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When the form of the article to be sterilized prevents air from

escaping, the efficiency of sterilization may be reduced by the

residual air. It is, therefore, recommended that the following

actions should be taken before performing sterilization:

•When using a sterilizing bag, fill it with approximately 100mL of

water and leave it open as widely as possible.

•When using a container, fill it with a small amount of water

(approximately 10 to 50mL).

•Make sure that the load does not exceed 50% of the chamber

capacity.

To perform effective sterilization

○

If the container with poor penetration is sterilized, it may be deformed or the efficiency

of sterilization may be decreased. It is, therefore, recommended that the following actions

should be taken before performing sterilization:

•Remove or loosen the lid of container before sterilization.

•Replace the cap with a highly breathable or porous one.

○

When plastic articles are sterilized, they may be deformed. It is, therefore, recommended that

sterilization should be performed referring to the table given below.

○

Table. Sterilization adaptability in the plastic articles to be sterilized

※

The above table should be referred as a guide. Please note that the characteristics of the material may

change with operating parameters, including pressure, temperature and time.

Adaptability

Polyethylene

Polypropylene

co polymer

Polypropylene

Polycarbonate

Tefron FEP

Autoclave

Not allowed

Allowed

(121℃)

Allowed

(121℃)

Caution needed

Allowed

(121℃)

Water

absorption ratio (%)

0.02 or less

0.03 or less

Approx. 0.3

0.01 or less

Transparency

Opaque

Translucence

Transparence

Translucence

Place articles to be sterilized in the stainless steel basket provided or in an optional stainless

steel bucket.

※ Stainless steel buckets and baskets are available as optional containers.

※

Make sure that the articles to be sterilized will not touch the chamber lid gasket or the temperature

sensor when they are placed in the chamber. Otherwise, it may cause damage.

Place articles to be sterilized carefully in the chamber.

※

When loading the container with articles to be sterilized, make sure that the articles to be sterilized

never exceeds the load line or the upper surface of the container. Otherwise, the articles to be

sterilized may be damaged or broken when the chamber lid is closed.

Close the chamber lid.

Check the lid open indicator turns off.

To place articles to be sterilized in the chamber

①

②

③

④

Содержание FLS-1000

Страница 1: ...sed for the handling of microorganisms such as bacteria and viruses which can exert adverse health effects on human beings or other living things therefore must be used by personnel with enough knowledge and experience Be sure to keep this Operator s Manual handy for future reference OPERATOR S MANUAL AUTOCLAVE FOR RESEARCH USE ...

Страница 2: ...amber 6 USEFUL FUNCTIONS 6 1 Liquid Sterilization Function 6 2 Memory Function 6 3 Check Function 6 4 Timer Function 6 5 Power Failure Alert Function 6 6 Annual Inspection Reminder Function 6 7 Setting the Time Unit for Display 6 8 Selecting the Exhaust Setting for the Liquid Sterilization Course 6 9 Setting the Temperature to Release the Lid Interlock 6 10 Sterilizing Water Change Alert Function ...

Страница 3: ...ation Safety indications are classified into Warnings and Cautions depending on the level of potential injury to personnel or damage to the equipment Indicates a potentially hazardous situation which if not avoided could result in death or serious injury to laboratory personnel 1 PRECAUTION FOR USE WARNING CAUTION Indicates a potentially hazardous situation which if not avoided could result in min...

Страница 4: ... do so may cause short circuit fire or malfunction Do not remove the outer panel of the FLS 1000 Contact with the inner parts of FLS 1000 may cause electric shock burns fire or malfunction Do not touch the power plug with a wet hand Failure to do so may cause electric shock Do not use any damaged power cord plug or loose outlet Failure to do so may cause accidental fire short circuit or malfunctio...

Страница 5: ...r lid Steam coming out of the chamber is very hot and can cause burns or injury Do not use the FLS 1000 to sterilize sealed articles Sealed articles may explode or burst when they are removed from the chamber thereby causing burns injury or serious accident The chamber pressure may become abnormally high thereby causing damage to parts Broken parts may scatter and cause burns injury or serious acc...

Страница 6: ...empty heating prevention function resulting in fire Be sure to unplug the FLS 1000 before performing maintenance procedures Failure to do so may cause electric shock For models with no power plug provided the power circuit breaker to which the power plug is connected should be turned off Do not operate the FLS 1000 if any corrosion damage deformation or loose connections are found on its chamber c...

Страница 7: ...ter from the chamber Water in the chamber or the exhaust tank may be very hot after the operation causing burns Be careful of steam coming out of the steam exhaust vent and its surrounding area during the operation Hot steam may cause burns Allow chamber to cool down sufficiently before performing maintenance work Otherwise high temperature inside the chamber may cause burns Make sure that there i...

Страница 8: ...mperature is reached the timer starts to maintain the set temperature When the set sterilizing time has elapsed the heater stops The sterilizing action is determined by the three elements temperature humidity and time that are applied to articles to be sterilized during this process of the sterilization cycle When the chamber temperature drops the valve opens to allow the pressure inside the chamb...

Страница 9: ...ー 8 ー MEMO ...

Страница 10: ...ー 9 ー 2 2 Parts Identifications FLS 1000 Main Unit ...

Страница 11: ...ttings Safety valve access port This opening closing cover provides access to the safety valve for inspection Power supply switch It is used to turn on or off the power to FLS 1000 Leakage breaker It is a safety device and kept on during normal operation Accessory case mounting hole It is provided to mount the accessory case for storing the operator s manual Main nameplate Product Label Power cord...

Страница 12: ...the closed position Lid hook boot Lid interlock switch It activates the lid interlock Lid hook catch Chamber lid Chamber lid gasket It maintains air tightness of the pressure vessel Lid fixing nut Safety valve hole Chamber Hinge Hinge shaft Retaining ring ...

Страница 13: ...vel of the exhaust tank Exhaust tank Air and steam exhausted from the chamber is collected into this exhaust tank Exhaust tank drain hose cap Drain hose for exhaust tank It allows water to drain from the exhaust tank Exhaust tank cap Drain cock Drain hose for chamber LOWER UPPER ...

Страница 14: ...ber through this hole The hole leads to the exhaust tank the trap bottle and the steam exhaust vent Water level sensor It detects the level of sterilizing water in the bottom of the chamber Drain hole Sterilizing water in the chamber is drained through this hole The hole leads to the drain port Chamber bottom plate It is provided to prevent articles to be sterilized from contacting with a heater a...

Страница 15: ...ー 14 ー Piping Diagram PS P Pressure gauge Pressure sensor M Ball valve Exhaust tank Air release valve Trap bottle Safety valve ...

Страница 16: ...ot be started while it is on Drain indicator It turns on when the exhaust tank is full and must be drained The FLS 1000 cannot be started while it is on Drain access cover open indicator It turns on when the drain access cover at the front of the main body is opened The FLS 1000 cannot be started while it is on Sterilizing water change indicator It turns on when the water in the sterilization cham...

Страница 17: ...ing keys Press these up down arrow keys to increase or decrease the set time for the sterilization cycle STERILIZE LIQUID key Press this key to select the liquid sterilization course while the FLS 1000 is in standby mode STERILIZE key Press this key to select the sterilization course while the FLS 1000 is in standby mode J K L M MEMORY key Press and hold down this key in standby mode to save the c...

Страница 18: ...he process of temperature and pressure falling is currently being performed Temperature falling process indicator It turns on to indicate that the temperature falling process is currently being performed READY indicator It turns on to indicate that the FLS 1000 is ready for operation POWER FAILURE indicator It turns on to indicate that a power failure occurs during an operation Please refer to 6 5...

Страница 19: ...alling the FLS 1000 in a place where there is a lot of dust or moisture Failure to do so may cause short circuit of electric components or fire Avoid installing the FLS 1000 in or near a sink or tap where there is a risk of it being splashed with water Failure to do so may cause short circuit or electric shock Install the FLS 1000 on a sturdy and level surface such as concrete floors that can supp...

Страница 20: ...oom that is well ventilated and free of direct sunlight Place the FLS 1000 onto a solid flat surface such as a concrete floor Weight of the FLS 1000 108kg Avoid installing the FLS 1000 in a slippery place or a place which may be exposed to vibration Considering the steam coming from steam exhaust vent and surrounding area install FLS 1000 providing a clearance of at least 25cm at the rear and on e...

Страница 21: ...additional 90 1 4 turn with a spanner 19mm width to fix it on the floor Inspect for any possible shake or wobble by pushing the unit from all four corners The control panel can be moved either in the right or left direction so that it faces the front allowing users easy access to the control panel Hold the control panel with both hands and move it slowly Fix the clear plastic accessory case for st...

Страница 22: ...y cause explosion electric shock or malfunction To protect against electric shock adequate grounding of FLS 1000 must be provided When it cannot be provided arrange for a qualified electrician to carry out the necessary grounding work The FLS 1000 employs a plug with a two pole grounding electrode Plug the FLS 1000 into a two pole socket outlet of the voltage specified as correct for FLS 1000 For ...

Страница 23: ...titude level if it is moved to another location Table Altitude level code and the corresponding altitude level and maximum setting sterilization and lid interlock releasing temperatures Altitude level code Altitude level Maximum setting sterilization temp Maximum lid interlock releasing temp 0 Below 800m 123 100 1 Above 800m below 1500m 122 97 2 Above 1500m below 2000m 121 95 Press and hold down t...

Страница 24: ...rticles to be sterilized P 31 6 Setting operating parameters P 33 7 Starting operation P 35 8 Ending operation P 37 9 Removing articles to be sterilized P 38 10 Draining the sterilizing water from the chamber P 39 11 Turning off the power switch P 24 For repetitive operation restart the operation with 2 Checking the pressure gauge after performing 9 Removing articles to be sterilized on the above ...

Страница 25: ...pears in the time display on the control panel To turn on the power Press the power switch to the OFF position Check that the IN PROGRESS indicator on the control panel turns off or the COMPLETED indicator in the work monitor display flashes To turn off the power Operating parameters of the previous operation will be displayed in approximately 10 seconds STOP MEMORY STERILIZE LIQUID START FUNCTION...

Страница 26: ... death or serious injury CAUTION Be extremely careful of steam coming out of the chamber when opening the chamber lid Steam coming out of the chamber is very hot and can cause burns or injury Do not place hands on the top plate Otherwise fingers or hands may get caught in the chamber lid resulting in injury Avoid leaving any foreign materials between the chamber lid gasket and the chamber Failure ...

Страница 27: ...hamber lid handle Place hands on the chamber lid handle and push it down until the lid open indicator turns off To close the chamber lid When the power switch is turned on with the lid being closed the lid open indicator turns on Open and then close the lid to activate the lid open close detection system The chamber lid may not be closed easily when the chamber temperature is still high immediatel...

Страница 28: ...top operation Otherwise abnormality inside the chamber if any cannot be found thereby causing serious accident If pressure gauge malfunction is suspected please contact your dealer or our local representative Open the chamber lid Check that the pressure gauge reads 0MPa To check the pressure gauge ...

Страница 29: ...r level in the exhaust tank is between the maximum water level and the minimum water level Visually check the water level in the exhaust tank by looking through a slit on the drain access cover Pour water into the exhaust tank if the water level is lower than the minimum level Drain the exhaust tank if the water level exceeds the maximum level For details on the illumination function please refer ...

Страница 30: ...all the cap onto the end of the hose Insert the drain hose into the space on the left side of the exhaust tank Close the drain access cover Check the drain access cover open indicator turns off To drain water from the exhaust tank Check that the water inside the exhaust tank is cooled down Open the drain access cover Remove the exhaust tank cap Pour water in the exhaust tank through the feed water...

Страница 31: ... sterilizing water The FLS 1000 generates steam by heating up the sterilizing water in the chamber which has a heating element in its bottom Check the quality and level of the sterilizing water inside the chamber to perform adequate sterilization Use tap water as sterilizing water When purified water or cold water is used the water level sensor may not correctly detect the level of the sterilizing...

Страница 32: ...d glassware may explode or burst when it is removed from the chamber thereby causing burns injury or serious accident Make sure that holes inside the chamber are not blocked by articles to be sterilized If the vent hole is blocked by such items as sterilization bags the chamber pressure cannot be controlled thereby causing serious accident such as explosion of the chamber Make sure that articles t...

Страница 33: ... to be sterilized The above table should be referred as a guide Please note that the characteristics of the material may change with operating parameters including pressure temperature and time Adaptability Polyethylene Polypropylene co polymer Polypropylene Polycarbonate Tefron FEP Autoclave Not allowed Allowed 121 Allowed 121 Caution needed Allowed 121 Water absorption ratio 0 02 or less 0 02 or...

Страница 34: ...alling processes To set the sterilization operating parameters 5 7 Setting Operating Parameters To change the time display format refer to 6 7 Setting the Time Display Format The upper limit of the temperature setting range varies depending on the altitude level selected at the time of installation of the autoclave Altitude level code Altitude level Sterilization process Temp setting range Time se...

Страница 35: ... minutes 12 minutes 1L of water in a container for 20 minutes 17 minutes 2L of water in a container for 20 minutes 28 minutes 5L of water in a container for 20 minutes 38 minutes 10L of water in a container for 20 minutes 67 minutes To perform effective sterilization The FLS 1000 controls the temperature using the temperature detected by the temperature sensor in the chamber as an index When more ...

Страница 36: ...ealer or our local representative CAUTION Be careful of steam coming out of the steam exhaust vent and its surrounding area during the operation Hot steam may cause burns Keep away from the FLS 1000 while it is in operation Failure to do so may cause burns Please keep at least 50 cm away from all four corners of the autoclave while it is in operation Do not attempt to touch the outer panel of the ...

Страница 37: ...T FUNCTION CHECK TIMER EXT SENSOR COOLING FAN EXHAUSTSPEED FAST TIME TEMPERATURE TIMER READY STERILIZE LID LID WATER DRAIN COVER CHANGE WATER C SLOW STERILIZE INPROGRESS STOP MEMORY STERILIZE LIQUID START FUNCTION CHECK TIMER EXT SENSOR COOLING FAN EXHAUSTSPEED FAST TIME TEMPERATURE TIMER STERILIZE LID LID WATER DRAIN COVER CHANGE WATER C SLOW STERILIZE INPROGRESS While the lid open low sterilizin...

Страница 38: ...ow on Off On Lid interlock release temperature reached Operation completed Yellow on Flashes normal Off abnormal Off 6 beeps 60 reached Off On normal Off abnormal Off 10 beeps Table Operation of the indicator and buzzer in each process of the sterilization cycle 5 9 Ending Operation When the chamber temperature reaches the lid interlock release temperature the sterilization cycle is completed and ...

Страница 39: ...ause burns or unexpected accident Do not leave any contamination inside the chamber or in the sterilizing water Failure to do so may cause corrosion or damage on the chamber It may also cause malfunction of the water level sensor which may hamper the empty heating prevention function resulting in fire Check that the gauge for pressure in the chamber reads 0MPa Check that the lid interlocked indica...

Страница 40: ...rotating it slowly and drain the sterilizing water from the drain port If the water drains very slow or does not drain the port may be clogged Clean the drain port with tools such as a wire to remove the clog After completely draining water from the chamber close the drain cock Place the drain hose back to its original position Close the drain access cover Close the chamber lid Open Close WARNING ...

Страница 41: ... course is selected while all process indicators on the work monitor light up green Press the temperature setting up and down arrow keys to set the temperature for the sterilization process Press the time setting up and down arrow keys to set the sterilization time for the sterilization process Parameters for the exhaust speed and cooling fan cannot be preset These parameters can only be set durin...

Страница 42: ... Function The check function allows operators to view the set temperature and time while the display shows the actual value of temperature The display view can be toggled between set and actual values by using this function Press and hold down the CHECK key while the IN PROGRESS indicator is on Set values for the temperature and time are displayed while the key is pressed To check set values for t...

Страница 43: ...tion The timer function allows the operator to set the waiting time for the operation to start To set the timer follow the procedures given below after setting the desired operating parameters STOP MEMORY STERILIZE LIQUID START FUNCTION CHECK TIMER EXT SENSOR COOLING FAN EXHAUSTSPEED FAST TIME TEMPERATURE TIMER READY STERILIZE LID LID WATER DRAIN COVER CHANGE WATER C SLOW STERILIZE INPROGRESS STOP...

Страница 44: ... this function the COMPLETED indicator will not turn on after the operation is completed When the error code A09 appears in the display STOP MEMORY STERILIZE LIQUID START FUNCTION CHECK TIMER EXT SENSOR COOLING FAN EXHAUSTSPEED FAST TIME TEMPERATURE TIMER POWER FAILURE STERILIZE LID LID WATER DRAIN COVER CHANGE WATER C SLOW STERILIZE INPROGRESS STOP MEMORY STERILIZE LIQUID START FUNCTION CHECK TIM...

Страница 45: ...ow keys to show F19 in the time display The temperature display shows the month day of the current date Check that the date displayed is correct Press and hold down the START key The buzzer will sound in approximately 4 seconds to indicate that the setting is finalized and the display will return to normal display STOP MEMORY STERILIZE LIQUID START FUNCTION CHECK TIMER EXT SENSOR COOLING FAN EXHAU...

Страница 46: ...n the START key The buzzer will sound in approximately 4 seconds to indicate that the setting is finalized and the display will return to normal display Code Time unit for display Display range Setting range 0 Hour minute 0 00 9 59 0 01 9 59 1 Hour 0 99 hours 1 99 hours 2 Minute 0 999 minutes 1 999 minutes 3 Hour minute hour 0 01 9 59 10 99 hours 0 01 9 59 10 99 hours The factory default values fo...

Страница 47: ...he exhaust valve opening can be selected for the exhaust speed in the liquid sterilization course Press and hold down the START key The buzzer will sound in approximately 4 seconds to indicate that the setting is finalized and the display will return to normal display Code Exhaust setting Description 0 Speed level setting Six levels 1 Variable setting Variable STOP MEMORY STERILIZE LIQUID START FU...

Страница 48: ...n the altitude level Refer to 3 3 Setting the Altitude Level The lid interlock release temperature can also be monitored by the external temperature sensor for articles to be sterilized Refer to 7 1 Temperature Sensor for Articles to be Sterilized 6 9 Setting the Temperature to Release the Lid Interlock The FLS 1000 is equipped with the lid interlock which activates and prevents the lid from openi...

Страница 49: ... activates the water change alert function can be changed depending on the level of contamination in water To set the change alert STOP MEMORY STERILIZE LIQUID START FUNCTION CHECK TIMER EXT SENSOR COOLING FAN EXHAUSTSPEED FAST TIME TEMPERATURE TIMER READY STERILIZE LID LID WATER DRAIN COVER CHANGE WATER C SLOW STERILIZE INPROGRESS Press the FUNCTION key while the IN PROGRESS indicator is off The ...

Страница 50: ...ow F13 in the time display The temperature display shows the code of the illumination function Press the START key The temperature display starts to flash Press the temperature setting up and down arrow keys to select the desired code of the illumination function Press and hold down the START key to finalize the setting The buzzer will sound in approximately 4 seconds and the display will return t...

Страница 51: ...ー 50 ー MEMO ...

Страница 52: ...erturn prevention strap or wire The external temperature sensor for articles to be sterilized Sterilization process is monitored using the temperature sensor for articles to be sterilized by installing it directly into the article in the chamber so that the autoclave begins the sterilization time countdown only when the temperature for articles to be sterilized has reached a set sterilization temp...

Страница 53: ...e the chamber may cause burns Wipe the unit with a soft cloth moistened with a mild detergent Wipe away any detergent residue using a cloth dipped in clear water and squeezed almost dry To clean the main body If the main body exterior or the chamber interior of the FLS 1000 becomes stained or dirty clean or disinfect it according to the following procedures 8 1 Cleaning and Disinfecting the Main B...

Страница 54: ...ber interior and water level sensor Remove the chamber bottom plate carefully Wipe the chamber interior and the water level sensor using a mild detergent and a cloth or other suitable materials and wash away any detergent residue using running water and drain the water Be careful not to distort sensors in the chamber while cleaning To clean each part To ensure safe operation of the FLS 1000 perfor...

Страница 55: ...ng rings lid hooks and lid catches for corrosion cracks or other damage Check safety valve for any signs of corrosion cracks or other damage The safety valve can be visually inspected through the inspection port by removing its cover with a screwdriver After inspection reinstall the cover 3 Power plug Unplug the autoclave Wipe off any dust or dirt on the surface of the power plug using a dry cloth...

Страница 56: ... on Fill the chamber with water Drain indicator is on Drain the exhaust tank Drain access cover open indicator is on Close the drain access cover Sterilizing water change indicator is on Change water in the chamber Temperature rises slowly Extremely large amount of water is in the chamber Check and adjust a level of water in the chamber FLS 1000 is loaded with a large amount of articles to be ster...

Страница 57: ...ower than the set value Unplug the power plug of the FLS 1000 from the outlet and contact your dealer or our local representative E09 Chamber lid opens during the operation Lid open is detected by the sensor Close the chamber lid securely If this happens during the operation unplug the power plug of the FLS 1000 from the outlet and contact your dealer or our local representative E10 Interlocking s...

Страница 58: ...dicted by the chamber temperature by equal to or less than 30kPa Turn off the power switch and wait until the chamber pressure becomes 0MPa to open the lid Take out and place the load again so that the hole in the chamber is not blocked Open the sterilization bag if it is closed E25 Control system malfunction Memory abnormality Unplug the power plug of the FLS 1000 from the outlet and contact your...

Страница 59: ... process has started or before it has completed Refer to 6 5 Power Failure Alert Function A10 The memory approaches the end of its lifetime E26 An alarm notification appears when 80 90 95 98 99 and 99 5 of the lifetime is reached Press the check key once to clear the error and enable the system A20 The start key is pressed while printing Press the check key once A21 Printer trouble Printer error i...

Страница 60: ...dioactive substances In cases where FLS 1000 or any parts of FLS 1000 has any residue of blood or other chemicals and has been identified as hazardous 1 2 For Assistance or Service Contact TOMY DIGITAL BIOLOGY CO LTD 3 14 17 Tagara Nerima ku Tokyo 179 0073 Japan e mail info digital biology co jp URL http www digital biology co jp phone 81 3 5971 8160 fax 81 3 3970 6036 TOMY SEIKO CO LTD If any sys...

Страница 61: ... been exposed to infectious and hazardous or radioactive substances In cases where FLS 1000 or any parts of FLS 1000 has any residue of blood or other chemicals and has been identified as hazardous 1 2 10 DISPOSAL AND TRANSFER To ensure smooth operations for product assurance we implement and maintain adequate record keeping systems Please contact your dealer or our local representative when you d...

Страница 62: ...sure sensor malfunction detection Exhaust valve malfunction detection Low sterilizing water level detection Chamber lid open close detection Drain access cover open detection Exhaust tank full level detection Leakage breaker Rated breaking current 15A Rated sensitivity current 10mA Protection against electric shock Class I Time Display form Digital Display range Sterilize 0 00 9 59 0 99hours 0 999...

Страница 63: ... Atmospheric pressure Relative humidity Altitude For indoor use only Overvoltage category Pollution degree Maximum gradient from 10 to 35 from 750 to 1060hPa 75 or less not exceeding 2000m II 2 2 Accessories Digital warranty information Inspection sheet Specifications of small sized pressure vessel Operator s manual English Operator s manual English OPTIONAL FUNCTIONS Chamber bottom plate Stainles...

Страница 64: ...21023 00 Issued in August 2018 TOMY KOGYO CO LTD TOMY SEIKO CO LTD TOMY DIGITAL BIOLOGY CO LTD 3 14 17 Tagara Nerima ku Tokyo 179 0073 Japan e mail info digital biology co jp URL http www digital biology co jp Phone 81 3 5971 8160 fax 81 3 3970 6036 ...

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