Thermo Scientific thermoscientific Vanquish UHPLC System, Руководство по эксплуатации

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8 • Troubleshooting
UHPLC System
Operating Manual Page 117
Possible Cause Remedial Action
Electrical interferences
from other instruments Avoid operation of equipment generating strong electric or magnetic fields in close
proximity. Do not connect equipment that may cause power disturbances to the
same power network used to supply the system.
Column temperature
significantly above
boiling point of mobile
phase • Install a restrictor at the flow cell outlet, observing the pressure specification of
the respective flow cell and the general guidelines for the flow cell.
Note : Excessive backpressure will destroy flow cells!
• Install a post-column cooler (refer to Column Compartment Operating Manual ).
8.2.3 Peak Area Precision
Contamination or carry-over
Possible Cause Remedial Action
Contamination in the
system Flush the system using an appropriate solvent.
Autosampler carry-over The needle may be contaminated. Wash the needle in the wash port. If carry-over
persists, replace the needle seat (refer to
Split Sampler Operating Manual ).
Environmental conditions
Possible Cause Remedial Action
Unstable
environmental
conditions Make sure that the temperature and air humidity are constant. Use column
thermostatting. Avoid draft. Use insulation on the capillary from the column
compartment to the detector.
Capillary problems
Possible Cause Remedial Action
Capillary connections
not installed properly
or not tight, dead
volumes in capillary
connections Properly install the capillary connections. Tighten the capillary connections. Replace
capillary.