Thermo 335904, Руководство оператора, Страница: 81 / 94

Appendix C - Calculations
C-1 BioMate 3 Operator’s Manual
Calculations
Table 1 Calculations for “Biotest” Software
Test Name Calculation(s) Default Displayed
Parameters Results
DNA/Protein Dilution Factor (D
f
) = diluent vol + sample volume A
1
= 260nm
µ
g/mL
(260, 280) sample volume A
2
= 280nm
A
ref
= 320nm
DNA concentration = [(A
1
– A
ref
)f
1
– (A
2
– A
ref
)f
2
]D
f
(optional)
f
1
= 62.9
Protein concentration = [(A
2
– A
ref
)f
3
– (A
1
– A
ref
)f
4
] D
f
f
2
= 36.0
f
3
= 1552
Ratio = A
1
- A
ref
f
4
= 757.3
A
2
- A
ref
dil.vol. = 0
smp.vol = 1
DNA (260, 230) Dilution Factor (D
f
) = diluent vol + sample volume A
1
= 260nm
µ
g/mL
sample volume A
2
= 230nm
A
ref
= 320nm
DNA concentration = [(A
1
– A
ref
)f
1
– (A
2
– A
ref
)f
2
] D
f
(optional)
f
1
= 49.1
Protein concentration = [(A
2
– A
ref
)f
3
– (A
1
– A
ref
)f
4
] D
f
f
2
= 3.48
f
3
= 183
Ratio = A
1
- A
ref
f
4
= 75.8
A
2
- A
ref
dil vol. = 0
smp.vol = 1
DNA (260, 280) Dilution Factor (D
f
) = diluent vol + sample volume Start wavelength
µ
g/mL
with Scan sample volume = 225nm
Stop wavelength
DNA concentration = [(A
1
– A
ref
)f
1
– (A
2
– A
ref
)f
2
] D
f
= 325nm
A
1
= 260nm
Protein concentration = [(A
2
– A
ref
)f
3
– (A
1
– A
ref
)f
4
] D
f
A
2
= 280nm
A
ref
= 320nm
Ratio = A
1
- A
ref
(optional)
A
2
- A
ref
f
1
= 62.9
f
2
= 36.0
f
3
= 1552
f
4
= 757.3
dil.vol. = 0
smp.vo. = 0
DNA (260, 230) Dilution Factor (D
f
) = diluent vol + sample vol. A
1
= 260nm µ g/mL
with Scan sample volume A
2
= 230nm
A
ref
= 320nm
DNA concentration = [(A
1
– A
ref
)f
1
– (A
2
– A
ref
)f
2
] ] D
f
(optional)
f
1
= 49.1
Protein concentration = [(A
2
– A
ref
)f
3
– (A
1
– A
ref
)f
4
] D
f
f
2
= 3.48
f
3
= 183
Ratio = A
1
- A
ref
f
4
= 75.8
A
2
- A
ref
dil.vol. = 0
smp.vol. = 1