Thermo Scientific Invitrogen Countess II FL, Руководство пользователя

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Define count parameters for the fluorescent channels
1. To define the new count parameters for fluorescence assays (available only for
instruments equipped with the optional EVOS ™ light cubes):
a.
From the FL selection box, select the desired channel using the
corresponding radio button.
The available options are BF (brightfield) and up to two fluorescence
channels, depending on the light cubes installed (GFP and TxRed in the
following example).
b.
Define the new count parameters for the selected channel using the
corresponding parameter slider (“Count parameters“ on page 15).
Parameter sliders in the BF channel allow you to gate count results based on
size, brightness, and circularity.
The parameter slider in the selected fluorescence channel allows you to gate
count results based on relative fluorescence intensity in that channel.
c.
Repeat for the remaining channels, as necessary.
Note: You can further adjust the size, brightness, or circularity parameters
for the selected profile before or after performing a cell count, as needed.
You can then save these additional changes to the count parameters to the
current profile or as a separate profile directly from the Advanced ™ screen
(see “Save as new protocol“ on page 29)
Chapter 3 Getting started
Load profile
3
Countess
™
II FL automated cell counter User Guide 17