6-16HS
6 Using the centrifuge
30
Version 06/2012, Rev. 1.0 • sb-kue
Translation of the original operating manual
• When using smaller bag systems or in case of incompletely filled blood
bags adapters (e.g. part no. 17750) have to be inserted together with
the blood bag systems. This will avoid slipping of the bags which could
result in an imbalance.
• For the centrifugation of human blood suitable acceleration and
deceleration curves must be used. Curve no. 12 is the most suitable for
this purpose.
A German blood bank works successfully using the following data:
1
T
e
m
p
e
ra
tu
re
1
)
S
p
e
e
d
R
C
F
T
im
e
t
A
c
c
e
le
ra
ti
o
n
c
u
rv
e
2
)
D
e
c
e
le
ra
ti
o
n
c
u
rv
e
P
ro
g
ra
m
°C
rpm
x g
min
no.
no.
22
3,750 4,544
7
20 (2 min)
12
1 Full blood
22
3,570 4,544
10
20 (2 min)
12
2 Full blood in 4fold bags
20
4,000 5,170
12
24 (3 min)
12
3
Plasma with buffy coat
22
3,750 4,544
9
20 (2 min)
12
4 Full blood
20
4,000 5,170
14
24 (3 min)
12
5 Plasma with buffy coat
22
1,094
384
7
21 (2 min)
11 or 2 min
6 Platelets with buffy coat
22
1,094
384
7
21 (2 min)
11 or 3.5min 7 Platelets with buffy coat
1)
only with special equipment heater
2)
Curves no. 20, 21 and 24 are freely programmable curves
NOTE
If the centrifuge does not reach the mentioned RCF values, the maximum
gravitational field must be set and the run time must be extended.
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6.2.2.7
Centrifugation with tubes
In the ASTM standard, a maximum gravitational field of 800 x g is required
for the centrifugation with tubes for mineral oil. Practically, it is possible to
reach higher RCF-values with the rotor 11165 and the buckets 13113,
13114 or 13116.
CAUTION
The required gravitational field must not be exceeded.
Values above 800 x g increase the risk of glass breakage!
This is why a program is stored in the system as a default setting of the
centrifuge. This program is specifically adapted to the petroleum rotor
11165 with buckets of the 13113 type.
• Use sealing caps (part no. 17107).
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