Thermo 335904, Руководство оператора, Страница: 82 / 94

Appendix C - Calculations
Thermo Electron Corporation C-2
Test Name Calculation(s) Default Displayed
Parameters Results
dsDNA Conc. = (F x A
260
)D
f
260nm µ g/mL
Factor
dsDNA
= 50
Conc. = (F x A
260
)D
f
260nm
µ
g/mL
ssDNA, RNA Factor
ssDNA
or
RNA
= 40
Oligos Conc. = (F x A
260
)D
f
260nm
µ
g/mL
(entered factor) Factor
oligos
= 33
Oligos (calc factor) Conc. = (F x A
260
)D
f
260nm
µ
g/mL
F = factor calculated by Oligo Calculator
Bradford Second order 595nm µ g/mL
– standard Std. Conc.
of 0, 200, 400,
600, 800,1000
Bradford – micro Second order 595nm
µ
g/mL
Std. Conc.
of 0, 20, 40, 60,
80, 100
Lowry – standard Second order 550nm
µ
g/mL
Std. Conc.
of 0, 100, 200,
500, 1000, 2000
Lowry – micro Second order 750nm
µ
g/mL
Std. Conc.
of 0, 20, 50,
100, 200, 500
Second order 562nm mg/mL
(BCA) – standard Std. Conc.
of 0, 0.2, 0.4,
0.6, 0.8, 1.0
Second order 562nm
µ
g/mL
(BCA) – micro Std. Conc. of 0,
0.5, 1, 2, 5, 10
Biuret First order through zero 540nm mg/mL
Std. Conc. of 0,
2, 4, 6, 8, 10
Direct UV (280) Conc. = (F x A
280
)D
f
280nm mg/mL
Factor
280
= 1
Direct UV (205) Conc. = (F x A
205
)D
f
205nm mg/mL
Factor
205
= 31
Warburg-Christian Dilution Factor (D
f
) = diluent vol. + sample vol. A
1
= 280nm mg/mL
sample volume A
2
= 260nm
f
1
= 1.55
Protein Concentration = [(A
1
)f
1
– (A
2
)f
2
] D
f
f
2
= 0.76
Cell growth None 600nm Abs