Axioskop 2 FS
plus
OPERATION
Axioskop 2 FS
MOT
Illumination and contrasting techniques
Carl Zeiss
B 40-076 e 02/01
3-21
•
Swing in 10x objective (yellow ring, see p. 1-12)
in the objective slider (3-16/
5
) and focus on the
specimen via focusing drive (3-16/
1
).
•
Close luminous-field diaphragm (3-16/
3
) until it
becomes visible (even if not in focus) in the field
of view (3-16/
A
).
•
Use drive for height adjustment (3-17/
2
) to
lower condenser until the edge of the
luminous-field diaphragm appears in focus
(3-16/
B
).
•
Use both centering screws (3-17/
3
) of the
universal condenser to center the luminous-field
diaphragm (3-16/
C
) and then open the
diaphragm until its edge just disappears from
the field of view (3-16/
D
).
•
For aperture diaphragm setting (contrast),
remove one eyepiece from the tube and look
into the tube with your naked eye. Use sliding
knob (3-16/
4
) to set the aperture diaphragm to
approx. 2/3
...
4/5 of the diameter of the
objective exit pupils (3-16/
E
). In most
applications, this aperture diaphragm setting
provides optimum contrast at almost ideal
resolution, and is therefore the best
compromise for the human eye.
•
Insert eyepiece in the tube again.
☞
Since field size and objective aperture change after every objective change, the setting of the
luminous-field diaphragm and the aperture diaphragm must be repeated to obtain optimum
results.
For < 10x objectives, the front lens of the condenser must be folded out and the aperture
diaphragm fully opened. In the case of such large fields, the luminous-field diaphragm can also
be used for better contrasting by reducing its opening until it becomes visible in the field of
view.
Fig. 3-16
Microscope settings on the
Axioskop 2 FS plus in transmitted-
light brightfield
Содержание Axioskop 2 FS plus
Страница 1: ...Operating Manual Axioskop 2 FS plus FS MOT Research Microscope...
Страница 14: ...INTRODUCTION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 0 14 B 40 076 e 02 01...
Страница 36: ...START UP Axioskop 2 FS plus Carl Zeiss List of illustrations Axioskop 2 FS MOT 2 4 B 40 076 e 02 01...
Страница 110: ...OPERATION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 3 40 B 40 076 e 02 01...
Страница 112: ...CARE MAINTENANCE TROUBLESHOOTING Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 4 2 B 40 076 e 02 01...
Страница 122: ...ANNEX Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT A 2 B 40 076 e 02 01...
