Zeiss Axioskop 2 FS plus, Руководство пользователя

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Axioskop 2 FS plus INSTRUMENT DESCRIPTION
Axioskop 2 FS
MOT
Objectives Carl Zeiss
B 40-076 e 02/01 1-13
The exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the
numeric aperture of the objective. Therefore, certain objectives can be set for different cover slip
thickness (due to correction mount). For this, a specimen area is searched, and the position of the
correction ring where optimum focus and image contrast are obtained is determined (refocusing is
always required).
When immersion objectives are used, the air between the cover slip and the objective is replaced with a
liquid, which is immersion oil in most cases. The plastic oiler containing 20 ml of 581 N immersion oil
(n
D
= 1.515) is particularly suitable for this purpose.
To prevent oil contamination of the specimen when the nosepiece is turned, the resilient mounts of the
immersion objectives can be locked in their lifted position by turning them to the right (do not forget to
unlock them again!).
The following objectives are ideal for the Axioskop 2 FS plus and Axioskop 2 FS
MOT
microscopes:
Microscopy technique Objective Magnification /
Num. Aperture Free working
distance in mm Cover slip thickness D
in mm Cat. No.
Transmitted-light Achroplan 10x/0.30 W Ph 1 440039-0000-000
-brightfield Achroplan 40x/0.80 W 3.6 440090-9901-000
-darkfield IR-Achroplan 40x/0.80 W 3.6 440095-0000-000
-phase contrast Achroplan 63x/0.90 W 1.4 440067-0000-000
-interference contrast Achroplan 100x/1.0 W 0.97 440087-0000-000
Incident-light
-fluorescence