Thermo Scientific
imPULSE S.U.M. with Touchscreen Console User’s Guide
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Chapter 6
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Operating information
Figure 6.7. Harvest Control screen.
5. You can select
Pause
at the bottom of the screen at any time
to temporarily stop the harvesting process. The Harvest Paused
screen will appear, which allows you to either resume or cancel the
harvest process.
6.7.2 Harvesting manually
If you are not using pumps per section 3.1, you must manually drain the
imPULSE S.U.M. Use the following instructions to harvest manually.
Harvesting manually with a recirculation loop
If you are using a recirculation loop, the drain line is located on the
addition line “Y.” Open the clamp on the 30.5 cm (12 in.) section of
tubing on the recirculation loop “Y” and connect to the intended
transfer line. Use the peristaltic pump already installed on the
recirculation line to transfer the contents of the imPULSE S.U.M. Be
sure to stop the mixing motor before you reach the minimum working
volume.
Harvesting manually without a recirculation loop
If you are not using a recirculation loop, follow the steps below to
manually harvest liquid from the imPULSE S.U.M. BPC.
1. Connect the bottom drain tubing set to the intended transfer line.
Note:
For sterile connections, this must be done aseptically.
2. Open the clamp that is positioned at the bottom drain port.
3. Begin draining the BPC using a peristaltic pump. Be sure to stop
the mixing motor before the liquid in the BPC reaches the minimum
working volume.
