Thermo Scientific ISQ, Руководство пользователя

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2
Changing the Column
Connecting the Column to the Transfer Line
Thermo Scientific ISQ User Guide 13
Connecting the Column to the Transfer Line
When connecting the column to the transfer line, you may use either the regular transfer line
nut or the spring loaded transfer line nut with the graphite Vespel™ ferrule.

To connect the column using the regular transfer line nut
1. Lower the oven temperature and allow it to cool.
2. Confirm that the MS is vented and remove the current transfer line nut and ferrule.
3. Unwind about one turn of the column from the column outlet end.
4. Wipe approximately 300 mm (12 in.) of the column with a tissue soaked in methanol.
5. Choose an appropriate ferrule for the outer diameter of your column.
6. Insert the column through the transfer line nut and ferrule, entering through the tapered
end of the ferrule. Wipe the column again with a tissue soaked in methanol.
Figure 5. Transfer Line Nut and SilTite Ferrule Orientation
7. Insert the column into the column measuring tool (see Figure 6 ), which is in the ISQ
Toolkit, so that it is even with the lines at the end of the column. Figure 7 indicates
proper positioning of the column in the tool for accurate measuring.
Note Wear clean, lint- and powder-free gloves when you handle the column and
transfer line ferrule.
Note If the maximum oven temperature in your method is
≥
290 °C (554 °F),
Thermo Fisher Scientific recommends using a spring loaded transfer line nut with a
graphite Vespel ferrule or a SilTite™ nut and ferrule. By cycling the oven at and above
this temperature, expansion and contraction of the graphite Vespel material can cause
leaks in the transfer line.
Transfer Line Nut
SilTite Ferrule
Flat on the
Transferline Nut