Thermo 335904, Руководство оператора, Страница: 30 / 94

Note: If Cell Correction is ON, you must run the
Setup Correction program before you can
access the Run Test or Measure
Samples keys.
Setting up test parameters
1. With the Direct UV (280) setup screen
displayed, use the arrow keys to highlight the
name of the parameter you want to set.
2. When the parameters are set, you can press
Save Test to save the test or to measure the
blank and unknowns.
Measuring the sample
Measuring unknowns automatically (using Auto 6
or Auto 3)
1. With the Direct UV (280) screen displayed,
place the blank and unknowns in the correct
cell positions.
2. Press ENTER to start the measurements. The
instrument automatically measures the blank
first, then measures the unknowns and
displays the absorbance and concentration of
each unknown.
Measuring unknowns manually (using Manual 6 or
Single Cell Positioner)
1. With the Direct UV (280) screen displayed,
press Run Test .
2. Place the blank and unknown in the correct cell
positions. If your instrument is equipped with a
6-Position Cell Holder, you can place up to five
samples in the cell holder.
3. Press Measure Blank to measure the blank. If
your instrument is equipped with a 6-Position
Cell Holder, it automatically moves to the B
position to measure the blank. When it
completes the measurement, it returns to its
previous cell position.
4. Press Measure Sample to start the
measurement. If your instrument is equipped
with a 6-Position Cell Holder, press the cell
position buttons on the keypad to re-position
the cell holder and measure the rest of the
unknowns manually.
Warburg-Christian
The Warburg-Christian analysis uses an
absorbance difference measurement at 280 and
260nm to determine the protein concentration of an
unknown. Refer to Appendix B for a description of
the parameters and Appendix C for the default
values.
To get started, move the arrow keys on the
BioMate tests screen to highlight Protein Tests
and press ENTER . Then the move the arrow keys
to highlight Warburg-Christian and press ENTER .
The Warburg-Christian parameter screen
appears.
Note: If Cell Correction is ON , you must run the
Setup Correction program before you can
access the Run Test or Measure
Samples keys.
Setting up test parameters
1. With the Warburg-Christian setup screen
displayed, use the arrow keys to highlight the
name of the parameter you want to set.
2. When the parameters are set, you can press
Save Test to save the test or Run Test to
measure the blank and unknowns.
Using “Biotests” Software
Thermo Electron Corporation 2-10