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OPERATION

Axioskop 2 / Axioskop 2 

MOT

Illumination and contrasting techniques

B 40-075 e    06/98

3-35

3.3.4.3 

Circular polarization contrast

(1)

Application

Unlike polarization contrast, circular polarization contrast does not feature any dark positions which
depend on the turning angle (azimuth) of the specimen in relation to the polarizer or analyzer. All
transparent specimens feature their specific interference colors in optical anisotropy.

(2)

Axioskop 2 (manual) and Axioskop 2 

MOT

 configuration

− 

Objectives suitable for polarization

− 

Rotary mechanical stage 75x50/240° R

− 

Circular polarizer D (polarizers must not be integrated in the condenser), including the relevant 

λ

/4-

plate

− 

Analyzer module D in reflector turret

(3)

Setting of Axioskop 2 (manual)
and Axioskop 2 

MOT

• 

Set the microscope as for transmitted-light
brightfield according to KÖHLER (see section
3.3.1 (3)).

• 

Center the rotary stage (3-24/

3

) (see section

2.2.8 (2))  and  then  remove  specimen  from  the
stage.

• 

Swing circular polarizer (3-24/

2

) in the beam

path.

• 

Swing analyzer module D on reflector turret
(3-24/

4

). The field of view now appears dark on

account of the crossed polarizers.

• 

Push the upper 

λ

/4-plate (3-24/

1

) into the

compartment for compensators above the
nosepiece until stop. The previously dark field
of view becomes bright again.

• 

Turn the 

λ

/4-plate of the circular polarizer

(3-24/

2

) until optimum darkness of the field of

view is achieved. Close the aperture diaphragm
by approx. 2/3 of its diameter to increase the
dark position.

• 

Position the specimen to be examined on the
mechanical stage again.

Fig. 3-24

Components for transmitted-light
polarization on the Axioskop 2

Содержание Axioskop 2

Страница 1: ...Axioskop 2 Routine Microscope Operating Manual...

Страница 2: ...nual is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the software package and the utilization and communication of its contents are not...

Страница 3: ...iew of Axioskop 2 0 12 Overall view of Axioskop 2 MOT 0 13 CHAPTER 1 INSTRUMENT DESCRIPTION 1 2 1 1 Name and intended application 1 2 1 2 Instrument description and main features 1 2 1 3 Axioskop 2 ma...

Страница 4: ...s 2 15 2 2 1 Changing the condenser 2 15 2 2 2 Changing the phase stop for phase contrast or darkfield in the universal condenser 2 16 2 2 3 Changing the DIC prism in the universal condenser 2 17 2 2...

Страница 5: ...ansmitted light darkfield 3 27 3 3 3 Setting of transmitted light phase contrast 3 29 3 3 4 Setting of transmitted light polarization 3 31 3 3 5 Setting of transmitted light differential interference...

Страница 6: ...maintenance 4 4 4 2 1 Performing checks 4 4 4 2 2 Changing the fuses 4 4 4 2 3 Check instrument voltage and change it if required 4 5 4 3 Troubleshooting 4 6 4 4 Spares consumables and tools 4 10 4 5...

Страница 7: ...uched by the condenser 5 Close luminous field diaphragm until it is visible in the field of view even if not in focus Fig 0 1 A 6 Lower universal condenser 0 9 until the edge of the luminous field dia...

Страница 8: ...et the requirements of the EC directive 89 336 EC and the EMC legislation of November 9th 1992 This operation manual includes information and warnings which must be observed by the user The following...

Страница 9: ...ertent operation Contact a Zeiss service agency or Carl Zeiss microscopy service for the repair of the instrument The power unit which is integrated in the stand of the microscope permits the use of l...

Страница 10: ...nce filters are used the filter protecting from heat emitted by the microscope illuminator must not be removed since fluorescence filters are sensitive to heat and their performance might therefore be...

Страница 11: ...is provided for defects caused by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any other med...

Страница 12: ...Axioskop 2 Axioskop 2 Axioskop 2 MOT 0 12 B 40 075 e 06 98 Overall view of the Axioskop 2 1 Eyepieces 2 Binocular tube 3 Stand 4 Incident light illuminator 5 Transmitted light illuminator 6 Condenser...

Страница 13: ...f the Axioskop 2 MOT B 40 075 e 06 98 0 13 Overall view of the Axioskop 2 MOT 1 Eyepieces 2 Binocular ergotube 3 Stand 4 Incident light illuminator 5 FL excitation filter wheel 6 Transmitted light ill...

Страница 14: ...INTRODUCTION Axioskop 2 Axioskop 2 MOT 0 14 B 40 075 e 06 98...

Страница 15: ...iption and main features 1 2 1 3 Axioskop 2 manual microscope configurations and modules 1 4 1 4 Axioskop 2 MOT microscope configurations and modules 1 8 1 5 Objectives 1 12 1 6 Eyepieces 1 14 1 7 Sta...

Страница 16: ...hnical performance Thanks to the modular design existing microscope modules of the Axioskop 2 such as tubes intermediate tubes and condensers are easy to attach The Axioskop 2 is offered as a manual a...

Страница 17: ...fluar Plan Apochromat Binocular tubes and phototubes with fixed and variable viewing angle 10x focusing eyepieces with field number 23 suitable for spectacle wearers High performance epi fluorescence...

Страница 18: ...op 2 microscope configurations Axioskop 2 Axioskop 2 MOT 1 4 B 40 075 e 06 98 1 3 Axioskop 2 manual microscope configurations and modules Fig 1 1 Axioskop 2 manual microscope configurations 1 0 2 0 3...

Страница 19: ...tube 30 23 4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen lamp 7 FL filter slider 8 Objective 9 DIC slider 10 Compensator 11 Dust cover 12 DIC prism 13 Achromatic aplanatic uni...

Страница 20: ...Neofluar 2 5 10x Ph 1 20x Ph 2 40x Plan Apochromat 63x and Plan Neofluar 100x Modules The Axioskop 2 routine microscope consists of the following modules Axioskop 2 stand with 4x reflector turret Har...

Страница 21: ...h ceramic coating and fixed coaxial drive 130 mm 453505 9901 453502 9905 453523 Specimen holder with spring clip R 453533 Technique modules FL reflector module e g with filter sets for epi fluorescenc...

Страница 22: ...NT DESCRIPTION Axioskop 2 MOT microscope configurations Axioskop 2 Axioskop 2 MOT 1 8 B 40 075 e 06 98 1 4 Axioskop 2 MOT microscope configurations and modules Fig 1 2 Axioskop 2 MOT microscope config...

Страница 23: ...transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen illuminator 7 FL filter slider 8 Objective 9 DIC slider 10 Compensator 11 Dust cover 12 DIC prism 13 Achromatic aplanatic universal condenser...

Страница 24: ...nosepiece H DIC W 0 8 stabilized 12 V DC 100 W power unit MOT transmitted light equipment stage and condenser carrier FL incident light equipment and HBO XBO adjusting aid 452311 Eyepieces W PL 10x 2...

Страница 25: ...filter set 01 UV excitation H 365 filter set 09 blue excitation 450 490 filter set 15 green excitation H 546 DIC FL analyzer Analyzer module D Optovar module 1 25x Optovar module 1 6x Optovar module...

Страница 26: ...green light blue dark blue white Magnification factor 1 25x 2 5x 4x 5x 6 3x 10x 16x 20x 25x 32x 40x 50x 63x 100x 150x The objective magnification multiplied with the eyepiece magnification minimum 10x...

Страница 27: ...Plan 100x 1 25 Oil Ph 3 0 22 0 17 441081 Achroplan Transmitted light brightfield Achroplan 4x 0 10 11 1 440020 Achroplan 10x 0 25 4 8 440030 Achroplan 20x 0 45 2 07 0 17 440040 Achroplan 40x 0 65 0 5...

Страница 28: ...ss up to the edge of the field of view PL 10x 23 Br foc eyepieces ensure excellent optical correction particularly in combination with the ergotubes If required eyecups for the eyepieces can be ordere...

Страница 29: ...0 001 mm 474066 9901 Net micrometer 12 5x12 5 5 10 d 26 mm area 12 5x12 5 mm divided in fields of 10x10 474068 Photo reticle MC 2 5x d 26 mm for 35 mm photography with an additional magnification of 2...

Страница 30: ...kg Axioskop 2 MOT approx 22 4 kg Ambient conditions Storage and transport in packaging Permissible ambient temperature 10 to 60 C Permissible relative humidity 20 to 85 at 35 C Atmospheric pressure 80...

Страница 31: ...ooms Protection class I Enclosure protection IP 20 Line voltage can be changed between 115 VAC and 230 VAC Line frequency 50 to 60 Hz Power consumption when HBO 103 is used 265 VA Fuses in accordance...

Страница 32: ...ns for brightfield darkfield for phase contrast 1 2 3 for DIC I II III changeable Objective change manual via 5 position nosepiece H DIC W 0 8 Change of technique modules manually via 4 position refle...

Страница 33: ...ns 2 15 2 2 1 Changing the condenser 2 15 2 2 2 Changing the phase stop for phase contrast or darkfield in the universal condenser 2 16 2 2 3 Changing the DIC prism in the universal condenser 2 17 2 2...

Страница 34: ...tage 2 13 Fig 2 22 Axioskop 2 MOT rear 2 14 Fig 2 23 Changing the condenser 2 15 Fig 2 24 Attachment of condenser MOT 2 16 Fig 2 25 Changing the phase stop 2 16 Fig 2 26 Changing the DIC prism 2 17 Fi...

Страница 35: ...ves eyepieces condenser 12 V 100 W illuminator microscope mat and various small parts e g DIC slider spare lamp dust cover tools bag The following components are factory attached to the microscope sta...

Страница 36: ...n the required observation position and tighten the hexagonal screw using the ball headed screwdriver 2 1 3 Insertion of eyepieces and centering telescope Remove both dust covers 2 3 1 and 4 from the...

Страница 37: ...reticle If eyepiece reticles are inserted into the unscrewed mount by the customer attention must be paid to the labelling being visible the right way up after insertion Compensation of ametropia when...

Страница 38: ...Furthermore the binocular component of the ergotubes can be swivelled by 180 2 1 6 Screw in of objectives Move mechanical stage with carrier to the lower stop position Remove dust caps 2 6 2 from the...

Страница 39: ...the locking screw on the underside of the condenser in the direction of the groove 2 7 7 Press dovetail of condenser against the spring mount 2 7 6 of the condenser carrier until the condenser can be...

Страница 40: ...r The HBO 100 W transformer must be connected to the line via its line socket 2 10 1 Before switching on the HBO 100 W transformer on the instrument rear check whether the correct instrument voltage h...

Страница 41: ...inator a Coarse adjustment Loosen clamping screw 2 12 2 and remove the halogen illuminator from the microscope stand Switch on microscope as described under 2 1 9 Direct the light beam against a proje...

Страница 42: ...filters 2 1 11 HBO 103 illuminator 1 Attach HBO 103 W 2 mercury vapor short arc lamp The HBO 103 illuminator and the HBO 103 W 2 mercury vapor short arc lamp are supplied in separate packages for saf...

Страница 43: ...HBO 103 illuminator from the microscope stand by loosening clamping screw 2 14 2 Switch on HBO 103 on HBO 100 W transformer 2 11 1 and allow it to heat to operating temperature Make sure not to look...

Страница 44: ...cribed in section 2 1 11 2 Pull adjusting aid 2 16 2 out of the incident light tube 2 16 1 The brighter focal spot of the HBO 103 W 2 lamp and its slightly darker reflector image become visible in the...

Страница 45: ...escribed in section 2 1 11 2 Remove one objective from the nosepiece and switch empty opening in function position Place a white sheet of paper on the mechanical stage Fully open the precentered lumin...

Страница 46: ...combined CAN SUB BUS 2 22 1 connectors 2 1 13 Attachment of round cable holder The round cable holder supplied with the mechanical stages is suitable to hold and store the SW 2 screwdriver on the mic...

Страница 47: ...stand of connecting cable and pull off the connecting cable Unscrew both centering screws 2 23 3 until condenser can be easily taken out of its mount Lift available condenser from the orientation gro...

Страница 48: ...r on the condenser underside 2 25 2 loosen both grub screws and remove the cover Position turret disk with phase stop to be changed in the exchange opening and hold it on the knurled ring since no cli...

Страница 49: ...ng and hold it on the knurled ring Unscrew retainer ring using the so called double function tool 2 26 4 Now screw the tool in the prism mount 2 26 3 with the threaded side 2 26 4 and pull out DIC pri...

Страница 50: ...r 2 27 1 to screw stop bolt 2 27 2 into the rear threaded hole of the condenser carrier until stop 2 2 5 Attachment of SENARMONT DIC polarizer Hold polarizer 2 28 6 parallel to the underside of the co...

Страница 51: ...lever and pull the protection cap off Press spring lever briefly downwards again to center the lamp Attach lamp housing again and tighten clamping screw 2 29 5 2 2 7 Changing the HBO 103 W 2 mercury...

Страница 52: ...ing knob 2 32 1 to bring collector to the position at the very front in the direction of the light Use ball headed screwdriver to loosen clamping screw for lamp housing 2 32 11 and carefully remove la...

Страница 53: ...2 lamp engages into the base Carefully and safely apply 17 mm fork wrench 2 32 6 on the hexagonal nut 2 32 7 and tighten it Make absolutely sure that the fork wrench does not slide off to avoid damag...

Страница 54: ...ow and screw them in the stage underside use the shorter screws on the front Orient stage in x y direction and tighten the fixation screws 2 Rotary mechanical stage 75x50 240 R a Removing the stage Lo...

Страница 55: ...sing one SW 1 5 Allen screwdriver 2 35 3 each to move the specimen detail by half the arrow length in the direction of the crossline center Check whether specimen detail moves when the stage is rotate...

Страница 56: ...e drilled hole of drive 2 37 2 Turn drive 2 37 1 to the right or left until the screwdriver clickstops in one of the holes of the inside nut Hold drive for y movement 2 37 1 and use inserted screwdriv...

Страница 57: ...stage and tighten two countersunk screws 2 2 9 Changing the binocular tube Use SW 3 ball headed screwdriver to loosen clamping screw 2 39 3 and remove available tube in an upward direction Place dust...

Страница 58: ...intermediate tube with height adjustment 2 40 5 Insert dovetail of intermediate tube in the stand opening 2 40 8 and tighten clamping screw 2 40 7 only slightly Align intermediate tube with the outer...

Страница 59: ...Insert dovetail of the adapter for the Axioskop 2 2 41 3 in the stand opening 2 41 6 on the underside and slightly tighten clamping screw 2 41 5 Insert fixation screw 2 41 2 in countersunk hole of th...

Страница 60: ...ments of the cover at an angle and then fix the cover to the stand housing via the lower holding elements 2 2 13 Changing the filter set in the FL reflector module The filter sets for the FL reflector...

Страница 61: ...reflector module 2 44 2 and 5 and carefully remove the beam splitter Insert spring frame 2 44 4 in the mount of the lower part 2 44 5 Carefully place new beam splitter with its reflecting side pointi...

Страница 62: ...45 12 to prevent the coobservation equipment from shifting Unscrew the tube lenses from the two binocular tubes using the cover of one of the storage cases and put them in the cases Remove upper dust...

Страница 63: ...r 2 users 8 1 Binocular ergotube 2 Binocular tube 3 Dust cap 4 Rear dovetail 5 Socket for power supply 6 Heat protection shield 7 Fixation screw 8 Clamping screw 9 Support point stand 10 Power unit 11...

Страница 64: ...e the protection caps from the tube ends Each pin must engage in the corresponding opening then tighten the captive coupling nuts Attach and clamp two binocular tubes for the multi observation equipme...

Страница 65: ...e user The multi observation equipment with light pointer permits attachment of 2 5 9 and more observation devices 1 Clamping screw 6 Coobservation tube 11 End connector R 2 Clamping screw 7 End conne...

Страница 66: ...e stand and tighten clamping screw 2 47 10 only slightly at first Centering is performed using the dovetail Insert second clamping screw with washer 2 47 3 in the drilled hole provided on the rear of...

Страница 67: ...of transmitted light darkfield 3 27 3 3 3 Setting of transmitted light phase contrast 3 29 3 3 4 Setting of transmitted light polarization 3 31 3 3 5 Setting of transmitted light differential interfer...

Страница 68: ...p on the universal condenser 3 28 Fig 3 19 Centering of phase stop on universal condenser 3 30 Fig 3 20 Centering of phase stop bright in condenser to phase ring dark in objective 3 30 Fig 3 21 Compon...

Страница 69: ...and in a version with motorized coded components In the Operation chapter the manual setting and operation functions are explained first and the motorized coded functions are explained afterwards whe...

Страница 70: ...N Operation and function controls Axioskop 2 Axioskop 2 MOT 3 4 B 40 075 e 06 98 3 1 1 Operation and function controls of the Axioskop 2 manual Fig 3 1 Operation and function controls of the Axioskop...

Страница 71: ...Color temperature key 3200 K 13 Focusing drive coarse adjustment on both sides 14 Focusing drive fine adjustment on both sides 15 Lever to swing in out the diffusion disk 16 Drive for movement of the...

Страница 72: ...d diaphragm diameter see section 3 3 6 Pushrod pushed in fully open Pushrod pulled out closed Compartment for filter slider For 3 position or 6 position FL filter slider with 18 mm filter diameter Cen...

Страница 73: ...on disk 3 1 15 is used to achieve homogeneous illumination though at a slight loss of light Lever in front position diffusion disk swung out Lever in rear position diffusion disk swung in Drive for mo...

Страница 74: ...heel 3 2 4 swings the brightfield insert and the contrast stops in the beam path The abbreviation of the set turret position e g D is displayed in one of the windows 3 2 5 Slider 3 2 3 opens and close...

Страница 75: ...ue modules by turning the knurled ring of the reflector turret Luminous field diaphragm Wheel 3 4 2 for the continuous setting of the luminous field diaphragm transmitted light Additional mount 3 4 1...

Страница 76: ...eration and function controls Axioskop 2 Axioskop 2 MOT 3 10 B 40 075 e 06 98 3 1 2 Overview of operation and function controls of the Axioskop 2 MOT Fig 3 5 Operation and function controls of the Axi...

Страница 77: ...Close the filter housing and tighten the knurled screw LED lamp voltage array Indicates the present operating voltage of the halogen illuminator in 10 steps 3 12 V In the programming mode it indicate...

Страница 78: ...ture diaphragm is opened until stop as long as the key remains pressed Key close aperture diaphragm 3 6 2 aperture diaphragm is closed until stop as long as the key remains pressed Key front lens on o...

Страница 79: ...y is activated the stage is moved downwards out of the focus position by approx 6 mm The current focus position is stored The specimen can be changed When the stage is in the low position the motorize...

Страница 80: ...ted the transmitted light is switched on or off Transmitted light is always available when the microscope is switched on Set key Stores illumination and condenser settings and is used for programming...

Страница 81: ...to the attached camera via a pushrod Pushrod 3 9 2 pushed in 100 of the light for the eyepieces Pushrod 3 9 2 pulled out 100 of the light for the camera To set the most convenient viewing angle swivel...

Страница 82: ...or in a combination When several filters are combined the transmission value is calculated as the product of the individual transmission values in percent Filter slider The 3 position or 6 position F...

Страница 83: ...ly from 60 to 110 mm This intermediate tube can only be used in combination with the binocular ergotube 6 25 23 or the binocular ergo phototube 6 25 23 100 100 Loosen clamping lever 3 12 2 of the tube...

Страница 84: ...of all the connected components During the system check the pilot lamp on the stand lights up in orange After approx 3 seconds the microscope is ready for operation and is in the so called automatic m...

Страница 85: ...is heard Settings Light intensity depending on objective nosepiece and technique module reflector turret Condenser turret position depending on objective nosepiece and technique module reflector turre...

Страница 86: ...Switching of condenser turret Folding in out of front lens Transmitted light on off Opening closing of aperture diaphragm transmitted light The values for illumination intensity and the condenser func...

Страница 87: ...e parameters for stage focusing are set individually the focus positions of the various objectives parfocality and the focusing speeds are matched to the appropriate objective magnification This is do...

Страница 88: ...a the two keys for fast stage lowering lifting 3 13 2 Key higher speed Key lower speed The focusing speed can be matched to the individual requirements by switching between coarse and fine adjustment...

Страница 89: ...onfirmed by a beep when the Set key is released The pilot lamp lights up in green again If several reflector positions are to be used e g different reflector modules for incident light or Optovar modu...

Страница 90: ...ing to K HLER on the manual Axioskop 2 2 Transmitted light brightfield equipment of the manual Axioskop 2 Each manual Axioskop 2 configuration permits performance of the transmitted light brightfield...

Страница 91: ...ube and look into the tube with your naked eye Use sliding knob 3 16 5 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupils 3 16 E In most applications this ape...

Страница 92: ...focus positions and focus speeds of the used objectives has been finished The instrument is switched on and is in the automatic mode Illumination intensity condenser settings position of the front len...

Страница 93: ...ettings on the manual Axioskop 2 and Axioskop 2 MOT Setting of K HLER illumination is identical to transmitted light brightfield except that the highest aperture objective must be used instead of the...

Страница 94: ...uminous field diaphragm image will be almost perfectly in focus Finally match the diameter of the luminous field diaphragm to the size of the field of view Darkfield microscopy requires specimens to b...

Страница 95: ...ptical channel and are influenced by the in phase refractive index and the thickness differences in the specimen In the intermediate image plane the differently influenced partial beams interfere and...

Страница 96: ...t perfect 3 20 A the two SW 1 5 screwdrivers 3 19 1 and 4 must be used on the two centering screws 3 19 2 and 3 to recenter the bright phase stop until complete congruence with the dark phase ring is...

Страница 97: ...MOT configuration Objectives suitable for polarization Rotary mechanical stage 75x50 240 R SENARMONT DIC polarizer or polarizer D rotary or fixed unless a polarizer is integrated in the condenser Ana...

Страница 98: ...fixed unless a condenser with integrated polarizer is used Analyzer module D in reflector turret Adjusting specimen for polarization microscopy 453679 3 Setting of Axioskop 2 manual and Axioskop 2 MO...

Страница 99: ...vibration direction n defined to be oriented in NE SW direction The specimen changes its color when the compensator is pushed in The type of the color change depends on the orientation of the specimen...

Страница 100: ...examined is parallel to the largest vibration direction of the compensator i e in NE SW direction the path difference of the specimen e g grayish white 150 nm and the path difference of the compensato...

Страница 101: ...et 3 Setting of Axioskop 2 manual and Axioskop 2 MOT Set the microscope as for transmitted light brightfield according to K HLER see section 3 3 1 3 Center the rotary stage 3 24 3 see section 2 2 8 2...

Страница 102: ...manual and Axioskop 2 MOT configuration Objectives offered with DIC equipment e g Plan Neofluar Nosepiece with compartment for DIC slider DIC slider suitable for the objectives used Condenser with tur...

Страница 103: ...izer via the lever in the lower part of the SENARMONT polarizer 3 25 7 The 4 plate above it must always be switched on for SENARMONT contrasting to function If the DIC slider was moved to the center p...

Страница 104: ...Axioskop 2 MOT 3 38 B 40 075 e 06 98 1 Objective in nosepiece 2 Reflector turret 3 DIC slider 4 Knurled screw 5 compensator 6 Sliding knob for aperture diaphragm 7 SENARMONT polarizer Fig 3 25 Compone...

Страница 105: ...L reflector module and shutter module A in reflector turret HBO 103 or HBO 50 mercury vapor short arc lamp for incident light illumination HAL 100 halogen illuminator for transmitted light illuminatio...

Страница 106: ...nblocked Insert eyepiece in tube again and close luminous field diaphragm 3 26 4 until it is visible in the field of view Use the two centering screws 3 26 6 to center the luminous field diaphragm in...

Страница 107: ...s 1 Attachment of a SLR camera e g CONTAX 167 MT Screw T2 adapter 416010 3 27 3 suitable for the used camera on the 2 5x connector for T2 456005 3 27 4 Attach camera 416181 3 27 2 and if required cabl...

Страница 108: ...tion lens 456021 3 28 4 in the microscope camera adapter 3 28 5 Attach MC 80 DX basic body 3 28 2 to the microscope camera adapter until stop align it and tighten clamping ring 3 28 3 anti clockwise A...

Страница 109: ...T2 Adapter for CANON FD bayonet 416004 T2 Adapter for NIKON F bayonet 416009 T2 Adapter for PENTAX KA bayonet 416011 If a PC or notebook with installed software is already connected to the Axioskop 2...

Страница 110: ...ection of 1 chip b w and color CCD cameras and 3 chip color high resolution CCD cameras to the phototube of the Axioskop 2 Axioskop 2 MOT Tube Adapters Cameras 456105 456105 60 C 2 3 1 0x 456107 45610...

Страница 111: ...mm interface to be used with the phototube of the Axioskop 2 Axioskop 2 MOT with 60 mm interface Video adapter Cat No Suitable for Comments 456140 456140 Adapter 60 44 microscopes with 60 mm interfac...

Страница 112: ...30 1 or binocular ergo phototube 6 25 23 to the front tube mount of the microscope Attach binocular tube 3 30 2 or phototube 30 23 to the rear tube mount of the microscope The binocular ergotube 6 25...

Страница 113: ...2 MOT Multi observation equipment B 40 075 e 06 98 3 47 1 Binocular ergotube 2 Binocular tube 3 Basic body 4 Power unit 5 Knob for brightness control 6 Lever for light pointer Fig 3 30 Active coobser...

Страница 114: ...3 31 8 left Binocular tubes 30 23 for further viewing stations 3 31 6 and 7 452927 We would recommend you to have the multi observation equipment assembled by Carl Zeiss service staff since careful o...

Страница 115: ...8 3 49 1 Knob for brightness control 7 Coobservation tube 2 Basic body 8 End connector L 3 Lever for light pointer 9 Power unit 4 Binocular tube 10 End connector R 5 Connecting piece L 11 Connecting p...

Страница 116: ...e of the microscope This enables the user to copy microscope specimens by drawing Furthermore transparent references e g grain size plates reference scales for photomicrography or other self made patt...

Страница 117: ...is exactly that distance in the specimen which complies to one interval of the used crossline micrometer For calibration align the scales of the stage micrometer and the crossline micrometer parallel...

Страница 118: ...the microscope are always possible to be performed if both the lower and upper side of the specimen can be focused This should preferably be performed using a precision Harmonic Drive focusing drive a...

Страница 119: ...ve nM 1 0 The indicated intervals of the mechanical fine drive are 15 0 and 24 5 which means that d 9 5 m with an interval of 1 m The refractive index of the specimen area nP has been assumed as nP 1...

Страница 120: ...OPERATION Axioskop 2 Axioskop 2 MOT 3 54 B 40 075 e 06 98...

Страница 121: ...SHOOTING AND SERVICE 4 3 4 1 Instrument care 4 3 4 2 Instrument maintenance 4 4 4 2 1 Performing checks 4 4 4 2 2 Changing the fuses 4 4 4 2 3 Check instrument voltage and change it if required 4 5 4...

Страница 122: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 2 B 40 075 e 06 98...

Страница 123: ...g solution consisting of 85 isopropanol and 15 medical alcohol benzoline clean glass surface using a slightly moistened wad of cotton by moving in circles starting in the middle Remove dust from optic...

Страница 124: ...he maximum operation time of the halogen and mercury vapor short arc lamps is not exceeded 4 2 2 Changing the fuses Always pull the power plug before changing the fuses The fuse compartment is positio...

Страница 125: ...quency 50 60 Hz Separate setting of the instrument voltage is therefore not required 2 HBO 100 transformer Compare set instrument voltage and line voltage on sliding switch 4 2 1 on the rear of the HB...

Страница 126: ...ube in the correct position end position see page 3 15 visible Nosepiece with objective not in click stop position Switch nosepiece with objective in click stop position Condenser not set correctly Se...

Страница 127: ...r unspecified immersion oil with immersion objectives Use immersion oil 518 N from Carl Zeiss see p 4 10 Air bubbles in the immersion oil Apply new oil to remove the bubbles Immersion oil at the front...

Страница 128: ...a s p 2 5 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set interpupillary distance correctly see p 2 6 Focusing eyepieces are not set correctly Set focus...

Страница 129: ...e selected position illumination is switched off in the interim positions of the nosepiece Correctly click stop nosepiece in the required position Reflector turret is in epi fluorescence positions whe...

Страница 130: ...00 recommended for low brightness techniques to suppress reflected light Dust cover for nosepiece Dust cover for eyepiece tube 462981 0000 000 000000 0168 373 to close instrument openings which are no...

Страница 131: ...MOT may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of your microscope even for a longer period of time we...

Страница 132: ...CARE MAINTENANCE TROUBLESHOOTING AND SERVICE Axioskop 2 Axioskop 2 MOT 4 12 B 40 075 e 06 98...

Страница 133: ...EX Axioskop 2 Axioskop 2 MOT Overview B 40 075 e 06 98 A 1 ANNEX List of abbreviations A 3 List of key words A 5 Certification in accordance with DIN ISO 9001 EN 46001 A 9 EC conformity declaration A...

Страница 134: ...ANNEX Axioskop 2 Axioskop 2 MOT A 2 B 40 075 e 06 98...

Страница 135: ...e of stage height setting focus difference in height measurement DC Direct Current DIC Differential Interference Contrast DIN Deutsches Institut f r Normung German standards association DL transmitted...

Страница 136: ...ractive index of the medium nD refractive index of D line sodium nP refractive index of the specimen Ph phase contras PL flatfield R right drive on the right of the mechanical stage SLR Single Lens Re...

Страница 137: ...15 binocular ergotube 3 14 binocular tube 2 4 3 6 birefringence 3 31 brightfield 3 8 3 20 3 24 3 27 3 29 C care 4 3 changing the fuses 4 4 coarse 3 5 3 11 3 22 coarse drive 3 7 color temperature key 3...

Страница 138: ...ng drive 3 5 3 7 3 9 3 11 3 13 3 26 front lens 1 3 2 7 2 15 3 8 3 12 3 24 3 26 function modes 3 18 3 19 G gear switchover 3 11 3 13 H halogen illuminator 2 9 3 6 3 11 halogen lamp 2 19 Harmonic Drive...

Страница 139: ...ical stage 2 3 2 22 2 24 2 25 3 5 3 7 3 8 mercury vapor short arc lamp 2 11 3 40 microscope camera 3 42 modules 1 4 1 8 multi observation equipment 2 32 3 46 3 48 N nosepiece 2 6 3 5 3 8 3 11 3 12 3 2...

Страница 140: ...8 stage carrier 2 7 2 15 2 22 2 23 stage focusing 1 18 3 9 3 13 3 21 3 22 Start up 2 1 2 3 switching on 3 18 switch on switch off 2 8 T Technical Data 1 16 technique modules 2 3 2 28 3 9 3 13 tools 4...

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