Thermo Scientific Ion 520, Руководство пользователя

Страница: 9 / 78

Ion S5 ™ Controls Kit Plus
The Ion S5
™
Controls Kit Plus (Cat. No. A30729 ) is ordered separately. See the Ion S5
™
Controls Kit Plus
Product Information Sheet (Pub. No. MAN0016206) for details.
Contents Amount Storage
Human CEPH Genomic DNA Control (red cap) 30 µL −30°C to −10°C
Human CEPH Control 200 Library (yellow cap) 12 µL
E. coli DH10B Control 400 Library (orange cap) 16 µL
E. coli DH10B Control 600 Library
The E. coli DH10B Control 600 Library includes sufficient material for 4 templating runs on the
Ion Chef
™
System. The E. coli DH10B Control 600 Library (Cat. No. A32470) is ordered separately.
Contents Amount Storage
E. coli DH10B Control 600 Library (lilac cap) 20 µL –30°C to –10°C
Required materials not supplied
Unless otherwise indicated, all materials are available through thermofisher.com . "MLS" indicates that
the material is available from fisherscientific.com or another major laboratory supplier.
Item Source
Ion Chef
™
System 4484177
Microcentrifuge
[1]
MLS
2
‑
, 20
‑
, 200
‑
, and 1,000
‑
µL pipettes and appropriate filtered tips MLS
Microcentrifuge tubes, 1.5-mL or 1.7-mL MLS
Vortex mixer with a rubber platform MLS
Gloves, powder-free nitrile MLS
Ice buckets and ice —
Nuclease-free water, molecular biology grade MLS
Isopropyl alcohol, 70% solution MLS
Wipes, disposable lint-free MLS
(Optional) Uninterruptible Power Supply (UPS)
[2]
MLS
[1]
Must fit standard 0.2- and 1.5
‑
mL microcentrifuge tubes and generate 21,000 × g . To convert the RPMs of your centrifuge to RCF in
units of gravity, see tools.thermofisher.com/content/sfs/brochures/TR0040-Centrifuge-speed.pdf .
[2]
For laboratories that experience frequent power outages or line voltage fluctuations, we recommend that you use an uninterruptible
power supply that is compatible with 2500 W output or higher.
Chapter 1
Product information
Required materials not supplied
1
Ion 520 ™ & Ion 530 ™ ExT Kit − Chef User Guide
9