Thermo Scientific Invitrogen Countess II FL, Руководство пользователя

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4. Press Adjust to open the Adjust count parameters screen, which contains the
controls for adjusting the count parameters in the selected channel.
Note:
For a description of the count parameters and count parameter controls
(i.e., parameter sliders), see “Count parameters“ on page 15.
5. Optional: Press the Graph button to view the distribution of cells based on
size or fluorescence intensity (“Graph count results“ on page 36).
6. Select the brightfield channel (white circle) to adjust the thresholds for size,
brightness, and circularity using the size, brightness, and circularity sliders.
7. Select the desired fluorescence channel (colored circles) to adjust the threshold
for fluorescence intensity using the fluorescence intensity slider.
Note: The fluorescence channels available depend on the EVOS ™ light cubes
installed in the instrument.
8. When finished, press Done to save the changes to count parameters and return
to the Advanced ™ screen.
Press Cancel to return to the Results screen without saving the changes.
9.
On the Advanced
™
screen, press Count to recalculate your results with the new
count parameters.
10.
To save the changes to size, brightness, or circularity parameters to the current
profile or to create a profile with the new count parameters, see “Save as new
protocol“ on page 39.
Chapter 5 Fluorescence assays
Gate count results
5
38 Countess
™
II FL automated cell counter User Guide