Thermo Scientific HistoStar, Руководство оператора

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HistoStar Operator Guide A81010100 Issue 2
88
Poor Sectioning
Problem Possible Cause Remedy
Poor Sectioning Wax tank too hot Check the melting point of the wax and reduce the
temperature setting as required - see Temperature Set Points.
Contaminated or
inappropriate wax
• Drain the wax tank - see Draining the Wax Tank
• Clean the wax tank - see General Instrument Cleaning
• Refill the wax tank - see Filling with Wax
Flow of wax into
mould is too fast
causing trapped
bubbles
Reduce the wax dispense flow - see Dispense Lever Adjustment
and Wax Flow Control.
Surface of block is
uneven due to mould
being too cold
Use the mould storage area to pre-warm the moulds prior to
use.
Surface of block is
uneven due to
unclean mould
Ensure the moulds are clean before use.
Tissue storage tank is
too hot Check the melting point of the wax and reduce the
temperature setting as required - see Temperature Set Points.
Cross contamination
of wax in wax tank -
mixing of different
waxes
• Drain the wax tank - see Draining the Wax Tank
• Clean the wax tank - see General Instrument Cleaning
• Refill the wax tank - see Filling with Wax
Layers of wax in block Block left on cold-spot for too long after tissue orientation,
before being topped up with wax.
Block does not fit in
microtome chuck due
to excessive wax
residue
Ensure blocks are trimmed properly using the Paratrimmer.
Block breaks in chuck Cassette positioned incorrectly in mould during embedding
Insufficient support
for tissue caused by
mould being too small
for the sample
Use a larger mould