YHLO BIOTECH UNION, Руководство по эксплуатации

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V2.3.0 OM-UNN valid from 15
th
April 2015
30
Fig. 4-4 4PL Curve

The optical density of the calibrator OD
cal
will be calculated by the formula OD
cal
= OD
450
- OD
630

The mode of 4PL calibration can do Single-Point and two-Points. The mode of Two-Points has two
methods:
Method 1: Test only one cartridge for every calibrator on the same cycle.
Method 2: Test two cartridges for every calibrator on the same cycle and the average calibrator OD will
be used as calibrated OD.

If OD
cal0
– TOD
cal
≤ OD
cal
≤OD
cal0
+ TOD
cal
, The calibrated OD
cal
will be accepted,
Or the calibration will be cancelled and the error message will be displayed;

According the Calibrators to calibrate the 4PL curve;
All the sample concentrations will be calculated by the calibrated 4PL curve.
4.2 Units of Measurement

- index (ratio: OD of sample/OD of calibrator)

- IU/mL, International Units per milliliter

- AU/mL, Arbitrary Units per milliliter

- GPLU/mL, CardiolipinIgG antibodies per milliliter according to Harris standard

MPLU/mL, CardiolipinIgM antibodies per milliliter according to Harris standard

%, percentage

- ng/mL