Agilent Technologies 1260 Infinity G1315C, User Manual

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156 Agilent 1260 Infinity DAD and MWD User Manual
8
Test Functions
Cell Test
Cell Test
The cell test measures the intensity of the deuterium and tungsten lamps over
the full wavelength range (190 – 950 nm), once with the flow cell installed, and
once with the flow cell removed. The resulting intensity ratio is a measure of
the amount of light absorbed by the flow cell. The test can be used to check for
dirty or contaminated flow cell windows. When the test is started, the 1-nm
slit is moved into the light path automatically, and the gain is set to zero. To
eliminate effects due to absorbing solvents, the test should be done with water
in the flow cell.
Test Evaluation
Cell Test Evaluation
The Agilent ChemStation calculates the intensity ratio automatically. The
intensity ratio (typically between 0.5 and 0.7 for new standard flow cells and
0.1 to 0.3 for new mico- and high pressure cells) is dependent on the degree of
contamination of the flow cell windows, and on the type of flow cell used.
N O T E
This test should be performed inititially with a new detector/flow cell. The values should
be kept for later reference/comparison.