Zeiss Axioskop 2 FS plus, Руководство пользователя

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Axioskop 2 FS plus OPERATION
Axioskop 2 FS
MOT
Illumination and contrasting techniques Carl Zeiss
B 40-076 e 02/01 3-31
•
Switch on the HBO 103 or HBO 50 mercury
vapor short-arc lamp (3-23/
2 ) (see section
2.1.12, page 2-14) and allow it to heat up to its
operation temperature for approx. 15 mins.
•
Select the FL P&C reflector module in reflector
turret (3-23/
1 ) containing the required
fluorescence filter combination (depending on
the excitation) and switch it on.
•
When using the incident-light filter slider
(3-23/
4 ), unblock the light path in the incident-
light illuminator.
•
Remove one eyepiece from the tube and set
the aperture diaphragm (3-23/
3 ) by opening it
until the entire objective exit pupil is unblocked.
•
Insert eyepiece in tube again and close
luminous-field diaphragm (3-23/
5 ) until it is
visible in the field of view.
•
Use the two centering screws (3-23/ 6 ) to center
the luminous-field diaphragm in relation to the
edge of the field of view.
•
Either open the luminous-field diaphragm until
it just disappears behind the edge of the field of
view or, if there is a risk of specimen bleaching,
reduce it until it is visible in the field of view.
•
Finally, refocus on the specimen and optimize the HBO 103 collector position as described in section
2.1.14. Set the collector in such a way that homogeneous illumination is obtained with the short-
wave excitation reflector module. When modules with long-wave excitation are used, correction of
the collector position is not required.
Fig. 3-23 Components for epi-fluorescence
on the Axioskop 2 FS plus