Thermo Scientific thermoscientific Vanquish UHPLC System, Руководство по эксплуатации

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8 • Troubleshooting
UHPLC System
Operating Manual Page 119
8.2.4 Flow
No flow
Possible Cause Remedial Action
Leak in system Find and eliminate the leak.
Inlet and/or outlet
check valve
contaminated or
defective Clean the inlet check valve or outlet check valve, and replace the check valve if
necessary (refer to
Pump Operating Manual ).
Air trapped in pump
heads Purge the pump (refer to Pump Operating Manual ) and check in the Chromeleon
software whether the degasser has reached the operating vacuum
(Degasser Vacuum = OK).
Air trapped in
autosampler flow path Perform a wash cycle (refer to Split Sampler Operating Manual ).
Column temperature
too high – eluent
evaporating Select a lower column temperature (refer to Column Compartment Operating
Manual ).
Flow fluctuations
Possible Cause Remedial Action
Pump inlet path
clogged Check the solvent inlet lines, solvent line filters, and solvent selectors/proportioning
valve, for example, for signs of clogging.
Air trapped in pump
inlet path • Purge the pump.
• Inspect the degasser for indications for leakage. Check in the Chromeleon
software whether the degasser has reached the operating vacuum
(Degasser Vacuum = OK). Degassing may not work properly if the flow rate is too
high.
• Inspect the capillary and solvent line connections for leakage; tighten loose fitting
connections.
Inlet and/or outlet
check valve
contaminated or
defective Clean the inlet check valve or outlet check valve, and replace the check valve if
necessary (refer to
Pump Operating Manual ).
Piston seals not sealing
tightly Replace the piston seals (refer to Pump Operating Manual ).