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Incubator BB 150 Operating Instructions

Delivery

2.1

Packaging

The CO

incubator BB 150 is delivered in a stable packaging box. All packaging

materials can be separated and are reusable:

Packaging materials

• Packaging carton

Recycled paper

• Foam elements

Styrofoam (CFC-free)

• Pallet

Untreated wood

• Packaging film

Polyethylene

• Packaging ribbons

Polypropylene

2.2

Components standard equipment

2.3

Acceptance inspection

After the device has been delivered, check the delivery immediately for:
• completeness,
• possible damages.

If damages are detected or if components are missing, please contact the
carrier and Thermo Fisher Scientific Products immediately.

Delivery

incubator with

single glass door and with

continuous shelves

(standard version)

Shelf

Shelf support post

Shelf support rail

Insert for pressure compensation
opening

Plug for pipe channel

Power supply cable

Connector, potential-free contact

Spare caps, set

connecting hose set

Immersion water pump

Open-end wrench,
24 mm

Allen wrench 2 mm for blower
wheel

Allen wrench 3 mm for blower
wheel cover

Operating instructions

Содержание BB 15

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Страница 2: ...he written consent of Thermo Fisher Scientific This regulation does not apply to reproductions for in plant use The contents of the operating instructions are subject to change without further notice...

Страница 3: ...5 Stacking 15 3 6 Modifications 15 4 Description of the device 16 4 1 Front view 16 4 2 Rear view 17 4 3 Safety devices 18 4 4 Work space atmosphere 18 4 5 ContraCon decontamination routine 19 4 6 Sen...

Страница 4: ...ntamination routine 52 9 5 Cancelling the ContraCon decontamination routine 52 10 Maintenance 53 10 1 Inspections and checks 53 10 2 Service intervals 53 10 3 Preparing the temperature calibration 54...

Страница 5: ...13 Center strut gas humidification installation 25 Fig 14 Gas pressure hose installation 26 Fig 15 Power supply connection 27 Fig 16 RS 232 interface connection 28 Fig 17 Example of a connection sche...

Страница 6: ...al notes The following are Thermo Fisher Scientific service contact information For customers in China Tel 800 810 5118 Fax 010 51219151 Website http thermofisher com For customers outside of China Pl...

Страница 7: ...ures are to be taken in case of an accident Any repairs to the device must only be performed by adequately trained and authorized expert personnel The contents of the operating instructions are subjec...

Страница 8: ...instructions WARNING is used if non observance may cause serious or even lethal injuries CAUTION is used if non observance may cause medium to minor injuries or damage NOTE is used for applicational...

Страница 9: ...CO2 Incubator BB 150 Operating Instructions 9 1 General notes 1 3 2 Symbols on the device CE symbol VDE safety tested Test certificate for USA Canada Observe operating instructions...

Страница 10: ...rom a separate gas supply system either from gas cylinders or from a central pressurized gas con tainer The layout of the gas supply system must ensure that the operating pressure of the gas supply li...

Страница 11: ...rved when the CO2 incubator is started up and when the device is operated NOTE Personnel instruction Personnel operating devices with a CO2 sup ply must be instructed about the requirements for the ha...

Страница 12: ...d check the delivery immediately for completeness possible damages If damages are detected or if components are missing please contact the carrier and Thermo Fisher Scientific Products immediately Del...

Страница 13: ...ive heat should not be used near the location of the CO2 incubator BB 150 3 2 Room ventilation When CO2 is supplied the work space of the incubator is slightly pressurized The pressure is released thr...

Страница 14: ...talled near the floor The height of the floor stand should not be less than 200 mm Several floor stands and carriers are available as options from Thermo part numbers see Section 11 1 Spare parts and...

Страница 15: ...the stacked incubator function must be activated on the upper unit This function adjusts the parameters of the uppper unit to compensate any heat trans mission between the two units NOTE Transporting...

Страница 16: ...r switch 6 Pressure compensation opening with insert 7 Measuring opening 8 Outer door 9 Door handle 10 Outer door seal replaceable 11 Stand height adjustable 12 Nameplate 13 Power switch 14 Support ra...

Страница 17: ...150 Operating Instructions 17 4 2 Rear view 1 Stacking elements 2 Pressure compensation opening 3 Access port 42 mm 4 Switchbox with supply interfaces 5 CO2 gas container 4 Description of the device...

Страница 18: ...heating and the additional se parate heating of the outer door minimize the risk of condensate formation on the side walls at the ceiling of the work space and at the glass door Relative humidity The...

Страница 19: ...4 6 Sensor system Fig 6 The blower wheel and two sensor modules are integrated in the baseplate 1 of the measuring cell Sensor 2 for the measurement of the work space tempe rature and of the thermal p...

Страница 20: ...device through a separate con necting sleeve 1 The process gas must be supplied to the device at a fixed pressure that has been preset within a range of 0 8 1 0 bar this must remain unchanged Before t...

Страница 21: ...Any embossings have a large radius As an option the interior container the shelf system and the blower wheel with its cover can be made of copper material NOTE Oxidation of copper components When expo...

Страница 22: ...d into the work space of the device A pressure compensation opening with insert 2 at the rear panel of the device allows a compensation between the pres sures in the work space and in the operating ro...

Страница 23: ...elves gas humidification work space surfaces work space seals and gaskets glass door NOTE Decontamination For details about the cleaning and disinfection of the device see Section 9 5 2 Mounting the d...

Страница 24: ...The support rails are held at the sides by embossing 2 and 5 and secured by the embossing 1 and 6 The sup port rails marked with are inserted at the rear panel of the device with the locksprings 3 fac...

Страница 25: ...nel of the device The tilt protection 5 is also used as a guide for the shelf 2 Slightly raise shelf so that the withdrawal stop 6 can be routed over the shelf supports 3 Make sure that the shelf supp...

Страница 26: ...not be changed NOTE Pressure compensation opening To ensure permanent pressure compensation the pressure compensation opening must not be connected to an exhaust air system The pipe of the pressure co...

Страница 27: ...eaker G 16 Connection to the power supply system 1 Before connecting the device to the power supply check to see if the voltage of the power supply corresponds with the specifications on the nameplate...

Страница 28: ...must be configured as follows 9600 baud 8 data bits 1 stop bit no parity Command sequences Data communication is achieved with a defined structure of command sequences frames Frame structure STX comma...

Страница 29: ...eturned response is incomplete or faulty the CPU responds with an NAK 15H only 1 byte without frame Otherwise the command code with pertai ning length information is regarded as a response and the dat...

Страница 30: ...an alarm mes sage is issued to the connected alarm monitoring system The potential free con tacts 1 changeover contact have been laid out for the following circuits Alarm relay NOTE Switching structu...

Страница 31: ...connecting cable is comprised in the scope of delivery The values for the operating voltage of the external circuits and of the fusing of the alarm system are given in the table above 1 Connect the in...

Страница 32: ...ys for selecting functions and for entering data 4 LEDs that show functions or operating states 1 Key for setting temperature nominal value 2 Key for increasing value 3 Key for reading failure codes s...

Страница 33: ...utine is being run The temperature display shows a three digit number for the corre sponding assembly parameter set P 1 Operating and display board P 2 Measuring cell P 3 Main board P n Parameter numb...

Страница 34: ...To increase the nominal value Press the keys To reduce the nominal value Press the keys 3 Accept and store the nominal value Release both keys The temperature display shows the current actual value m...

Страница 35: ...t This function adjusts the parameters of the uppper unit to com pensate any heat transmission between the two units 1 Activate the stacked incubator mode Keep the key depressed for 5 seconds then rel...

Страница 36: ...curacy of the CO2 measuring system is maintained the de vice should always be started using the auto start routine after the nominal temperature setting has been changed by more than 1 C or after exte...

Страница 37: ...ed for 5 seconds The auto start LED flashes 4 Close all device doors The temperature display shows the actual value the CO2 display shows run the door LED goes off 5 Cancel the auto start routine Keep...

Страница 38: ...e available when contacting Technical Service NOTE Response delay To prevent short term changes of the opera ting conditions from resulting in repeated fai lure messages during the operation of the in...

Страница 39: ...If no failure is detected each display shows three hyphens If the system detects a failure the display that is assigned to the corre sponding control circuit shows a failure code Example If the tempe...

Страница 40: ...The temperature display shows the last 10 failure codes that had been registered The CO2 display shows the number of the individual failure code within the table 6 Exit the failure code table Release...

Страница 41: ...Doors open for m ore than 10 m in ute s doo r switch Clo se device doo rs tes t door switch for correct function 100 Te mpe rature below no minal value Actual value nom inal va lue 1 C Contact Servic...

Страница 42: ...power switch NOTE Thermal protection When the cause of the failure e g excessive temperature in the operating room has been repaired the device is set to normal incubation operation after it has been...

Страница 43: ...components must be installed safely NOTE Hygiene regulations Prior to any operation the user must clean and disinfect the work space in accordance with the hygiene regulations set forth by the ope rat...

Страница 44: ...nominal values for temperature and CO2 content on the operating panel 5 Ventilate work space by leaving both device doors open until acoustic alarm sounds 6 Start device using auto start routine 7 Clo...

Страница 45: ...essories from the work space 2 Pump water out off the water reservoir 3 Clean and decontaminate the work space and wipe device dry 4 Turn device off using the power switch 5 Unplug power connector and...

Страница 46: ...ied out in three stages Predisinfection cleaning final disinfection Recommended cleaning and disinfection agents CAUTION Incompatible cleaning agents Some device components are made of plastic Solvent...

Страница 47: ...isinfectants ZH 1 598 Thermo Fisher Scientific Products recommend the disinfectant Barrycidal 36 When applied properly Barrycidal 36 is a highly effective broad range disinfectant The effectiveness of...

Страница 48: ...s obser ve the safety instructions and hygiene regulations Wear safety gloves Wear safety glasses Wear mouth and respiratory system protec tion gear to protect the mucous membranes Observe the safety...

Страница 49: ...baseplate of the measuring cell The wheel and the cover can be autoclaved Removing blower wheel and cover 1 Fig 21 Remove the two retaining screws 3 of the cover using the supplied Allen wrench 3 mm...

Страница 50: ...urn the device on using the power switch 4 Activate decontamination routine see table in Section 9 4 5 After the decontamination routine has been completed remove the remaining water using a sterile c...

Страница 51: ...run time approx 14 hours The device cools down until the originally set temperature nominal value is reached Postheating phase Remaining run time approx 3 hours During the postheating phase condensat...

Страница 52: ...acoustic alarm sounds after 30 seconds 3 Start the ContraCon routine Keep the key depressed for 5 seconds The ContraCon routine LED flashes 4 Close the device doors The actual value is shown at the te...

Страница 53: ...heck in accordance with the relevant national regulations e g VBG 4 NOTE Functional check If safety devices were removed or disabled for inspections the device must not be operated before the safety d...

Страница 54: ...mparison measurement NOTE Isothermal container Do not use a container filled with water as an isothermal container as the evaporation of water will result in a lower temperature reading Comparison mea...

Страница 55: ...e key The preset value of 37 C is displayed 3 Enter the measured value destination value Press the keys or Press the keys Destination value e g 36 4 C 4 Accept the destination value Press the key The...

Страница 56: ...ough the sealable mea surement opening of the glass door The comparison measu rement must be performed when the device is completely stable Comparison measurement procedure 1 Turn device on using powe...

Страница 57: ...the key The set nominal value of 5 is displayed 3 Enter the measured value destination value Press the keys or Press the keys Destination value e g 5 6 4 Accept the destination value Press the key Th...

Страница 58: ...to the stop 7 Install retainer 1 Procedure for gas supply sterile filter 8 Connect gas hose to sterile filter sleeve and secure it using hose clamp Check to see if the gas hose is securely seated on...

Страница 59: ...n the retaining slot No tools are required to replace the seal 1 Fig 27 Pull magnetic seal 3 out of the guide slot 1 2 Position new seal at a corner 2 and press seal retaining rail 4 into slot 3 Make...

Страница 60: ...Height 780 mm CSP 50051436 Roller set for floor stand set of 4 Guide rollers CSP 50052528 Spare caps Set CSP 50052958 Outer door magnetic seal 637 x 858 CSP 50078769 Glass door silicone seal CSP 5004...

Страница 61: ...ssure compensation opening CSP 50070316 Surface disinfectant 250 ml spray bottle Barrycidal 36 CSP 50052425 Surface disinfectant 500 ml refill bottle Barrycidal 36 CSP 50051939 Gas cylinder monitor GM...

Страница 62: ...3 Temperature control range C RT 3 55 Temperature deviation time DIN 12880 Part 2 C 0 2 Temperature deviation spatial DIN 12880 Part 2 at 37 C C 0 5 Duration of the auto start routine to 37 C ambient...

Страница 63: ...ds to 95 of initial value min 12 Accuracy absolute CO 0 3 Rated voltage V 1 N PE 230 V AC Rated frequency Hz 50 60 Interference suppression DIN VDE 0875 Interference level N Type of protection DIN 40...

Страница 64: ...CO2 Incubator BB 150 Operating Instructions 64 12 Technical data Fig 28 Overview of gas consumption CO2 Fig 28 Overview of gas consumption CO2...

Страница 65: ...ion components Polystyrene foam EPS PPS Compound Printed circuit boards Enclosed electrical components coated with different plastics equipped on epoxy resin bound boards Plastic components general No...

Страница 66: ...Thermo Electron LED GmbH has contracted with one or more recycling disposal companies in each EU Member State and this product should be disposed of or recycled through them Further information on Th...

Страница 67: ...tant Do not wear protective clothing outside the work area Autoclave or disinfect contaminated devices prior to cleaning Germ contaminated waste must be collected safely and destroyed by autocla ving...

Страница 68: ...idemic Act and Livestock Epidemic Germ Di rective is required Pregnant women and breast feeding mothers must not handle infectious hu manopathogenic biological agents or materials containing these age...

Страница 69: ...ns 69 16 Anhang 15 Device log NOTE Device log Record nameplate information work carried out maintenance work and repairs here Device type Part number Serial number Service number Location Operator s n...

Страница 70: ...CO2 Incubator BB 150 Operating Instructions 70 16 Certificate of decontamination...

Страница 71: ...sher Scientific Inc and its subsidiaries Specifications terms and pricing are subject to change Not all products are available in all countries Please consult your local sales representative for detai...

Страница 72: ...range C RT 3 55 Temperature deviation temporal DIN 12880 Part 2 C 0 2 Temperature deviation local DIN 12880 Part 2 at 37 C at 55 C C C 0 5 0 6 Temperature recovery time at 37 C door open 30 s to 98 o...

Страница 73: ...of initial value min 12 CO2 measuring cell Accuracy after auto start routine CO2 0 3 Electrical system Rated voltage V 1 N PE 230 V AC Rated frequency Hz 50 60 Interference suppression DIN VDE 0875 I...

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Страница 82: ...T REGULATING CIRCUIT INCUBATION MODE 24 4 7 4 CO2 METERING AND REGULATION CIRCUIT 25 4 7 5 ERROR IN CO2 METERING AND REGULATION CIRCUIT 26 4 7 6 TEMPERATURE MEASUREMENT REGULATING CIRCUIT DECONTAMINAT...

Страница 83: ...egulating circuits Temperature measurement Temperature regulation Incubation Decontamination routine Sample protection function Plausibility test of the temperature signal with a second digital temper...

Страница 84: ...slaves perform the input output and measurement tasks on site Thus one P controls the display on the unit door while another one on the sensor board controls the fan and generates measured values for...

Страница 85: ...Name Date Designation Prepared JF 05 07 13 Service Manual BB 150 Index Name Date Document No Page File 50078923_04_CN 50 079 823 4 4...

Страница 86: ...emperatures exceeds a range that can be separately defined for the incubation and decontamination modes the entire system is completely shut down In such cases the user can assume that the problem is...

Страница 87: ...uators are located on the main PCB CO2 solenoid activation The 12 VDC CO2 solenoid is connected directly to the board Sample protection In case of overtemperature a special software routine linked to...

Страница 88: ...he door remains open for longer than 30 sec to indicate that the various time locked routines can be selected In addition the horn on the main PCB is activated when a fault is detected The horn signal...

Страница 89: ...ustomer enter incorrect adjustments via the cal key the unit can be reset refer to the adjustment description Faults and errors that arise and are detected during operation are stored in the form of e...

Страница 90: ...he 8 digit check Options that are not configured do no light The version information of the various software versions is then displayed P 1 operator and display PCB e g 010 Version 3 P 2 sensor PCB e...

Страница 91: ...by the cal key refer to the operating instructions 1 To access the function levels described below simultaneously press the cal key the i key and the auto start key and hold them down for at least 5...

Страница 92: ...0 Temperature offset value sign 2 0 0 99 9 Offset value temperature in C 3 0 1 Temperature amplification pre decimal places 4 000 999 Temperature amplification decimal places 5 0 1 cal factor pre deci...

Страница 93: ...ption 1 0 1 Heater actuator unit body X5 X8 2 0 1 Heater actuator unit door X3 3 0 1 Heater actuator base X10 4 0 1 Actuator CO2 valve JP5 5 0 1 Actuator common alarm X11 6 0 1 Actuator horn 7 0 1 Act...

Страница 94: ...ne 5 CO2 countervoltage values FL position FL value range Description 1 0 255 Countervoltage value A NV RAM on the sensor board 2 0 255 Countervoltage value B NV RAM on the sensor board 2 0 255 Counte...

Страница 95: ...ing factor Factory setting Door factor ON PH1 May not be changed When replacing the main board the door heating factor for the model in question must be checked in function level 8 position 2 Readjust...

Страница 96: ...Manual BB 150 Index Name Date Document No Page File 50078923_04_CN 50 079 823 15 15 11 Not reserved FL position FL value range Description 12 Not reserved FL position FL value range Description 13 No...

Страница 97: ...Memory management FL position FL value range Description 1 0 1 Mirror NV RAM on the main PCB 2 0 1 Write default values to measuring cell 3 0 1 Write default values to IRCO2 measuring cell 4 0 1 Upgr...

Страница 98: ...ure flask rotator 7 0 Standard TCD Configure installed measuring cell 8 0 Not installed Configure water level monitor 9 1 Not installed Configure low humidity option 10 0 Not installed Gas guard CO2 1...

Страница 99: ...ior fittings material 0 230 Volt 4 120 Volt Mains voltage 0 Not installed 8 Installed Gas tight screen 0 No O2 O2 0 Not installed Flask rotator 0 Standard Installed measuring cell 0 Not installed Wate...

Страница 100: ...rupted 66 Deviation between temperature probe PT1000 and LM 75 is too large No longer plausible The validity of the temperature signals is no longer assured because the permissible deviation between t...

Страница 101: ...Name Date Designation Prepared JF 05 07 13 Service Manual BB 150 Index Name Date Document No Page File 50078923_04_CN 50 079 823 20 20 measuring cell defective...

Страница 102: ...B both in working order Switch the unit off Disconnect the bus cable to the display PCB at the main board Attach the test set cable display PCB Switch the unit on Perform the functional test Test set...

Страница 103: ...the individual installed components Replace the cable or sensor block Test set not operational Replace the main board 99 Ohmmeter The door switch contact is closed when the glass door is open Perform...

Страница 104: ...based temperature regulator with PT1000 as the temperature sensor integrated in the sensor block P based sample protection function with an upper limit band value that serves as the trigger threshold...

Страница 105: ...he sensor block Test set not operational Replace the main board 100 Heater on LED continuously lit Wattmeter Perform the functional test of relay K1 on the main board Switch the unit off and on Check...

Страница 106: ...or infrared IR sensor is integrated in the sensor block CO2 regulation is P based CO2 gas input is metered by a solenoid The gas flowrate is also restricted by a capillary opening with a diameter of 0...

Страница 107: ...through the solenoid Check the tubing to the detector Adjust the correct initial gas pressure Replace any defective parts Replace the main board as required 201 CO2 Gas off LED always off Initial gas...

Страница 108: ...phase water will condense on the floor of the sample chamber as it cools Cool down phase The unit cools down naturally to the specified set incubation temperature During this phase the exterior door i...

Страница 109: ...on routine that the unit is currently going through The phase is shown on the CO2 display Simultaneously press and hold down the 90 C and i keys for approx 5 seconds S Warm up phase S Decontamination...

Страница 110: ...r code Unit display Test equipment Test at the unit Inspections and tests Possible corrective actions Heater on LED continuously lit Watt meter Perform the functional test forrelay K1 on the main boar...

Страница 111: ...ber heater actuators heaters E2 E8 Check the unit version Replace the main board Return to factory for repairs Set correct set of parameters FL 21 Heavy condensation on the glass Check the door heater...

Страница 112: ...is adjusted to the various operating modes by pulse package control Incubation mode Low speed Decontamination mode Maximum speed The fan runs continuously After a mains reset or when the door is clos...

Страница 113: ...mains power The I C bus system is self configuring that is no adjustments are required Switch the unit on close the glass door prevent the unit from heating up check the operation of the fan Units eq...

Страница 114: ...will reset any cal adjustments that may have been performed The unit is now operating with its original specified adjustment values In units equipped with copper interior fittings set the door factor...

Страница 115: ...rating temperature e g 37 0 C Make sure the unit has adequate time to reach equilibrium before taking a reading For cold units No sooner than 5 hours after the temperature has reached equilibrium or t...

Страница 116: ...nt Adjustment of the CO2 Metering System Comparative measurement CO2 regulating circuit Perform the comparative measurement at the user s normal CO2 concentration e g 5 0 Make sure the unit has adequa...

Страница 117: ...ium at 90 C For units at operating temp No sooner than 45 min after probe placement Comparative measurement procedure Calibrated thermometer 90 0 C 1 C Place the probe in the center of the sample cham...

Страница 118: ...on for approx 15 sec 8 digit test Software versions and parameter set are shown Setpoint specification Temperature setpoint adjustment Display temperature setpoint Press C Current setpoint is displaye...

Страница 119: ...t flashes Please note the duration Activate equalization function See operating instructions Activate other functions Go to the associated function levels via the control panel see Chapter 4 Close all...

Страница 120: ...Index Name Date Designation Document No Page JF 05 07 13 Service Manual BB 150 50 079 823 1 1 50078923_06_CN 6 MEASUREMENT TEST LIST BB 150 Contents page Resistors tests BB 150 2 Voltage tests 3 Safet...

Страница 121: ...g and measure the resistance Meter 2458 2846 E5 Sample chamber rear wall 230 V Units Cu and VA Disconnect the plug and measure the resistance Meter 2294 2656 E6 Sample chamber front side and top 230 V...

Страница 122: ...t voltage X2 2 X2 3 Disconnection the plug and measure the voltage Meter 230 VAC Value voltage JP5 1 JP5 2 Loosen the connection and measure the voltage Meter 12 VDC SAFETY TESTS refer to the circuit...

Страница 123: ...e Date Designation Document No Page JF 05 07 13 Service Manual BB 150 50 079 823 4 4 50078923_06_CN Labels signs nameplate Visual inspection Present and undamaged 1 Applicable documents Procedure inst...

Страница 124: ...LOW CORE SCREW GLASS DOOR HERACELL Hohlschraube M4 f r Glast rscharn X CSP 50050216 HOSE PASS THROUGH WITH SEAL ID 16X90 ROHRDURCHFUEHRUNG ID 16X90 MIT DICHTUNG X CSP 50050438 Door switch T rschalter...

Страница 125: ...Motor X CSP 50068686 Cover plug unit door HERAcell 240 top Blindstopfen Ger tet r oben HERAcell 240 X CSP 50070610 Silicon seal for glass door T rdichtung X CSP 50074739 Plug with sinter metal filter...

Страница 126: ......

Страница 127: ...4 Set value error Error in the calculation of the ser values The processor performs a reset 55 I C bus error Data transfer to the I2 bus interrupted 66 Deviation between temperature probe PT1000 and L...

Страница 128: ...ture above set value Actual value set value 1 0 C Sample protection function active 104 Temperature probe PT1000 or digital temperature sensor LM 75 defective Probe break or sensor short circuit 200 C...

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