OPERATION
Illumination and contrasting techniques
Axioskop 2 / Axioskop 2
MOT
3-36
B 40-075 e 06/98
3.3.5
Setting of transmitted-light differential interference contrast (DIC)
(1)
General principle
The transmitted-light DIC technique is an alternative contrasting technique for polarization applications,
permitting high-contrast 3D images of transparent specimen details.
Light which has been linearly polarized by a polarizer is split into two partial beams in a birefringent
prism. These partial beams pass two close-lying specimen areas with different refractive indices and
specimen thickness. This causes path differences in both beams. The beams are then united in a second
birefringent prism and feature the same vibration direction after passing the analyzer. Therefore, both
beams can interfere in the intermediate image, with the path differences being transformed into
different gray values (intensities). A
λ
-compensator (lambda plate) subsequently changes the gray values
to colors.
(2)
Axioskop 2 (manual) and Axioskop 2
MOT
configuration
−
Objectives offered with DIC equipment, e.g. Plan-Neofluar
−
Nosepiece with compartment for DIC slider
−
DIC slider, suitable for the objectives used
−
Condenser with turret disk with DIC prisms (DIC I, DIC II, DIC III)
−
Polarizer, e.g. SENARMONT polarizer
☞
In the case of the achromatic-aplanatic universal condensers 0.9 H D Ph DIC, 445439 (or
445440
MOT
) the DIC prisms in the turret disk are already combined with a polarizer. When
other condensers are used, a separate polarizer is therefore required, e.g. the SENARMONT
polarizer.
−
DIC analyzer module in reflector turret
−
Rotary mechanical stage should be preferred
(3)
Setting of transmitted-light DIC on Axioskop 2 (manual) and Axioskop 2
MOT
•
Swing in the DIC-suitable objective via the nosepiece (3-25/
1
). Push the relevant DIC slider (3-25/
3
) in
the compartment on the nosepiece until it click-stops.
•
Swing in the DIC analyzer module on the reflector turret (3-25/
2
).
•
Swing in the suitable DIC prism I, II or III (digit on condenser turret disk). Nothing else is to be done if
this is the condenser with integrated polarization filter. In case of the version without polarization
filter, the SENARMONT polarizer must also be swung in.
Содержание Axioskop 2
Страница 1: ...Axioskop 2 Routine Microscope Operating Manual...
Страница 14: ...INTRODUCTION Axioskop 2 Axioskop 2 MOT 0 14 B 40 075 e 06 98...
Страница 120: ...OPERATION Axioskop 2 Axioskop 2 MOT 3 54 B 40 075 e 06 98...
Страница 122: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 2 B 40 075 e 06 98...
Страница 132: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 12 B 40 075 e 06 98...
Страница 134: ...ANNEX Axioskop 2 Axioskop 2 MOT A 2 B 40 075 e 06 98...