Thermo Scientific Invitrogen Countess II FL, Руководство пользователя

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Cell count and cell viability assays
Count cells in brightfield
1. Prepare the sample by adding 10 µL of your cell suspension to 10 µL of 0.4%
trypan blue stain. Mix the sample mixture well by pipetting up and down a few
times.
2. Load 10 µL of the sample mixture per chamber into the sample slide as described
in “Load Countess
™
chamber slide“ on page 19. Let the sample mixture settle for
30 seconds.
3. Optional: Press the Profiles button and load the desired profile as
described in “Load a profile“ on page 16.
4. Insert the sample slide into the slide port (“Instrument exterior components“ on
page 11), making sure that the sample side is inserted completely into the
instrument. You will hear a soft click, if the slide is pushed in correctly.
5. When the slide is inserted, the instrument automatically illuminates the sample,
sets the intensity of brightfield illumination, and auto focuses on the cells.
Note: To turn off the Auto Lighting function, see “Define count parameters for
the fluorescent channels“ on page 17.
6. Optional: To manually adjust the focus, press the Focus ™ button, and then use
the Focus ™ slider to bring your sample into focus as described in “Set nominal
focus“ on page 57.
4
Capture and count
Countess
™
II FL automated cell counter User Guide 23