature during method execution it may have been stored at higher temperatures with previous methods As a result we are now introducing two modes which can be configured in the dashboard Constant temperature mode this mode...

Page 101: ... Sample Cooler or Thermostat in an Infinity II Sampler Online Signal Monitor The online signal monitor for the chiller is also available and allows you to monitor the WPS1A temperature signals The Online Plot Window should look like this If the change button is pressed you can select the Chiller signal WPS1A in the online plot ...

Page 102: ...drawers If the doors are opened frequently warm and or humid air enters the workspace of the autosampler Try to limit opening the door drawers in extremely hot or humid weather Temperature rise Due to the warm air temperatures generated during heating from a cold to a warm chiller set point it is possible to see moisture forming on the shelves and the vials This moisture is caused by the warm air ...

Page 103: ... necessary to avoid icing inside the chiller If you turn the sample cooler or sample thermostat off a Remove all sample containers or vials from the autosampler b Let the autosampler temperature stabilize to ambient temperature open the door far enough for air to get in c Clean the drawers of the sample hotel or the cold reservoir underneath the drawers in the sampler wipe them down and dry them w...

Page 104: ...104 Agilent InfinityLab LC Series Vialsamplers User Manual 4 Installation of the Optional Sample Cooler or Thermostat Using the Sample Cooler or Thermostat in an Infinity II Sampler ...

Page 105: ...ing the Module 107 Solvent Information 108 Capillary Color Coding Guide 114 Installing Capillaries 115 Flow Connections to the Vialsampler 117 Setting up the Vialsampler 119 Control Settings 122 Method Parameter Settings 124 Injector Programm 128 Module Configuration View 129 This chapter explains the operational parameters of the module ...

Page 106: ...ature in the sample compartment at least 25 K below the boiling point of the solvent used Do not operate the instrument in an explosive atmosphere Do not use solvents of ignition Class IIC according IEC 60079 20 1 for example carbon disulfide Reduce the volume of substances to the minimum required for the analysis Never exceed the maximum permissible volume of solvents 8 L in the solvent cabinet D...

Page 107: ...olvents see Solvent Information on page 108 Priming and Purging the System When the solvents have been exchanged or the system has been turned off for a certain time for example overnight oxygen will re diffuse into the solvent channel Therefore priming and purging of the system is required before starting an application Table 11 Choice of Priming Solvents for Different Purposes Activity Solvent C...

Page 108: ...materials like flow cells valve materials etc and recommendations in subsequent sections Recommended Wash Solvents water ethanol methanol water acid especially for basic compounds water base especially for acidic compounds water acetonitrile Material Information Materials in the flow path are carefully selected based on Agilent s experiences in developing highest quality instruments for HPLC analy...

Page 109: ...al compatibility of materials PEEK PEEK Polyether Ether Ketones combines excellent properties regarding biocompatibility chemical resistance mechanical and thermal stability PEEK is therefore the material of choice for UHPLC and biochemical instrumentation It is stable in the specified pH range for the Bio inert LC system pH 1 13 see bio inert module manuals for details and inert to many common so...

Page 110: ...lvents used in chromatographic systems like methanol acetonitrile and isopropanol It has limited stability with aliphatic aromatic and halogenated hydrocarbons THF phenol and derivatives concentrated acids and bases For normal phase applications the maximum pressure should be limited to 200 bar Tantalum Ta Tantalum is inert to most common HPLC solvents and almost all acids except fluoric acid and ...

Page 111: ... is highly resistant to oxidizing acids for example nitric perchloric and hypochlorous acid over a wide range of concentrations and temperatures This is due to a thin oxide layer on the surface which is stabilized by oxidizing compounds Non oxidizing acids for example hydrochloric sulfuric and phosphoric acid can cause slight corrosion which increases with acid concentration and temperature For ex...

Page 112: ...m Platinum Iridium is inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications Fluorinated polymers PTFE PFA FEP FFKM PVDF Fluorinated polymers like PTFE polytetrafluorethylene PFA perfluoroalkoxy and FEP fluorinated ethylene propylene are inert to almost all common acids bases and solvents FFKM is perfluorinated rubber which is also resis...

Page 113: ... Preparing the Module 5 Solvent Information Sapphire Ruby and Al2O3 based ceramics Sapphire ruby and ceramics based on aluminum oxide Al2O3 are inert to almost all common acids bases and solvents There are no documented incompatibilities for HPLC applications ...

Page 114: ...114 Agilent InfinityLab LC Series Vialsamplers User Manual 5 Preparing the Module Capillary Color Coding Guide Capillary Color Coding Guide Figure 20 Syntax for capillary description ...

Page 115: ...es mentioned above are examples only Parts required p n Description 5067 4650 Capillary ST 0 12 mm x 150 mm SL SX 5067 4651 Capillary ST 0 12 mm x 280 mm SL SX 5067 4720 Capillary ST 0 17 mm x 150 mm SL SX 5067 4722 Capillary ST 0 17 mm x 280 mm SL SX 5065 4454 Fitting screw long 10 pk Quantity depends on configuration of the module number of connections to the multisampler 1 Select a nut that is ...

Page 116: ...he fitting which forces the ferrule to seat onto the tubing or capillary NOTE Don t overtighten Overtightening will shorten the lifetime of the fitting 5 Loosen the nut and verify that the ferrule is correctly positioned on the tubing or capillary NOTE The first time that the swagelock fitting is used on a column or an injection valve the position of the ferrule is permanently set If changing from...

Page 117: ...ner 5 Prime the wash tubing 6 Install the capillary from the pump outlet into the port 1 of the injection valve Preparations Module is installed in the system Use an appropriate solvent based on the sample and mobile phase chemistries The composition of the wash solvent should be the most solubilizing compatible solvent your strongest diluent Selecting the wash solvent is part of the method develo...

Page 118: ...paring the Module Flow Connections to the Vialsampler 7 Install the capillary from port 6 of the injection valve to the ICC or MCT The correct plumbing is shown in the figure below and is attached to the module 8 Prime and purge the complete sampler DVWH ROXPQ 3XPS ...

Page 119: ...ing on the controller e g Agilent Local Controller OpenLab EZChrom Masshunter the screens look different Figure 21 ChemStation Method and Run Control After successful load of the OpenLab ChemStation you should see the module as an active item in the graphical user interface GUI NOTE This section describes the Vialsampler settings only For information on the software or other modules refer to the c...

Page 120: ...a will open a menu with the option to adjust the following parameters Show the Control User Interface special module settings See Control Settings on page 122 Show the Method User interface same as via menu Instrument Set up Instrument Method Setup G7129B See Method Parameter Settings on page 124 Injector Program When you activate a pretreatment injector program it replaces the standard injection ...

Page 121: ...un Ready Error state and Not Ready text or Error text Offline gray Ok No Maintenance required green EMF warning Maintenance might be required yellow EMF warning Maintenance required red Table 12 The Vialsampler User Interface NOTE For additional help and support highlight the desired area and press the F1 key A help screen will open with additional information and documentation about the topic NOT...

Page 122: ...C Series Vialsamplers User Manual 5 Preparing the Module Setting up the Vialsampler Control Settings The control settings are available via right click on the active area of the grafical user interface see Table 12 on page 120 ...

Page 123: ... temperature control Select Off to switch off the cooler thermostat Automatic Turn On You can set a date and time at which the cooler thermostat switches on automatically Pump connected to Sampler Use this section to specify the pump that is used with the Vialsampler If more than one pump is configured display the drop down list and select the appropriate pump from the list Enable Analysis This se...

Page 124: ...amplers User Manual 5 Preparing the Module Setting up the Vialsampler Method Parameter Settings The method parameter settings are available via right click on the active area of the grafical user interface Figure 23 Method parameter settings ...

Page 125: ...is enabled only if you have selected Flush Port Specify a flush time in seconds between 1 and 100 in steps of 1 Location enabled only if you have selected a Wash Vial Allows you to specify a wash location in the format Vial X where X is the vial number Specify well locations in the format P Plate Row Character Column Number for example P1 A1 The values of Row Character and Column Number depend on ...

Page 126: ...d for an injection cycle For viscous samples in combination with multidraw use a slow Eject Speed Wait Time After Draw ensures that the temporary vacuum which originates from the drawing of liquid from the sample vial dissipates The needle stays first on the seat for the specified time then after drawing sample from the vial remains there for the specified time Needle Height Offset a vertical offs...

Page 127: ... to obtain the desired degree of flushing to prevent sample carry over Overlapped Injection Mode provides faster throughput of samples by allowing the preparation of the next injection while the current injection is being completed The following options can be selected from the drop down list Off to switch off overlapped injection Select When sample is Flushed Out to specify that the sample can be...

Page 128: ...jector program comprises a series of numbered lines each specifying an operation that the autosampler carries out sequentially When you activate a pretreatment injector program it replaces the standard injection cycle The following functions are available Draw Eject Mix Inject Move Wait Valve Needle Wash Remote Wait For Repeat End Repeat Comment ...

Page 129: ...ngs are available via menu Instrument Show ConfigUI Figure 24 Configuration view NOTE For additional help and support Highlight the desired area and press the F1 key A help screen will open with additional information and documentation about the topic NOTE The vialsampler configuration is done in the module dashboard context menu not in the instrument configuration ...

Page 130: ...130 Agilent InfinityLab LC Series Vialsamplers User Manual 5 Preparing the Module Setting up the Vialsampler ...

Page 131: ...ash Wash Port 132 Using an Injector Program 134 General Recommendation to Lowest Carry Over 135 Fast Injection Cycle and Low Delay Volume 136 Overlapped Injection Mode 136 General Recommendations for Fast Injection Cycle Times 137 Precise Injection Volume 138 Draw and Eject Speed 138 Choice of Rotor Seal 140 This chapter provides information on how to optimize the module ...

Page 132: ...oncentration carry over may become noticeable Using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Using the Automated Needle Wash Wash Port The automated needle wash can be programmed either as method parameter enable needle wash or the needle wash can be included into the injector program When the automated needle wash is used ...

Page 133: ... use an injector program and an additional wash vial with an appropriate and stronger solvent for cleaning The wash vial should contain solvent in which the sample components are soluble and the vial should not be capped If the wash vial is capped small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Injector Program with Needle Wash The ...

Page 134: ...e to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are used Example Outside wash of needle in vial p2 A1 before injection Injector program Draw x x y μl from sample WASH vial location p2 A1 Inject Wait equilibration time see text above Valve bypass Wait 0 2 min Valve mainpass main...

Page 135: ...g procedure can be used to clean the needle seat In Lab Advisor go to Maintenance Change Needle this will set the needle into home position Remove the safety cover of the sampler Be carefully not to risk an injury by the uncovered needle Pipette an appropriate solvent on to the needle seat The solvent should be able to dissolve the contamination If the contamination is unknown use 2 or 3 solvents ...

Page 136: ... cycle starts but waits with switching to mainpass main path until the actual run is finished You gain the sample preparation time when using this process Switching the valve into the bypass position reduces the system delay volume the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow ...

Page 137: ...Add at last run a blank if overlapped injection is used To reduce the injection time the detector balance has to be set to OFF NOTE For the last injection of the sequence with overlapped injections it has to be considered that for this run the injection valve is not switched as for the previous runs and consequently the injector delay volume is not bypassed This means the retention times are prolo...

Page 138: ...ection volumes 2 μL If the pump has no degasser it is recommended to use an Agilent 1260 Infinity degasser Also using the automated needle wash see Optimization for Lowest Carry over on page 132 between injections reduces carry over to a minimum further improving the injection volume precision Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may hav...

Page 139: ...lue speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the piston returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program When using viscous sam...

Page 140: ...le for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime PEEK Seal for 1260 injection valve only The 1260 injection valve has a sealing material made of PEEK This material has high chemical resistance and versatility It is suitable for application using mobile phases within a ...

Page 141: ...l or Piston 147 Park Arm 148 Change Gripper 150 Automatic Referencing 151 Vialsampler Gripper Verification Test 152 Step Commands 153 Tests 155 ICC Heater Test 155 Sample Cooler Function Test 155 Troubleshooting 156 Troubleshooting Guide for the Sample Transport Assembly 158 Intermittent lock ups with or without vial in the gripper fingers 159 Poor alignment 160 Agilent Lab Advisor Software 161 Ov...

Page 142: ... failure the module generates an error message in the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided see chapter Error Information Maintenance Functions The maintenance functions position the needle arm gripper assembly and metering device for easy access when doing maintena...

Page 143: ... Module s Indicators and Test Functions Step Commands The step functions provide the possibility to execute each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see Step Commands on page 153 ...

Page 144: ...e positions can be selected from the Service Diagnostic Maintenance Positions menu in the Diagnosis display User Interface The functions for the control software are Change Needle Sample Loop or Needle Seat The needle moves slowly down and positions the needle arm for easy access to the needle the sample loop or the needle seat Change Metering Seal or Piston relieves the tension on the metering sp...

Page 145: ...e arm moves in the desired position and guide the user to the next procedure step Move Down The needle arm moves slowly down Move Up The needle arm slowly moves up Back Completes the needle needle seat procedure WARNING For the needle exchange procedure the needle arm moves slowly down while the safety cover is removed Risk of personal injury due to moving needle Keep fingers off the moving needle...

Page 146: ...needle seat assembly After the exchanging of the needle seat reinstall the new needle assembly See Exchange the Needle Assembly on page 192 and Exchange the Needle Seat Assembly on page 197 5 Next positions the needle to the top level Flap the safety wash port back in its origin position 6 Reinstall the safety cover and complete the procedure 7 Select Next to move the needle into the needle seat 8...

Page 147: ... the control software are Start Draws the piston away from the home position relieving the tension on the spring Next Repositions the piston at the home position Back Completes the procedure Using the Change Seal Function 1 Select Start to move the piston to the maintenance position 2 Exchange the metering seal see Exchange the Metering Seal and Piston on page 212 3 Select Next to move the piston ...

Page 148: ...erface In the control software the Park Arm command is part of the ALS positions that can be selected from the special commands menu in the Instrument Control display The commands for the control software are Park Arm moves the gripper arm to the park position Home moves the gripper arm out of the park position to the home position ...

Page 149: ...reparation 1 Select Park Arm 2 When the arm is in the park position install Transport Protection G7129 40050 Then the autosampler is ready for shipment and can be switched OFF When Before transporting or shipping the module CAUTION Unsecured Transportation of the module Unsecured transportation of the module may result in mechanical damage to the gripper and transport slider Always secure the arm ...

Page 150: ... transport assembly and gripper arm to the position required to change the gripper arm Next Repositions the transport assembly and gripper arm to the home position Back Completes the procedure Using the Change Gripper Function 1 Select Start to move the gripper arm to the maintenance position 2 Exchange the gripper arm see Exchange the Gripper Arm on page 216 3 Select Next to move the gripper arm ...

Page 151: ...nts Because the tray is a rectangle a four point alignment is sufficient to correct all other vial positions within the tray On completion of the procedure the corrected gripper positions are stored in the instrument firmware Start Moves the transport assembly and gripper arm to the position required to install the calibration tools Next Mount the calibration fixture Position P1 C6 and P2 F6 Next ...

Page 152: ...alsampler Gripper Verification Test Gripper Verification Description The verification procedure uses several vial positions as reference points to verify the gripper alignment is correct If verification indicates one or more positions are out of alignment the Automatic Referencing procedure should be done Allows you to select a vial position You can select from positions 1 11 39 56 66 101 111 139 ...

Page 153: ...ep Action Comments Valve Bypass Switches injection valve to the bypass position Piston Home Moves the piston to the home position Needle Up Lifts the needle arm to the upper position Command also switches the valve to bypass if it is not already in that position Vial to Seat Moves the selected vial to the seat position Command also lifts the needle to the upper position Needle into Sample Lowers t...

Page 154: ...not already in that position Vial to Tray Returns the selected vial to the tray position Command also lifts the needle to the upper position Needle into Seat Lowers the needle arm into the seat Command also returns the vial to the tray position Valve Mainpass Switches the injection valve to the mainpass main path position Reset Resets the injector Table 15 Injector step commands Step Action Commen...

Page 155: ... C but below 50 C the current oven temperature is used as the start temperature If the current oven temperature is above 50 C an error message is displayed The oven must then be allowed to cool to below 50 C before the test can be run Sample Cooler Function Test The Sample Cooler Function Test can be used as a simple verification that the sample cooler is functioning After the test has been starte...

Page 156: ...ective injection valve Piston Home Defective or dirty sensor on the hydraulic unit flex board Defective metering drive motor Needle Up Needle already in the upper position Defective or dirty sensor on the needle station flex board Sticking needle arm assembly Defective needle drive motor Vial to Seat No vial in selected position Vial already in seat position Defective transport assembly motors Sti...

Page 157: ...le station flex board Sticking needle arm assembly Defective needle drive motor Mainpass main path Valve already in mainpass main path Valve not connected Defective injection valve Needle Up Mainpass main path Blockage in the sample loop or needle no solvent flow Needle already in the upper position Defective or dirty sensor on the needle station flex board Sticking needle arm assembly Defective n...

Page 158: ...r without vial in the gripper fingers with error messages Many times the sampler is being used very heavily motor overtemp 0 or 1 or 2 or 3 movement failed 0 or 1 or 2 or 3 missing vial 2 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial with error messages motor overtemp 0 or 2 movement failed 0 or 2 3 Poor alignment seen during vial p...

Page 159: ... see Exchange the Gripper Arm on page 216 4 Start automatic referencing in Lab Advisor For this procedure it is mandatory to use the calibration tool On completion of the procedure the corrected values are stored in the instrument firmware 5 Exchange Transport assembly Sample Transport Assembly G7129 60600 6 Exchange the Autosampler Main Board Autosampler Specific Board G7129 65800 or the fusion b...

Page 160: ...G1313 60010 see Exchange the Gripper Arm on page 216 3 Exchange Transport assembly Sample Transport Assembly G7129 60600 4 Exchange the Autosampler Main Board Autosampler Specific Board G7129 65800 or the fusion board Fusion Board G4200 65880 5 For all three steps step 2 3 and 4 you have to do an automatic referencing again to store the correct values in the instrument firmware again WARNING Perso...

Page 161: ...ry Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument The Lab Advisor Advanced features can be unlocked by purchasing a license key and include real time monitoring of instrument actuals all various instrument signals and state machines In addition all diagnostic test results calibration results and acquired signal data can be uploaded to a shared network folder The Review C...

Page 162: ...162 Agilent InfinityLab LC Series Vialsamplers User Manual 7 Troubleshooting and Diagnostics Agilent Lab Advisor Software ...

Page 163: ...n 169 Leak Sensor Short 169 Compensation Sensor Open 170 Compensation Sensor Short 170 Fan Failed 171 Sampler Error Messages 172 Defective Gripper Assembly 172 Valve Failed 172 Needle Movement Failed 173 Missing Vial 173 Initialization Failed 174 Metering Home Failed 175 Motor Temperature 176 Initialization with Vial 177 Vial in Gripper 177 Missing Wash Vial 178 Invalid Vial Position 178 Valve swi...

Page 164: ...arget temperatur 181 Cooler Overpressure Failure 181 Cooler Condensate Error 182 Integrated Column Compartment ICC Heater Error Messages 183 ICC Temp Wait Timeout Error 183 ICC Heater Overcurrent Failure 183 ICC Heater Temp Sensor Failure 184 ICC Heater Over Temp Failure 184 This chapter describes the meaning of error messages and provides information on probable causes and suggested actions how t...

Page 165: ...a notification all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detector the lamp will stay on in order to avoid equilibration time Depending on the error type the next run can only be started if the error has been resolved for example liquid from a leak has been d...

Page 166: ...t ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required Probable cause Suggested actions 1 Leak detected in another module with a CAN connection to the system Fix the leak in the external instrument before restarting the modul...

Page 167: ...em configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable cause Suggested actions 1 Not ready condition in one of the instruments connected to the remote line Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the rem...

Page 168: ...s changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board Probable cause Suggested actions 1 Loose fittings Ensure all fittings are tight 2 Broken capillary Exchange defective capillaries 3 Leaking rotor seal or needle seat Exchange the rotor seal or seat capillary 4 Defective metering seal Exchange the metering seal Make sure the leak sensor is thoro...

Page 169: ... defined limits If the current increases above the upper limit the error message is generated Probable cause Suggested actions 1 Leak sensor not connected to the Power Switch board Please contact your Agilent service representative 2 Defective leak sensor Please contact your Agilent service representative 3 Leak sensor incorrectly routed being pinched by a metal component Please contact your Agile...

Page 170: ...ower switch board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the power switch board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable cause Suggested a...

Page 171: ...he error message is generated This limit is given by 2 revolutions second for longer than 5 seconds Depending on the module assemblies e g the lamp in the detector are turned off to assure that the module does not overheat inside Probable cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service represent...

Page 172: ...switch does not close the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 High friction in the transport assembly Please contact your Agilent service representative 3 Defective motor assembly Please contact your Agilent service representative 4 Defective sample transport assembly flex board Please contact...

Page 173: ...or encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable cause Suggested actions 1 Defective or dirty position sensor Please contact your Agilent service representative 2 Defective motor Please contact y...

Page 174: ... and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 Defective needle station board Please contact your Agilent service representative 3 Defective transport assembly flex board Please cont...

Page 175: ...to the home position or if the sensor fails to recognize the piston position the error message is generated Probable cause Suggested actions 1 Blockage in the flow path Change needle or needle seat 2 Defective sensor Please contact your Agilent service representative 3 Broken piston Exchange the metering piston and seal 4 Defective metering drive motor Please contact your Agilent service represent...

Page 176: ...current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable cause Suggested actions 1 Mechanical obstruction Ensure unobstructed movement of the transport assembly 2 ...

Page 177: ... Vial in Gripper Error ID 34308 The gripper arm attempted to move with a vial still in the gripper During specific stages of the sampling sequence no vial should be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is generated...

Page 178: ...assembly flex board are used to automatically check which sample trays are installed coding on tray If the vial position does not exist in the current sample tray configuration the error message is generated Probable cause Suggested actions 1 No wash vial in the position defined in the method Install the wash vial in the correct position or edit the method accordingly Probable cause Suggested acti...

Page 179: ...f the piston fails to move to the home position or if the metering device is not recognized by the instrument Probable cause Suggested actions 1 Defective rotor seal stator head Exchange the rotor seal stator head 2 Defective injection valve Please contact your Agilent service representative Probable cause Suggested actions 1 Metering device is not recognized Configure the metering device in LabAd...

Page 180: ...rd seems to be defective Probable cause Suggested actions 1 Blown fuses defective cable Check the power cable from the ALS to the sample cooler Please contact your Agilent service representative 2 Voltage issue in the sample cooler Please contact your Agilent service representative Probable cause Suggested actions 1 Blown fuses defective cable Check the power cable from the ALS to the sample coole...

Page 181: ...mple cooler pressure sensor has detected an overpressure Probable cause Suggested actions 1 Condensate inside of the chiller Check if the drainage is ok 2 Defective cable Check the signal cable from the ALS to the sample cooler 3 Defective temperature sensor Replace the sample cooler Probable cause Suggested actions 1 Overpressure in the chiller Switch off the instrument and wait for 15 min Restar...

Page 182: ...de of the leak pane Probable cause Suggested actions 1 Blocked drainage Switch off the instrument and remove the condensate tubings Place a beaker underneath the waste outlet Check the waste container There should be no kinked and sealed tubing 2 Defective condensate sensor Switch the sample cooler off on and check if after 8 min the error shows up again 3 Defective leak sensor Replace the sample ...

Page 183: ...time limit ICC Heater Overcurrent Failure Error ID 30821 The Integrated Column Compartment ICC Heater has detected an overcurrent in the heater circuits For safety reason heater over current protection became active Probable cause Suggested actions 1 Defective cable or connector Please contact your Agilent service representative 2 Defective ICC heater Replace the ICC heater Probable cause Suggeste...

Page 184: ...nt ICC Heater Over Temp Failure Error ID 30824 The Integrated Column Compartment ICC Heater has detected a temperature reading above 100 C This exceeds the limit of the heating element Probable cause Suggested actions 1 Defective cable or connector Please contact your Agilent service representative 2 Defective ICC heater sensor Replace the ICC heater Probable cause Suggested actions 1 Defective ca...

Page 185: ... Remove and Install Doors 191 Exchange the Needle Assembly 192 Exchange the Needle Seat Assembly 197 Exchange the Sample Loop Assembly 202 Exchange the Rotor Seal 206 Exchange the Metering Seal and Piston 212 Exchange the Gripper Arm 216 Replace the Peristaltic Pump Cartridge 219 Exchange the Wash Port Assembly 222 Replace the Module Firmware 228 This chapter describes the maintenance and the repa...

Page 186: ...ction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack Figure 25 Overview of the Vialsampler 6WDWXV LQGLFDWRU 1HHGOH DVVHPEO UDZHU ROXPQ VKHOI HDN GUDLQ 3RZHU VZLWFK NOTE There are no serviceable parts inside Do not open the module ...

Page 187: ...ent product user guides or use of the products in violation of applicable laws rules or regulations Use your Agilent products only in the manner described in the Agilent product user guides WARNING Electrical shock Repair work at the module can lead to personal injuries e g shock hazard when the cover is opened Do not remove the cover of the module Only certified persons are authorized to carry ou...

Page 188: ...w good laboratory practice The volume of substances should be reduced to the minimum required for the analysis Do not operate the instrument in an explosive atmosphere CAUTION Safety standards for external equipment If you connect external equipment to the instrument make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of extern...

Page 189: ... service representatives on a contractual basis For information regarding the maintenance inspection contract contact your Agilent representative The following pages describe the maintenance simple repairs of the module that can be carried out without opening the main cover Table 17 Overview of maintenance Procedure Typical interval minimum Notes Change needle needle seat 30000 needle into seat mo...

Page 190: ...le case clean use a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the module Do not use an excessively damp cloth during cleaning Drain all solvent lines before opening any connections in the flow path ...

Page 191: ...quired Description Flat screwdriver Parts required p n Description G7129 68702 Door Assy Preparations Finish any pending acquisition job and return any plate on the workspace back to the hotel 1 Press the release buttons and pull the front door out 2 For the Installation of the front door insert the hinges into their guides and move the door in until the release buttons click into their final posi...

Page 192: ...ubings from the solvent bottles If available close the shutoff valves WARNING Toxic flammable and hazardous solvents samples and reagents The handling of solvents samples and reagents can hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing as described in the material handling and...

Page 193: ...tem screen Tools Maintenance Positions Change Needle Loop click Start and wait until the needle assembly is in maintenance position 2 Open the front door 3 Unscrew the safety cover screw WARNING Sharp needle Uncovered needles may cause injuries Do not touch the tip of the needle 4 Remove the safety cover WARNING Sharp needle Uncovered needles may cause injuries Do not change the needle seat at thi...

Page 194: ...own position The needle will move slowly downwards and will stop approximately 2 mm above the seat 7 Disconnect the sample loop from the needle 8 Loosen the fixing screw 9 Lift out the needle 10 Insert the needle 1 Align the needle in the seat then tighten the screw firmly 2 11 Align the needle in the seat needle may be bent slightly manually if not aligned properly ...

Page 195: ... to the needle NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and breaking the sample loop 13 In Lab Advisor use Next to lift the needle slowly into the up position 14 Turn or flap the wash port to the right 15 Install the safety cover 1 and fix the screw 2 ...

Page 196: ...C Series Vialsamplers User Manual 9 Maintenance and Repair Exchange the Needle Assembly 16 Select Next to move the needle into the needle seat 17 Select Back to finish the procedure 18 Close the doors 19 Perform a pressure test ...

Page 197: ...ssembly 0 17 mm Vespel 600 bar OR 1 G7129 87112 Needle Seat Assembly 0 12 mm Vespel 600 bar Preparations Finish any pending acquisition job and in order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator Do not open the safety cove...

Page 198: ...ystem screen Tools Maintenance Positions Change Needle Loop click Start and wait until the needle assembly is in maintenance position 2 Open the front door 3 Unscrew the safety cover screw WARNING Sharp needle Uncovered needles may cause injuries Do not touch the tip of the needle 4 Remove the safety cover WARNING Sharp needle Uncovered needles may cause injuries Do not change the needle seat at t...

Page 199: ...ve the needle in the down position The needle will move slowly downwards and will stop approximately 2 mm above the seat 7 Disconnect the sample loop from the needle 8 Loosen the fixing screw 9 Lift out the needle 10 Disconnect the seat capillary fitting from the injection valve port 5 11 Use a small flat head screwdriver to ease out the needle seat ...

Page 200: ...mly into position 13 Connect the seat capillary fitting to port 5 of the injection valve 14 Insert the needle 1 Align the needle in the seat then tighten the screw firmly 2 15 Reconnect the sample loop to the needle NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and breaking the sample loop ...

Page 201: ...sembly 16 In Lab Advisor use Next to lift the needle slowly into the up position 17 Turn or flap the wash port to the right 18 Install the safety cover 1 and fix the screw 2 19 Select Next to move the needle into the needle seat 20 Select Back to finish the procedure 21 Close the doors 22 Perform a pressure test ...

Page 202: ... Loop 20 µL Preparations Finish any pending acquisition job and in order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator Do not open the safety cover of the needle station during normal operation Wear safety goggles and safety g...

Page 203: ...the Agilent Lab Advisor software select Service Diagnostics in the system screen Maintenance Positions Change loop click Start and wait until the needle assembly is in maintenance position 2 Open the front door 3 Unscrew the safety cover screw WARNING Sharp needle Uncovered needles may cause injuries Do not touch the tip of the needle 4 Remove the safety cover 5 Check if the needle is in maintenan...

Page 204: ...new sample loop NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and breaking the sample loop 8 After installing the new sample loop check the needle tip position by using the Needle Control function in Lab Advisor 9 Click on Next in the LabAdvisor Needle lift up again 10 Turn or flap the wash port to the right 11 Install the safety cover 1 and fix t...

Page 205: ...samplers User Manual 205 Maintenance and Repair 9 Exchange the Sample Loop Assembly 12 Click Next until the needle assembly is moved to the needle seat 13 Select Back to finish the procedure 14 Close the doors 15 Perform a pressure test ...

Page 206: ...Vespel 1300 bar for 1290 Infinity II Injection Valve OR 1 0101 1416 Rotor seal PEEK for 1260 Infinity Injection Valve Preparations Finish any pending acquisition job and in order to avoid leaks stop the pump running CAUTION Reduced life time of the injection valve Component cleanliness is crucial for the life time of the injection valve Replace the rotor seal in a clean environment CAUTION Removin...

Page 207: ...Maintenance and Repair 9 Exchange the Rotor Seal 1 Open the front door 2 Remove all capillaries from the injection valve with a 1 4 inch wrench 3 Loosen each fixing bolt two turns at a time Remove the bolts from the head 4 Remove the stator head and stator ring ...

Page 208: ... with the appropriate solvent If more stringent cleaning is required use a sonicator Inspect the remaining valve components for contamination Clean them as necessary If the stator head is scratched replace the valve 6 Install the new rotor seal and isolation seal Ensure the metal spring inside the isolation seal faces towards the valve body NOTE Make sure that the rotor sealing surface with its en...

Page 209: ...at the 12 o clock position Ensure the ring sits flat on the valve body 8 Install the stator head Tighten the bolts alternately two turns at a time until the stator head is secure NOTE Do not over tighten the screws The screws hold the assembly together and do not affect the sealing force The sealing force is automatically set as the screws close the stator head against the valve body ...

Page 210: ... 9 Maintenance and Repair Exchange the Rotor Seal 9 Reconnect the capillaries and tubes to the valve ports The correct plumbing is shown below and on the safety cover of the module 10 Slide the waste tube into the waste holder in the leak tray DVWH ROXPQ 3XPS ...

Page 211: ...Agilent InfinityLab LC Series Vialsamplers User Manual 211 Maintenance and Repair 9 Exchange the Rotor Seal 11 Close the doors 12 Perform a pressure test ...

Page 212: ...y 12 cm long 01018 23702 Insert tool Cleaning tissue and appropriate solvent like isopropanol or methanol Parts required p n Description 0905 1503 Metering seal 100 µL 5067 5678 Piston ceramic for 100 µL analytical head 0905 1717 Metering seal 40 µL 5067 5920 Piston ceramic for 40 µL analytical head Preparations Finish any pending acquisition job and in order to avoid leaks stop the pump running N...

Page 213: ...ilent Lab Advisor software select Service Diagnostics Maintenance Positions Change Metering Device click Start and wait until the metering device is in maintenance position 2 Open the front door 3 Disconnect the two capillaries from the metering device 4 Remove the two fixing bolts 5 Pull the metering head assembly away from the sampler 6 Remove the two fixing bolts from the base of the metering h...

Page 214: ...ide of the insert tool to install the new seal Press the seal firmly into position 11 Place the piston guide on top of the seal 12 Reassemble the metering head assembly Carefully insert the piston into the base The closed side of the metering head must be on the same side as the lower one of the two capillary drillings 13 Install the fixing bolts Tighten the bolts securely Tighten the bolts altern...

Page 215: ...wo turns at a time until the metering device is secure 15 Reinstall the capillaries 16 In the Local Controller exit the maintenance mode and select Change metering device function OR In the Tools section of the Agilent Lab Advisor software system screen exit Service Diagnostics Maintenance Positions Change Metering Device click Next and wait until the metering device is in Home position 17 Select ...

Page 216: ... 60010 Gripper assembly Preparations Finish any pending acquisition job 1 In the Local Controller start the maintenance mode and select Change Gripper function OR In the Tools section of the Agilent Lab Advisor software select Service Diagnostics Maintenance Positions Change Gripper click Start and wait until the gripper is in maintenance position 2 Open the front door 3 Identify the slit below th...

Page 217: ...rnal catch and the rotation of the arm will be blocked 6 Hold the paper clip in place press the gripper release button and rotate the gripper arm to the right 7 The gripper arm will come off 8 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left 9 In the Local Controller close Change gripper OR In the Agilent La...

Page 218: ...218 Agilent InfinityLab LC Series Vialsamplers User Manual 9 Maintenance and Repair Exchange the Gripper Arm 11 Close the doors ...

Page 219: ...des the solvent to the peristaltic pump to avoid syphoning effects WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solv...

Page 220: ...e peristaltic pump cartridge 3 Pull the cartridge forward off the motor shaft 4 Disconnect the tubing coupler leading to the wash port and the tubing coupler coming from the solvent bottle 5 Push the new cartridge onto the motor shaft until the clips click into place 6 Connect the wash port tubing to the upper tubing of the new cartridge use sand paper to get a good grip on the tubing ...

Page 221: ...enance and Repair 9 Replace the Peristaltic Pump Cartridge 7 Connect the inlet filter of the solvent bottle Use the syringe to draw enough solvent for completely filling of the peristaltic pump tubing before continuing to prime the peristaltic pump 8 Close the doors ...

Page 222: ...b and in order to avoid leaks stop the pump running and remove the tubings from the solvent bottles If available close the shutoff valves 1 In the Local Controller start the maintenance mode and select Change needle seat function OR In the Agilent Lab Advisor software select Service Diagnostics in the system screen Tools Maintenance Positions Change Needle Loop click Start and wait until the needl...

Page 223: ...injuries Do not touch the tip of the needle 5 Remove the safety cover WARNING Sharp needle Uncovered needles may cause injuries Do not change the needle seat at this point Do not touch the tip of the needle 6 Turn or flap the wash port to the left 7 In Lab Advisor use Next to move the needle in the down position The needle will move slowly downwards and will stop approximately 2 mm above the seat ...

Page 224: ...nge the Wash Port Assembly 8 Disconnect the sample loop from the needle 9 Loosen the fixing screw 10 Lift out the needle 11 Disconnect the wash tubing from the peristaltic pump 12 Release the spring loaded pin 13 Lift the flapper out of the bracket Be careful not to squeeze the wash tubing ...

Page 225: ... assembly by mounting the flapper into the housing of the needle station The pin must be latched into the housing Check the movement of the flapper Check if the tension on the washport spring is large enough that the part well springs back into the original position 16 Install and reconnect the wash tubing to the peristaltic pump 17 Insert the needle 1 Align the needle in the seat then tighten the...

Page 226: ...p to the needle NOTE Do not overtighten the fitting NOTE Incorrect positioning and installation of the needle loop connection can result in damaging and breaking the sample loop 19 In Lab Advisor use Next to lift the needle slowly into the up position 20 Turn or flap the wash port to the right 21 Install the safety cover 1 and fix the screw 2 ...

Page 227: ...227 Maintenance and Repair 9 Exchange the Wash Port Assembly 22 Select Next to move the needle into the needle seat 23 Select Back to finish the procedure 24 Close the doors 25 Purge the wash port Check if solvent will be delivered from the peristaltic pump ...

Page 228: ...cific Information There is no specific information for this module When The installation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be necessary to keep all systems on the same validated revision or if a new module with newer firmware is added to a system or...

Page 229: ...Assembly 40 μL 236 Analytical Head Assembly 100 µL 237 2ps 6pt Injection Valve 1300 bar 238 2ps 6pt Injection Valve 800 bar 239 2ps 6pt Injection Valve 600 bar 240 Integrated Column Compartment 241 Cabinet Kit 242 Sample Thermostat 244 Standard Vialsampler Accessory Kit 246 Multi Draw Kit 248 Additional Part List 249 Column Identifier Kit 2C G4751A 250 This chapter provides information on parts fo...

Page 230: ... Classic G7129 60210 Drawer for 50 x 2 mL Vials Classic Left G7129 60220 Drawer for 50 x 2 mL Vials Classic Right 2 G7129 60084 Analytical Head Assembly 40 µL OR 2 G7129 60082 Analytical Head Assembly 100 µL 3 G1313 60010 Gripper assembly 4 5067 4238 2pos 6port Injection Valve 1300 bar OR 4 5067 4245 2pos 6port Injection Valve 600 bar OR 5067 6689 2pos 6port RC Injection Valve 800 bar 5 5065 4445 ...

Page 231: ...Agilent InfinityLab LC Series Vialsamplers User Manual 231 Parts and Materials for Maintenance 10 Main Assemblies Figure 26 Main assemblies ...

Page 232: ...9 87202 Needle Assembly slotted for high injection volumes G7129 87012 Seat assembly PEEK 0 12 mm G7129 87017 Seat assembly PEEK 0 17 mm 5068 0007 Rotor seal Vespel 1300 bar for 1290 Infinity II Injection Valve 0101 1416 Rotor seal PEEK for 1260 Infinity Injection Valve G7129 60300 Sample Loop 20 µL red coded G7129 60400 Sample Loop 40 µL green coded G7129 60500 Sample Loop 100 µL white coded 5065...

Page 233: ...x 2 0 mL Vials Figure 28 Drawer for 18 x 6 mL Vials Item p n Description G7129 60010 Drawer for 66 x 2 mL Vials G7129 60110 Drawer for 18 x 6 mL Vials G7129 68210 Drawer Kit 100 x 2 mL Vials Classic 1 G7129 60210 Drawer for 50 x 2 mL Vials Classic Left 2 G7129 60220 Drawer for 50 x 2 mL Vials Classic Right G7129 68210 Drawer Kit 100 x 2 mL Vials Classic ...

Page 234: ...ssembly Figure 29 Drawer Kit 100 x 2 mL Vials Classic 1 Drawer for 50 x 2 mL Vials Classic Left 2 Drawer for 50 x 2 mL Vials Classic Right NOTE It is required that all drawers are put into correct positions that is classical drawer 1 50 must be on the left side and classical drawer 51 100 on the right side ...

Page 235: ...ab LC Series Vialsamplers User Manual 235 Parts and Materials for Maintenance 10 External Tray External Tray Figure 30 External tray p n Description G7129 60000 External Tray for 5 x 2 mL Vials G1313 27302 Disposal tube ...

Page 236: ...sembly 40 μL Figure 31 Analytical head assembly 40 µL Item p n Description G7129 60084 Analytical Head Assembly 40 µL 1 G7129 27704 Analytical Head 40 µL 2 0905 1717 Metering seal 40 µL 3 G7129 60184 Seal Support Assembly 40 µL 4 0515 1052 Screw 2 5 mm hex 5 G7129 60006 Adapter Assembly Analytical Head 6 0515 0850 Screws 7 5067 5920 Piston ceramic ...

Page 237: ...bly 100 µL Figure 32 Analytical head assembly 100 µL Item p n Description G7129 60082 Analytical Head Assembly 100 µL 1 G7129 27710 Analytical Head 100 µL 2 0905 1503 Metering seal 100 µL 3 G7129 60182 Seal Support Assembly 100 µL 4 0515 1052 Screw 2 5 mm hex 5 G7129 60006 Adapter Assembly Analytical Head 6 0515 0850 Screws 7 5067 5678 Piston ceramic ...

Page 238: ... Valve 1300 bar 2ps 6pt Injection Valve 1300 bar Figure 33 2ps 6pt Injection Valve 1300 bar Item p n Description 5067 4238 2pos 6port Injection Valve 1300 bar 1 5068 0018 Stator screws 2 5068 0216 Stator Injection Valve 1300 bar 3 5068 0118 Stator ring 4 5068 0007 Rotor seal Vespel 1300 bar 5 1535 4045 Bearing ring ...

Page 239: ...Valve 800 bar 2ps 6pt Injection Valve 800 bar Figure 34 2ps 6pt Injection Valve 600 bar 800 bar Item p n Description 5067 6689 2pos 6port RC Injection Valve 800 bar 1 5068 0018 Stator screws 2 5068 0215 Stator Injection Valve 600 bar 800 bar 3 5068 0118 Stator ring 4 0101 1416 Rotor seal PEEK 5 1535 4045 Bearing ring ...

Page 240: ... Valve 600 bar 2ps 6pt Injection Valve 600 bar Figure 35 2ps 6pt Injection Valve 600 bar 800 bar Item p n Description 5067 4245 2pos 6port Injection Valve 600 bar 1 5068 0018 Stator screws 2 5068 0215 Stator Injection Valve 600 bar 800 bar 3 5068 0118 Stator ring 4 0101 1416 Rotor seal PEEK 5 1535 4045 Bearing ring ...

Page 241: ... Maintenance 10 Integrated Column Compartment Integrated Column Compartment Figure 36 Integrated column compartment p n Description G7130 60030 ICC Column Heater 3 µL volume G7130 60060 ICC Column Heater 6 µL volume 5043 1356 Colum Holder Lamella G7129 60057 Shelf Assembly not shown ...

Page 242: ...rials for Maintenance Cabinet Kit Cabinet Kit Item p n Description G7129 68713 Cabinet Kit 1 Side Cover right 2 Base Cover 3 Side Cover left 4 Top cover 5 G7129 60057 Shelf Assembly G7129 40008 Rail left not shown G7129 40009 Rail right not shown G1313 22406 SCREW FIX not shown ...

Page 243: ...Agilent InfinityLab LC Series Vialsamplers User Manual 243 Parts and Materials for Maintenance 10 Cabinet Kit Figure 37 Cabinet kit ...

Page 244: ...ostat Sample Thermostat The Sample Thermostat Upgrade G4761A contains Item p n Description 1 G7167 60101 InfinityLab Sample Thermostat G4267 81015 Cable Power Sample Cooler not shown G4267 81014 Cable Ribbon Sample Cooler not shown 2110 1519 Fuse 3 50 A125 V not shown 5067 6208 Condensate Drainage Kit not shown ...

Page 245: ...eries Vialsamplers User Manual 245 Parts and Materials for Maintenance 10 Sample Thermostat Figure 38 Sample cooler NOTE The Sample Thermostat contains flammable refrigerant R600a Please check further details for installation ...

Page 246: ... Kit G7129 68705 contains the following parts Item p n Description 1 1 5500 1251 Capillary ST 0 12 mmX 400 mm SL SL 2 3 G1313 44101 Finger Cap 3 1 5043 1013 Tubing Clip 4 1 5500 1254 Tube connector 180 5 1 5500 1155 Tube Connector 90 degree ID 6 4 6 1 5063 6527 Tubing assembly i d 6 mm o d 9 mm 1 2 m to waste 7 1 5181 1519 CAN cable Agilent module to module 1 m 1 5067 5967 Tubing Clip Tube Connect...

Page 247: ...Agilent InfinityLab LC Series Vialsamplers User Manual 247 Parts and Materials for Maintenance 10 Standard Vialsampler Accessory Kit ...

Page 248: ...167 68711 extends Injection volumes up to 3600 μL The Kit contains the following items p n Description G1313 87307 Seat capillary 500 µL 0 5 mm id G1313 87308 Seat capillary 1500 µL 0 9 mm id 5022 6515 Union ZDV NOTE The internal volume of the seat capillary is 400 µL or 1400 µL With the sample loop the maximum draw volume of 3600 µL can be reached ...

Page 249: ...s and Materials for Maintenance 10 Additional Part List Additional Part List p n Description G7129 68001 Insulation Kit G7129 68000 Air guide kit G7129 60014 Calibration Tool including gripper adapter 5023 2540 Adapter ICC Diffusor G7129 40050 Transport Protection ...

Page 250: ...A Kit for G7129A B C Vialsampler integrated column compartment ICC for column identification of up to 2 columns via tags p n Description 5067 5964 Column Identifier Kit Dual 5067 5917 Custum column ID TAG NOTE The InfinityLab Column Identification Tags used in G7116A B and G7129A B C are not compatible with the former Column Identification Tags used in G1316x ...

Page 251: ...User Manual Agilent Technologies 11 Identifying Cables Cable Overview 252 Analog Cables 254 Remote Cables 256 CAN LAN Cables 260 RS 232 Cables 261 USB 262 This chapter provides information on cables used with the Agilent 1200 Infinity Series modules ...

Page 252: ...ription 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs p n Description 5188 8029 ERI to general purpose 5188 8044 Remote Cable ERI ERI 5188 8045 Remote Cable APG ERI 5188 8059 ERI Extension Cable 1 2 m 5061 3378 Remote Cable to 35900 A D converter 01046 60201 Agilent module to general purpose 5188 8057 Fraction Collection ERI remote Y cable p n ...

Page 253: ...hielded 7 m for point to point connection p n Description RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m p n Description 5188 8050 US...

Page 254: ...Cables One end of these cables provides a BNC connector to be connected to Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 35900 A D converters p n 35900 60750 35900 Pin Agilent module Signal Name 1 Not connected 2 Shield Analog 3 Center Analog ...

Page 255: ...les 11 Analog Cables Agilent Module to BNC Connector Agilent Module to General Purpose p n 8120 1840 Pin BNC Pin Agilent module Signal Name Shield Shield Analog Center Center Analog p n 01046 60105 Pin Pin Agilent module Signal Name 1 Not connected 2 Black Analog 3 Red Analog ...

Page 256: ...ion Cable 1 2 m D Sub15 pin male female p n 5188 8029 pin Color code Enhanced Remote Classic Remote Active TTL 1 white IO1 START REQUEST Low 2 brown IO2 STOP Low 3 green IO3 READY High 4 yellow IO4 POWER ON High 5 grey IO5 NOT USED 6 pink IO6 SHUT DOWN Low 7 blue IO7 START Low 8 red IO8 PREPARE Low 9 black 1wire DATA 10 violet DGND 11 grey pink 5V ERI out 12 red blue PGND 13 white green PGND 14 br...

Page 257: ...88 8045 ERI to APG Connector D_Subminiature 15 pin ERI Connector D_Subminiature 9 pin APG p n 5188 8045 Pin ERI Signal Pin APG Active TTL 10 GND 1 1 Start Request 9 Low 2 Stop 8 Low 3 Ready 7 High 5 Power on 6 High 4 Future 5 6 Shut Down 4 Low 7 Start 3 Low 8 Prepare 2 Low Ground Cable Shielding NC ...

Page 258: ...vides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent modules The other end depends on the instrument to be connected to Table 18 5188 8057 ERI to APG and RJ45 p n 5188 8057 Pin ERI Signal Pin APG Active TTL Pin RJ45 10 GND 1 5 1 Start Request 9 High 2 Stop 8 High 3 Ready 7 High 4 Fraction Trigger 5 High 4 5 Power on 6 High 6 Shut Down 4 High 7 Star...

Page 259: ...Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low p n 01046 60201 Wire Color Pin Agilent module Signal Name Active TTL White 1 Digital ground Brown 2 Prepare run Low Gray 3 Start Low Blue 4 Shut down Low Pink 5 Not connected Y...

Page 260: ...e connected to Agilent modules CAN or LAN connectors CAN Cables LAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m p n Description 5023 0203 Cross over network cable shielded 3 m for point to point connection 5023 0202 Twisted pair network cable shielded 7 m for point to point connection ...

Page 261: ...iption RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem Cable with full handshaking where the wiring is made between pins 1 1 2 3 3 2 4 6 5 5 6 4 7 8 8 7 9 9 5181 1561 RS 232 cable 8 m ...

Page 262: ...plers User Manual 11 Identifying Cables USB USB To connect a USB Flash Drive use a USB OTG cable with Mini B plug and A socket p n Description 5188 8050 USB A M USB Mini B 3 m PC Module 5188 8049 USB A F USB Mini B M OTG Module to Flash Drive ...

Page 263: ...ear View of the Module 268 Serial Number Information 268 Interfaces 269 Overview Interfaces 271 ERI Enhanced Remote Interface 274 USB Universal Serial Bus 276 Setting the 6 bit Configuration Switch 277 Special Settings 279 Instrument Layout 281 Early Maintenance Feedback EMF 282 This chapter describes the detector in more detail on hardware and electronics ...

Page 264: ...perties are the complete communication capabilities CAN LAN USB and RS 232 memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN USB and RS 232 memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common...

Page 265: ...number for example 1315B for the G1315B DAD R the firmware revision for example A for G1315B or B for the G1315C DAD VVV is the revision number for example 650 is revision 6 50 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in chapter Maintenance or use the documentation provided with the Firmware Update Tools Figure 39 Firmware Upd...

Page 266: ...r initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in specific control software environments In this case the feature set of the target type is used and the feature set of the original one is lost After re branding e g from G1314B to G1314C the original f...

Page 267: ...module from a computer through a LAN connection This connector is activated and can be configured with the configuration switch With the appropriate software the USB connector may be used to control the module from a computer through a USB connection The power input socket accepts a line voltage of 100 240 VAC 10 with a line frequency of 50 or 60 Hz Maximum power consumption varies by module There...

Page 268: ...on The serial number information on the instrument labels provide the following information RQILJXUDWLRQ VZLWFK 3RZHU VRFNHW 1 5 1 86 0LQL 3RUW CCXZZ00000 Format CC Country of manufacturing DE Germany JP Japan CN China X Alphabetic character A Z used by manufacturing ZZ Alpha numeric code 0 9 A Z where each combination unambiguously denotes a module there can be more than one code for the same mod...

Page 269: ...S 232 Analog APG A ERI E Special Pumps G7104A C 2 No Yes Yes 1 A G7110B 2 Yes Yes No No E G7111A B G5654A 2 Yes Yes No No E G7112B 2 Yes Yes No No E G7120A 2 No Yes Yes 1 A G7161A B 2 Yes Yes No No E Samplers G7129A B C 2 Yes Yes No No E G7167B C G5667A 2 Yes Yes No No E G7157A 2 Yes Yes No No E Detectors G7114A B 2 Yes Yes No 1 E G7115A 2 Yes Yes No 1 E G7117A B C 2 Yes Yes No 1 E G7121A B 2 Yes ...

Page 270: ...o No Requires a host module with on board LAN with minimum FW B 06 40 or C 06 40 or with additional G1369C LAN Card G1364E F G5664B 2 Yes Yes No No E THERMOSTAT for G1330B Others G7116A B 2 No No No No No Requires a HOST module via CAN G7122A No No No Yes No A G7170B 2 No No No No No Requires a host module with on board LAN with minimum FW B 06 40 or C 06 40 or with additional G1369C LAN Card Tabl...

Page 271: ...on board LAN nor an interface slot for a LAN card e g G1170A Valve Drive or G4227A Flexible Cube These are hosted modules and require a Host module with firmware B 06 40 or later or with additional G1369C LAN Card USB The USB interface replaces the RS 232 Serial interface in new FUSION generation modules For details on USB refer to USB Universal Serial Bus on page 276 Analog Signal Output The anal...

Page 272: ...the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addit...

Page 273: ...ection Receiver is any module performing run time controlled activities 3 READY H System is ready for next analysis Receiver is any sequence controller 4 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 5 Not used 6 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk ...

Page 274: ...ectronics use ERI This interface is already used in the Agilent Universal Interface Box 2 UIB2 ERI Description The ERI interface contains eight individual programmable input output pins In addition it provides 24 V power and 5 V power and a serial data line to detect and recognize further add ons that could be connected to this interface This way the interface can support various additional device...

Page 275: ...ed 1 Wire Data Future Use This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI device The firmware can detect the connected type of device automatically and update information in the device if required Pin Enhanced Remote 1 IO 1 START REQUEST 2 IO 2 STOP 3 IO 3 READY 4 IO 4 POWER ON 5 IO 5 NOT USED 6 IO 6 SHUT DOWN 7 IO 7 START 8 IO 8 PREPARE 9 1 wire DATA 10...

Page 276: ... Available by firmware command defined turn on off For devices that need higher power Class 0 0 5 A maximum 12 W Class 1 1 0 A maximum 24 W Class 2 2 0 A maximum 48 W Class depends on hosting module s internal power overhead If a connected device requires more power the firmware detects this overcurrent detection and provides the information to the user interface Fuse used for safety protection on...

Page 277: ...onics Switch settings provide configuration parameters for LAN and instrument specific initialization procedures All modules with FUSION electronics Default is ALL switches DOWN best settings Default IP address for LAN 192 168 254 11 For specific LAN modes switches 4 5 must be set as required For boot resident cold start modes switches 1 2 or 6 must be UP Figure 42 Location of Configuration switch...

Page 278: ...M settings are tak en from non volatile memory 0 n a 2 2 not assigned Always keep these switches on position 0 off n a LAN Init Mode n a Use Default IP Address3 3 Default IP Address is 192 168 254 11 0 0 0 0 0 Use Stored IP Address 0 0 0 1 0 Use DHCP to request IP Address4 4 Host Name will be the MAC address 0 0 1 0 0 Test 1 System n a n a n a ColdStart Boot Main System Keep Data 0 0 0 0 0 Boot Re...

Page 279: ... start can be used to bring the module into a defined mode with default parameter settings Boot Main System Revert to Default Data The instrument will boot to main mode and changes to the module s default parameter May be also required to load resident firmware into the module Boot Resident System Revert to Default Data The instrument will boot to resident mode and changes to the module s default ...

Page 280: ...tion Switch Table 22 Boot Resident Forced Coldstart SW1 SW2 SW3 SW4 SW5 SW6 Init Mode 1 0 0 0 0 0 Boot Main System Keep Data 1 1 0 0 0 0 Boot Resident System Keep Data 1 0 0 0 0 1 Boot Main System Revert to Default Data 1 1 0 0 0 1 Boot Resident System Revert to Default Data Note The setting 0 down is essential ...

Page 281: ... pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations th...

Page 282: ...he user interface when the limit is exceeded Some counters can be reset to zero after the required maintenance procedure Using the EMF Counters The user settable EMF limits for the EMF Counters enable the early maintenance feedback to be adapted to specific user requirements The useful maintenance cycle is dependent on the requirements for use Therefore the definition of the maximum limits need to...

Page 283: ...ation Switches 286 Initialization Mode Selection 287 Dynamic Host Configuration Protocol DHCP 289 General Information DHCP 289 Setup DHCP 290 Manual Configuration 292 With Telnet 293 PC and Agilent ChemStation Setup 297 PC Setup for Local Configuration 297 Agilent ChemStation Setup 300 This chapter provides information on connecting the module to the Agilent ChemStation PC ...

Page 284: ...dress can be found on a label at the rear of the module underneath the configuration switch see Figure 44 on page 284 Figure 43 MAC Label 2 Connect the instrument s LAN interface see Figure 44 on page 284 to the PC network card using a crossover network cable point to point or a hub or switch using a standard LAN cable Figure 44 Location of LAN interfaces and MAC label NOTE This chapter is generic...

Page 285: ...based DHCP Server using the so called Dynamic Host Configuration Protocol This mode requires a LAN onboard Module or a G1369C LAN Interface card see Setup DHCP on page 290 by manually setting the parameters using Telnet by manually setting the parameters using the Local Controller The LAN interface differentiates between several initialization modes The initialization mode short form init mode def...

Page 286: ...uration Switches The configuration switch can be accessed at the rear of the module Figure 45 Location of Configuration switch example shows a G7114A B VWD The module is shipped with all switches set to OFF as shown above RQILJXUDWLRQ VZLWFK NOTE To perform any LAN configuration SW1 and SW2 must be set to OFF ...

Page 287: ... mode Using Stored is selected the parameters are taken from the non volatile memory of the module The TCP IP connection will be established using these parameters The parameters were configured previously by one of the described methods Figure 46 Using Stored Principle Table 23 Initialization Mode Switches SW1 SW2 SW3 SW4 SW5 SW6 Init Mode 0 0 0 0 0 0 Use Default IP Address 0 0 0 0 1 0 Use Stored...

Page 288: ...e may then close the session select the initialization mode Using Stored power on again and establish the TCP IP connection using the new parameters When the module is wired to the PC directly e g using a cross over cable or a local hub separated from the local area network the user may simply keep the default parameters to establish the TCP IP connection FWLYH 3DUDPHWHU HIDXOW 3DUDPHWHU NOTE Usin...

Page 289: ...eters obtained become the active parameters immediately They are not stored to the non volatile memory of the card Besides requesting the network parameters the card also submits its hostname to the DHCP Server The hostname equals the MAC address of the card e g 0030d3177321 It is the DHCP server s responsibility to forward the hostname address information to the Domain Name Server The card does n...

Page 290: ...Card G1369C and B firmware B 06 40 or above or modules with D firmware All modules should use latest firmware from the same set 1 Note the MAC address of the LAN interface provided with G1369C LAN Interface Card or Main Board This MAC address is on a label on the card or at the rear of the main board e g 0030d3177321 On the Local Controller the MAC address can be found under Details in the LAN sec...

Page 291: ... g OpenLAB CDS ChemStation Edition Lab Advisor Firmware Update Tool and use MAC address as host name e g 0030d3177321 The LC system should become visible in the control software see Note in section General Information DHCP on page 289 Table 25 G1369C LAN Interface Card configuration switch on the card SW 4 SW 5 SW 6 SW 7 SW 8 Initialization Mode ON OFF OFF OFF OFF DHCP Table 26 LC Modules with 8 b...

Page 292: ...n volatile memory of the module It never affects the currently active parameters Therefore manual configuration can be done at any time A power cycle is mandatory to make the stored parameters become the active parameters given that the initialization mode selection switches are allowing it Figure 50 Manual Configuration Principle 7 1 7 6HVVLRQ RQWURO 0RGXOH 6WRUHG 3DUDPHWHU 1RQ 9RODWLOH 5 0 ...

Page 293: ...cking on Windows START button and select Run Type cmd and press OK 2 Type the following at the system DOS prompt c telnet IP address or c telnet host name Figure 51 Telnet Starting a session where IP address may be the assigned address from a Bootp cycle a configuration session with the Handheld Controller or the default IP address see Configuration Switches on page 286 When the connection was est...

Page 294: ...hen press Enter where parameter refers to the configuration parameter you are defining and value refers to the definitions you are assigning to that parameter Each parameter entry is followed by a carriage return Table 27 Telnet Commands Value Description displays syntax and descriptions of commands displays current LAN settings ip x x x x sets new ip address sm x x x x sets new subnet mask gw x x...

Page 295: ...red mode information about the LAN interface MAC address initialization mode Initialization mode is Using Stored active TCP IP settings TCP IP status here ready connected to PC with controller software e g Agilent ChemStation here not connected Figure 55 Telnet Change IP settings change of IP setting to Initialization mode is Using Stored active TCP IP settings stored TCP IP settings in non volati...

Page 296: ...typing the configuration parameters type exit and press Enter to exit with storing parameters Figure 56 Closing the Telnet Session NOTE If the Initialization Mode Switch is changed now to Using Stored mode the instrument will take the stored settings when the module is re booted In the example above it would be 192 168 254 12 ...

Page 297: ...gilent ChemStation Setup PC Setup for Local Configuration This procedure describes the change of the TCP IP settings on your PC to match the module s default parameters in a local configuration see Table 24 on page 288 1 Open the Local Area Connection Properties and select Internet Protocol TCP IP Then click on Properties ...

Page 298: ...8 Agilent InfinityLab LC Series Vialsamplers User Manual 13 LAN Configuration PC and Agilent ChemStation Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration ...

Page 299: ...LC Series Vialsamplers User Manual 299 LAN Configuration 13 PC and Agilent ChemStation Setup 3 We will use the direct LAN access via Cross over LAN cable with the module s IP address 4 Click on OK to save the configuration ...

Page 300: ...etup for the 1290 Infinity system using the 1290 Infinity DAD G4212A as the interfacing module The setup works in the same way for all other systems 1 Open the ChemStation Configuration Editor 2 Select from the menu Configure Instruments 3 Select Modular 3D LC System 4 Give the Instrument a name NOTE The LAN must be connected to detector due to high data load on communication to Control Software ...

Page 301: ...Agilent InfinityLab LC Series Vialsamplers User Manual 301 LAN Configuration 13 PC and Agilent ChemStation Setup 5 Click on OK 6 Select LC System Access Access Point and click on Add ...

Page 302: ...n OK The Configuration Editor shows now the new instrument 8 If required change under Configure Path the folder locations 9 Save the current configuration via File Save 10 Exit the Configuration Editor 11 Start the Agilent ChemStation During first startup or when the system configuration has changed a notification shows up ...

Page 303: ...ould be configured You may select the module manually from the list We use the Auto Configuration mode Click on Yes 13 Enter the IP address or the Hostname of the module with the LAN access 14 Click on OK The selected module is shown now in the right window with serial number In addition all other modules connected via CAN to the detector are shown as well ...

Page 304: ...and Agilent ChemStation Setup 15 Click on OK to continue the ChemStation loading 16 You may see the details of the module by selecting the module and clicking on Configure Under Connection Settings you may change the IP Hostname of the module may require a re start of the ChemStation ...

Page 305: ... User Manual 305 LAN Configuration 13 PC and Agilent ChemStation Setup After successful load of the ChemStation you should see the module s as active item in the graphical user interface GUI Figure 57 Screen After Successful Load of ChemStation ...

Page 306: ...306 Agilent InfinityLab LC Series Vialsamplers User Manual 13 LAN Configuration PC and Agilent ChemStation Setup ...

Page 307: ...Instrument 309 Do Not Operate in an Explosive Atmosphere 310 Do Not Remove the Instrument Cover 310 Do Not Modify the Instrument 310 In Case of Damage 310 Solvents 311 Symbols 312 Waste Electrical and Electronic Equipment WEE Directive 314 Refrigerant 315 Radio Interference 318 Sound Emission 319 Solvent Information 320 Agilent Technologies on Internet 321 This chapter provides addition informatio...

Page 308: ...ssumes no liability for the customer s failure to comply with these requirements Safety Standards This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards General Do not use this product in any manner not specified by the manufacturer The protective features of this product may be impaired if...

Page 309: ... compliance with safety or EMC regulations Make all connections to the unit before applying power NOTE Note the instrument s external markings described under Symbols on page 312 WARNING Missing electrical ground Electrical shock If your product is provided with a grounding type power plug the instrument chassis and cover must be connected to an electrical ground to minimize shock hazard The groun...

Page 310: ...G Presence of flammable gases or fumes Explosion hazard Do not operate the instrument in the presence of flammable gases or fumes WARNING Instrument covers removed Electrical shock Do Not Remove the Instrument Cover Only Agilent authorized personnel are allowed to remove instrument covers Always disconnect the power cables and any external circuits before removing the instrument cover WARNING Dama...

Page 311: ...w the boiling point of the solvent used Do not operate the instrument in an explosive atmosphere Do not use solvents of ignition Class IIC according IEC 60079 20 1 for example carbon disulfide Reduce the volume of substances to the minimum required for the analysis Never exceed the maximum permissible volume of solvents 8 L in the solvent cabinet Do not use bottles that exceed the maximum permissi...

Page 312: ...uorinated greenhouse gas refrigerant according to the Kyoto protocol For specifications of refrigerant charge capacity carbon dioxide equivalent CDE and global warming potential GWP see instrument label Flammable Material For Sample Thermostat which uses flammable refrigerant consult Agilent Information Center User Manual before attempting to install or service this equipment All safety precaution...

Page 313: ... damage TVs and laptops computer hard drives credit and ATM cards data storage media mechanical watches hearing aids and speakers Keep magnets at least 25 mm away from devices and objects that could be damaged by strong magnetic fields Indicates a pinching or crushing hazard Indicates a piercing or cutting hazard Table 28 Symbols WARNING A WARNING alerts you to situations that could cause physical...

Page 314: ... WEE Directive This product complies with the European WEEE Directive marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste NOTE Do not dispose of in domestic household waste To return unwanted products contact your local Agilent office or see http www agilent com for more information ...

Page 315: ...ilent Infinity II Sample Cooler Table 29 Physical properties of refrigerant HFC 134a Molecular weight 102 Critical temperature 101 1 C Critical pressure 40 6 bar Boiling point 26 5 C Table 30 Physical properties of refrigerant R600a isobutane Molecular weight 58 12 Critical temperature 134 98 C Critical pressure 36 6 bar Boiling point 11 7 C ...

Page 316: ...ons After inhalation move the affected person to fresh air keep him warm and allow him to rest If necessary he should be supplied with oxygen If he has stopped breathing or is breathing erratically he should be given artificial respiration In the case of cardiac arrest carry out heart massage Send for a doctor immediately Moreover it must be noted that HFC 134a must always be extracted from the sy...

Page 317: ...CAUTION Risk of fire or explosion Dispose of properly in accordance with federal or local regulations Flammable Refrigerant Used Do not dispose of in domestic household waste To return unwanted products contact your local Agilent office or see http www agilent com for more information ...

Page 318: ...gilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises ...

Page 319: ...Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound Pressure Lp 70 dB A At Operator Position Normal Operation According to ISO 7779 1988 EN 27779 1991 Type Test ...

Page 320: ...horic acid or phosphate buffer which are less corrosive against stainless steel halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3 O2 2COCl2 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol chromatographic grade ethers which can contain peroxides for e...

Page 321: ...s Vialsamplers User Manual 321 Appendix 14 Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com ...

Page 322: ...mn exchange 76 compensation sensor open 170 compensation sensor short 170 condensation 39 configuration switche 286 D defect on arrival 90 DHCP general information 289 setup 290 dimensions 40 doors install 191 191 remove 191 191 draw speed 138 138 DRAW 138 153 drawer 78 drawers exchange 58 E eject speed 138 138 EJECT 138 electrical connections descriptions of 267 electronic waste 314 EMF early mai...

Page 323: ...onfiguration ChemStation 300 PC setup 297 LAN cable 260 configuration switche 286 manual configuration with telnet 293 manual configuration 292 PC and Agilent ChemStation Setup 297 TCP IP parameter configuration 285 using default 288 using stored 287 what you have to do first 284 284 leak sensor open 169 leak sensor short 169 leak sensor incompatibility 32 leak sensor 32 168 line frequency 40 line...

Page 324: ...nge 212 plunger home 153 power consideration 36 power consumption 40 power cords 37 product description G7129A 10 G7129B 12 G7129C 14 R remote ERI 272 remote cables 256 remove doors 191 repairs replacing firmware 228 reset 154 rotor seal exchange 206 RS 232C cable 261 S safety class I 308 safety general information 308 standards 40 symbols 312 sample cooler installation 91 sample drawers 78 sample...

Page 325: ...pass 153 valve capillaries 117 valve mainpass 154 vial positions 78 vial to seat 153 vial to tray 154 vials screw top 82 snap top 82 top crimp 81 viscous samples 138 138 voltage range 40 W waste electrical and electronic equipment 314 WEEE directive 314 weight 40 X X axis 26 Z Z axis 26 ...

Page 326: ...ilent 1260 Infinity II Vialsampler G7129A the Agilent 1290 Infinity II Vialsampler G7129B and the Agilent 1260 Infinity II Vialsampler G7129C The manual describes the following Introduction site requirements and specifications using the module optimizing performance troubleshooting and diagnostics error information maintenance parts and materials hardware information LAN configuration safety and r...

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