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7.
Press the Set button to set the focus and collapse the focus controls.
Once the focus has been set, the Set button on the focus slider becomes inactive,
confirming that the focus setting has been stored.
Note:
If needed, Zoom in on the image to adjust focus or lighting.
8.
Optional: Set exposure using the light source slider.
The light source slider controls the LED intensity, camera gain, and exposure
time and allows you to adjust the image brightness.
Note:
If your instrument is equipped with an EVOS
™
light cube, first press
Adjust, and then select brightfield (white circle) as the light source. Set the
exposure, then press Done to return to the Capture screen.
9.
Press Capture.
Note:
If your instrument is equipped with an EVOS
™
light cube, make sure that
only the BF (brightfield) checkbox is selected under Collect channels before
capturing the image.
The instrument temporarily captures the image and displays the results (total
concentration, percentage and concentration of live and dead cells). For more
information, see “View results“ on page 25.
Chapter 4
Cell count and cell viability assays
Count cells in brightfield
4
24
Countess
™
II FL automated cell counter User Guide
Содержание Invitrogen Countess II FL
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