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6-16HS 

6 Using the centrifuge 

 

 

 

Version 06/2012, Rev. 1.0 • sb-kue 

29 

Translation of the original operating manual 

 

Pos: 112  /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060  Betrie b_Spinc ont rol L +S/06 0-0 020 -00 20- 004 0   Ad apte r -  nur  L+S @  7\ mod _130 640 367 744 1_6 8.doc x @ 4 359 7 @  44 @  1 

 

6.2.2.4  Adapters 

 In order to ensure easy handling, even if vessels of various sizes are used, 
carrier systems were developed. 

•  Load the opposite carriers with the same number of vessels and with 

the same weights in order to avoid imbalance. 

•  If all of the compartments of a carrier are not used, the buckets must be 

loaded evenly. Loading the edges of a bucket only is not permissible. 

Pos: 113  /01 0 Univ ersal mod ule/                                                             Lee rzeile  @ 0\ mo d_12 021 162 445 00_ 0.doc x @ 1 14 @   @ 1 

 

 

Pos: 114  /10 0 Sigm a/10 0 BA Z entrif uge n (St anda rd mod ule)/ 060  Betrie b_Spinc ont rol L +S/06 0-0 020 -00 20- 005 0   G efä ße @  7\m od_ 130 640 392 328 3_68 .docx  @ 4 360 4 @ 4 4 @  1 

 

6.2.2.5  Tubes 

•  Load the tubes outside of the centrifuge. Liquids in the buckets or 

multiple carriers cause corrosion . 

•  Fill the tubes carefully and arrange them according to their weight. 

Imbalances result in the excessive wear of the bearings. 

•  In high-speed angle rotors, the vessels must be filled up to their useful 

volume (= the volume stated for the vessel). If the vessels are only 
partially filled, they will deform. This may result in leaks at the seals that 
may become loose. 

•  When using glass tubes, the maximum value of 4,000 x g must not be 

exceeded (except special high-strength glass tubes; please refer to the 
information provided by the manufacturer). 

•  Follow the safety instructions and hazard warnings (see chapter 3 - 

"

Safety

")! 

Pos: 115  /01 0 Univ ersal mod ule/                                                             Lee rzeile  @ 0\ mo d_12 021 162 445 00_ 0.doc x @ 1 14 @   @ 1 

 

 

Pos: 116  /10 0 Sigm a/11 0 BA Z entrif uge n (Pr ojekt e)/6 -16S _6- 16HS/06 0 Bet rieb/ 060 -00 20- 002 0-0 060 Blu tbeu telsyst eme  @ 1 3\m od_1 340 607 136 850 _68. docx  @ 67 825  @ 44  @ 1 

 

6.2.2.6 

Blood bag systems 

The centrifuge tolerates an imbalance of approx. 20 g. Therefore, it is 
important to balance the buckets carefully. 

•  All four places of a rotor must be loaded with buckets. It is permissible 

to fill just two opposite buckets with blood bag systems. 

•  The opposite buckets including the filled blood bag systems must have 

an equal weight. 

•  The blood bags have to be put into the opposite buckets mirror-inverted. 

•  If in one bucket the main blood bag is on the left side, then the main 

blood bag inserted in the opposite bucket has to be on the right side. 

 

 

1  Filter 
2

Blood bag

 

  

 

 

 

Fig. 9: Loading of blood bag systems 

Содержание 6-16HS

Страница 1: ...ual Laboratory Centrifuge with Heating System 6 16HS Spincontrol S Translation of the original operating manual sb kue Version 06 2012 Rev 1 0 6 16HS Spincontrol S From serial no 145877 Please retain...

Страница 2: ...6 16HS 2 Version 06 2012 Rev 1 0 sb kue Translation of the original operating manual...

Страница 3: ...right by Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode am Harz Germany Tel 49 0 5522 5007 0 Fax 49 0 5522 5007 12 Web www sigma zentrifugen de E mail service sigma zentrifugen de F...

Страница 4: ...6 16HS 4 Version 06 2012 Rev 1 0 sb kue Translation of the original operating manual...

Страница 5: ...afety 14 3 1 Marking of the unit 14 3 2 Explanation of the symbols and notes 15 3 3 Responsibility of the operator 16 3 4 Operating personnel 16 3 5 Informal safety instructions 16 3 6 Safety instruct...

Страница 6: ...angle rotors with a hermetically sealed lid 27 6 2 2 3 Installation of accessories 28 6 2 2 4 Adapters 29 6 2 2 5 Tubes 29 6 2 2 6 Blood bag systems 29 6 2 2 7 Centrifugation with tubes 30 6 3 Contro...

Страница 7: ...ge 55 8 1 6 Condenser only air cooled centrifuges 56 8 2 Sterilisation and disinfection of the rotor chamber and accessories 56 8 2 1 Autoclaving 57 8 3 Service 58 8 4 Return of defective parts 59 9 D...

Страница 8: ...en 010 00 20 Be stim mung sge m e Ve rwend ung 7 mod _130 587 431 359 5_6 8 doc x 4 315 1 2 1 1 2 Intended use Centrifuges are power driven machines that separate liquids from solid matter liquid mix...

Страница 9: ...hrift en 7 m od_ 130 587 4739 801 _68 docx 4 3172 2 1 1 5 Standards and regulations EC Declaration of Conformity see chapter 11 6 EC declaration of conformity Pos 11 010 Unive rsalm odul e L ee rzeile...

Страница 10: ...e 7 mod _13 062 210 423 42_ 68 d ocx 432 01 2 1 2 1 Layout of the centrifuge Pos 17 100 Sigma 110 BA Ze ntrifu gen Proj ekte 6 16S_ 6 1 6HS 020 Aufba u u nd Wirk ungsw eise 020 00 10 001 0 Funktio ns...

Страница 11: ...376 38_ 68 d ocx 684 21 3 1 2 1 2 Name plate 1 Manufacturer and registered office 2 Type 3 Serial number 4 Max speed 5 Max kinetic energy 6 Max density 7 Nominal voltage 8 Input fuse 9 Symbol for spe...

Страница 12: ...he bigger the radius in the rotor chamber is and the higher the speed is the higher the centripetal acceleration is However the forces acting on the rotor also increase Pos 23 010 Unive rsalm odul e L...

Страница 13: ...resulting relative centrifugal force will be recalculated automatically by the control unit If the RCF is changed the speed will be adapted while the specified radius is maintained The speed gravitat...

Страница 14: ...on consulter le mode d emploi I Ein Netzverbindung On Power Marche mise sous tension 0 Aus Netzverbindung Off Power Arr t mise hors tension Schutzleiteranschluss Protective earth ground Raccord la ter...

Страница 15: ...s health problems up to life endangering injuries DANGER This symbol stands for a direct hazard to the life and health of persons due to electrical voltage Non observance of these symbols causes serio...

Страница 16: ...120 211 624 450 0_0 docx 11 4 1 Pos 38 100 Sigma 100 BA Ze ntrifu gen Sta nda rdm odul e 0 30 Sich erh eit 0 30 0040 Bedi enp erso nal 7 m od_ 130 630 324 7689 _68 docx 4 3282 2 2 1 3 4 Operating pers...

Страница 17: ...n Sta nda rdm odul e 0 30 Sich erh eit 0 30 0060 00 20 Mec hanis che Sich erh eit 7 m od_ 130 6302 666 334 _68 docx 43 261 3 3 1 3 6 2 Mechanical safety In order to ensure the safe operation of the ce...

Страница 18: ...0 30 0060 00 40 Che mische un d biol ogisch e Siche rheit 7 mod _13 063 046 0204 4_6 8 d ocx 433 17 33 1 3 6 4 Chemical and biological safety If pathogenic toxic or radioactive samples are intended to...

Страница 19: ...mbalances at high speeds Ensure that rotor and buckets are correctly fitted see chapter 6 2 2 1 Installation of the rotor Observe the instructions on the installation of accessories see chapter 6 2 2...

Страница 20: ...to changes such as corrosion cracks material abrasion etc If other data concerning the service life are engraved on the rotor or bucket these data shall apply accordingly For example a bucket with the...

Страница 21: ..._0 docx 11 4 1 Pos 64 100 Sigma 100 BA Ze ntrifu gen Sta nda rdm odul e 0 30 Sich erh eit 0 30 0070 00 40 Schutzl eiter pr fung Spinco ntr ol L S 7 mod _13 063 0689 173 7_6 8 do cx 4338 7 33 1 3 7 4 E...

Страница 22: ...ul e 0 30 Sich erh eit 0 30 0090 Res trisike n 7 mo d_1 306 307 3267 31_ 68 docx 43 422 2 1 3 9 Remaining hazards The unit was built state of the art and according to the accepted safety rules However...

Страница 23: ...x 4 3443 2 2 1 4 2 Storage conditions The centrifuge can be stored in its original packaging for up to a year Store the centrifuge only in dry rooms The storage temperature must be above 20 C If you w...

Страница 24: ...e ntrifu gen Proj ekte 6 16S_ 6 1 6HS 040 La ger ung und Tr ansp ort 040 00 50 Tra nsp ortsich eru ng 6 1 6S_6 16HS 13 mod _13 4035 885 615 6_6 8 do cx 6 769 6 2 1 4 5 Transport safety device The tran...

Страница 25: ...ee rzeile 0 mod _120 211 624 450 0_0 docx 11 4 3 1 Pos 92 100 Sigma 100 BA Ze ntrifu gen Sta nda rdm odul e 0 50 Auf stellun g u nd Ansc hluss 050 002 0 En ergi ever sor gung 7 mo d_13 063 120 354 39_...

Страница 26: ...t anda rd mod ule 060 Betrie b_Spinc ont rol L S 06 0 0 020 00 10 ffn en u nd Schli e e n d es Deck els 1 Schloss 1 2 mo d_1 336 037 032 225 _68 docx 64 305 33 1 6 2 1 Opening and closing the lid The...

Страница 27: ...f uge n Pr ojekt e 6 16S _6 16HS 06 0 Bet rieb 060 00 20 002 0 0 020 6 1 6S Winkel roto ren mit her metisc h ve rschlie ba re m Deckel 1 3 m od_1 340 359 480 277 _68 docx 67 722 4 4 1 6 2 2 2 Installa...

Страница 28: ...zes Working with different tube sizes is possible In this case however it is very important that opposite inserts are identical see figure Fig 7 Permissible loading of the swing out rotor with differe...

Страница 29: ...In high speed angle rotors the vessels must be filled up to their useful volume the volume stated for the vessel If the vessels are only partially filled they will deform This may result in leaks at...

Страница 30: ...h buffy coat 1 only with special equipment heater 2 Curves no 20 21 and 24 are freely programmable curves NOTE If the centrifuge does not reach the mentioned RCF values the maximum gravitational field...

Страница 31: ...33 1 6 3 1 User interface The centrifuge is operated via three buttons with integrated light emitting diodes and one function knob The display is divided into several different fields The various func...

Страница 32: ...trifuge lid must be opened before a new centrifugation run can be started A quick stop can also be triggered during a normal deceleration e g in order to speed up the deceleration When a quick stop is...

Страница 33: ...of the centrifuge is displayed The actual speed is displayed below this value The values are stated in revolutions per minute rpm and depend on the RCF values see chapter 2 2 2 1 Speed radius and rela...

Страница 34: ...the continuous run until the set speed is reached Select the field Runtime and confirm the selection The display flashes when it is activated Turn the function knob from the time 0 00 10 anti clockwis...

Страница 35: ...ion list Identifying and adapting incorrectly set rotors The centrifuge automatically identifies the rotor that is being currently used If the system identifies a different rotor than the one that is...

Страница 36: ...input The program selection list will be displayed Select the program ASTM oil from the list and confirm the input The following values will be loaded Language English Rotor bucket 11165 13113 RCF 650...

Страница 37: ...The RAPID_TEMP program will be stopped under the following conditions The set value is reached In this case the RAPID_TEMP program stops with a sound signal and the standstill heating system will be a...

Страница 38: ...ion Brake This function is used in order to select a curve that decelerates the centrifuge to a standstill Deceleration curves are inverted images of the acceleration curves and are labelled with iden...

Страница 39: ...quid to be centrifuged is higher than 1 2 g cm3 the value must be adapted manually This will reduce the maximum possible final speed see chapter 2 2 2 2 Density A downward facing arrow will be display...

Страница 40: ...l be decelerated to a complete standstill Spin out from set speed If this function is active the drive will be disconnected if the actual speed is below the set speed As a result the rotor decelerates...

Страница 41: ...135 01 0 Univ ersal mod ule Sei tenwe chsel 0 mod _12 021 1624 431 2_0 docx 1 05 1 Pos 136 10 0 Sigm a 10 0 BA Zentrif uge n St anda rd mod ule 060 Betrie b_Spinc ont rol L S Spinco ntr ol S Zent 7 0...

Страница 42: ...isplay the symbol will be displayed During a deceleration process the symbol flashes Once the deceleration is complete it is displayed in a permanent manner Display Temperature unit Select between C C...

Страница 43: ...ntrifuge will be defined The characters can be entered when the cursor flashes in the text field Turn the function knob in order to select a character and press it to confirm the selection Then press...

Страница 44: ...ssing the function knob again Parameters can be changed when the help function is activated Fig 19 Help menu Pos 139 01 0 Univ ersal mod ule Lee rzeile 0 mo d_12 021 162 445 00_ 0 doc x 1 14 1 Pos 140...

Страница 45: ...60 Betrie b_Spinc ont rol L S Spinco ntr ol S Zent 7 060 00 30 0030 0 010 Prog ra mm la den 1 2 m od_1 336 716 666 809 _68 docx 64 371 44 1 6 3 3 1 Loading a program Select the option Progr from the S...

Страница 46: ...Pos 148 10 0 Sigm a 10 0 BA Zentrif uge n St anda rd mod ule 060 Betrie b_Spinc ont rol L S Spinco ntr ol S Zent 7 060 00 30 0030 0 030 Prog ra mm l sche n 1 2 m od_ 133 671 7133 741 _68 docx 6 4387...

Страница 47: ...elect Program rotation in the Setup menu Fig 24 Program rotation function The current program is loaded and can be started After the end of the run the next program will be loaded and started automati...

Страница 48: ...ve DC 24V 0 5A max part no 17701 Floating switch AC 250V max 6A part no 17702 Pos 153 01 0 Univ ersal mod ule Lee rzeile 0 mo d_12 021 162 445 00_ 0 doc x 1 14 1 Pos 154 10 0 Sigm a 10 0 BA Zentrif ug...

Страница 49: ...vate temperature fuse see chapter 5 2 2 Customer provided fuses Mains power switch off Switch mains power switch on Centrifuge cannot be started start key LED is not illuminated Several Power off on I...

Страница 50: ...he opening on the control panel e g with a screwdriver Fig 26 Position of the opening for the emergency lid release Insert the supplied hexagon socket key horizontally into the hole The key will be gu...

Страница 51: ...gerated centrifuges Allow to slow down Power off Allow to cool down Provide better ventilation only air cooled centrifuges Provide sufficient water throughput only water cooled centrifuges 46 49 Imbal...

Страница 52: ...vice Area or contact Sigma Laborzentrifugen GmbH An der Unteren S se 50 37520 Osterode Germany Tel 49 0 55 22 50 07 84 25 Fax 49 0 55 22 50 07 94 25 E mail service sigma zentrifugen de Outside Germany...

Страница 53: ...iv ersal mod ule Lee rzeile 0 mo d_12 021 162 445 00_ 0 doc x 1 14 33 1 Pos 169 10 0 Sigm a 10 0 BA Zentrif uge n St anda rd mod ule 080 Wart ung und Inst andh altu ng 0 80 001 0 Wa rtu ngsa rbeit en...

Страница 54: ...xic radioactive or pathogenic contamination Dry the accessories with a soft cloth or in a drying chamber at approx 50 C Pos 173 01 0 Univ ersal mod ule Lee rzeile 0 mo d_12 021 162 445 00_ 0 doc x 1 1...

Страница 55: ...n St anda rd mod ule 080 Wart ung und Inst andh altu ng 0 80 001 0 0 040 Trag bolze n 7 mo d_1 306 741 641 932_ 68 docx 43 855 33 33 1 8 1 4 Load bearing bolts Only greased load bearing bolts ensure t...

Страница 56: ...possible Check the condenser at least once a month for dirt and clean it if necessary If you have any queries please contact service see chapter 7 3 Service contact Pos 185 01 0 Univ ersal mod ule Lee...

Страница 57: ...It cannot be excluded that plastic parts e g lids or carriers may deform during autoclaving Accessories Max temp C Min time Max time Max cycles Glass tubes 134 138 3 40 Polycarbonate tubes 115 118 30...

Страница 58: ...rts that are among other things part of the motor suspension are subject to ageing This is why we recommend having the centrifuge checked by the manufacturer during an inspection once per year in the...

Страница 59: ...specialised personnel only Affix the original form in a clearly visible manner to the outside of the packaging NOTE If no such declaration is enclosed in the return package we will perform the deconta...

Страница 60: ...es free of cost to Sigma Laborzentrifugen GmbH Ensure that the unit is decontaminated Fill in a declaration of decontamination see chapter 8 4 Return of defective parts Comply with any other applicabl...

Страница 61: ...106 950 Other parameters Time range Storage locations 10 sec 99 h 59 min short run continuous run 60 Physical data Height mm Height with open lid mm Width mm Depth mm Weight kg 483 990 581 711 116 EM...

Страница 62: ...n For environmental reasons the comprehensive technical documentation of the centrifuge e g circuit diagrams and the safety data sheets of the manufacturers of refrigerant and lubricants are not attac...

Страница 63: ...lar buckets no 13420 4 sets rectangular carriers no 18015 and 96 PPCO tubes 15 ml no 15023 Radius max 19 3 cm radius min 10 5 cm 4 200 3 806 11156 Swing out rotor 6 place for buckets no 13127 11157 Sw...

Страница 64: ...5 2 0 ml Biosafe with certificate reaction vials no e g 15008 15040 incl bayonet lid no 17612 Radius max 10 0 cm radius min 6 5 cm 13 500 20 376 12600 Angle rotor 6 x 500 ml Biosafe with certificate...

Страница 65: ...500 ml bottle no 15501 750 ml bottles no 15751 sealable with cap no 17134 17135 max tube length 155 mm 1 set 2 pcs Radius max 21 4 cm radius min 8 4 cm 4 500 4 845 13555 Adapter for 1 bottle 500 ml m...

Страница 66: ...ture tube 50 ml e g no 15151 suitable for 12165 1 set 2 pcs 13081 Adapter for 1 culture tube 15 ml e g no 15115 suitable for 12165 1 set 2 pcs 13082 Adapter for 1 tube 50 ml max 28 8 x 105 115 mm e g...

Страница 67: ...s please refer to the information provided by the manufacturer When installing the 500 ml bottles use the supplied supporting rings Attention At speeds above 8 000 rpm there is an increased risk of br...

Страница 68: ...10 0 BA Zentrif uge n St anda rd mod ule 110 Anha ng 1 10 002 0 Dr ehz ahl Schw ere feld Di agr am m 7 mo d_1 306 836 022 039 _68 docx 44 018 22 2 1 11 2 Speed gravitational field diagram Fig 30 Speed...

Страница 69: ...ncreasing acceleration from right to left The deceleration curves are inverted images of the acceleration curves and are assigned the same numbers An exception is curve 0 It decelerates brakeless spin...

Страница 70: ...power The runtime depends solely on the moment of inertia of the rotor Quadratic curve no Time until 1 000 rpm Slope as of 1 000 rpm 10 500 sec 4 rpm sec 11 333 sec 6 rpm sec 12 250 sec 8 rpm sec 13 1...

Страница 71: ...00 300 400 500 600 700 800 900 1000 0 100 200 300 400 500 Time sec Speed rpm curve 10 curve 11 curve 12 curve 13 curve 14 curve 15 curve 16 curve 17 curve 18 curve 19 18 19 17 16 15 14 13 12 11 10 Fig...

Страница 72: ...1 14K 12094 5 years 20x 1 14 1 14K 12096 5 years 20x 1 14 1 14K 12101 5 years 20x 1 15 1 15K 1 15P 1 15PK 12124 5 years 20x 1 15 1 15K 1 15P 1 15PK 12126 5 years 20x 1 15 1 15K 1 15P 1 15PK 9100 15 0...

Страница 73: ...1 1 4 1 1 4 4 1 1 Acetone C3H6O 100 1 1 4 1 1 4 4 1 1 Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 1 Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1 Aluminium chloride AlCl3 saturated 1 3 2 4 1 1 1 4 Aluminiu...

Страница 74: ...SO4 x 5H2O 10 1 1 1 1 1 1 1 1 1 4 Cyclohexanol C6H12O 100 1 1 3 1 1 1 1 4 1 1 Decane C10H22 100 1 2 1 3 1 1 Diaminoethane C2H8N2 100 1 1 3 1 1 3 4 1 1 Diesel fuel 100 1 1 3 1 1 1 3 1 1 Dimethyl formam...

Страница 75: ...3 1 3 1 2 1 1 2 1 1 Magnesium chloride MgCl2 10 1 1 1 1 1 1 1 1 1 1 Mercuric chloride HgCl2 10 1 4 1 3 1 1 1 1 1 4 Mercury Hg 100 1 1 1 1 1 1 1 3 1 3 Methyl acetate C3H6O2 100 1 1 4 2 1 4 4 1 1 Methyl...

Страница 76: ...Silver nitrate AgNO3 100 1 1 1 1 1 1 1 1 1 4 Sodium bisulphite NaHSO3 10 1 1 2 4 1 1 1 Sodium carbonate Na2CO3 10 1 1 1 1 1 1 3 Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3 Sodium hydroxide NaOH 30 1 1...

Страница 77: ...Polyethylene Polyamide Polycarbonate Polyoxymethylene Polypropylene Polysulfone Polyvinyl chloride hard Polyvinyl chloride weak Polytetrafluorethylene Aluminium Medium Formula HDPE PA PC POM PP PSU PV...

Страница 78: ...original operating manual Pos 223 10 0 Sigm a 11 0 BA Zentrif uge n Pr ojekt e 6 16S _6 16HS 11 0 Anh ang 110 00 60 Ko nfor mit tserkl ru ng 4 16S 13 mo d_1 340 605 0552 20_ 68 docx 67 817 22 2 1 11...

Страница 79: ...fectious toxic radioactive or pathogenic substances 18 Centrifugation principle 12 Centrifugation with different tube sizes 28 Centrifugation with low capacity 28 Centrifugation with tubes in complian...

Страница 80: ...e substances 18 External 43 F Fire preventions 18 Form for the return of defective parts 59 Function 41 Functional and operating elements 10 Fuses 25 Fuses have tripped 49 G General conditions 8 Glass...

Страница 81: ...n lid after run 41 Opening the lid 26 Operating personnel 16 Operating voltage 25 Operational safety 54 Optical signal 43 Option for the input and output of data 43 Options for data input and output 4...

Страница 82: ...e 53 54 Service life of rotors and accessories 20 72 Service life of the accessories 57 Service request 3 52 58 62 Service work 58 Set up and connection 25 Setup menu 41 Short run 34 Slushing oil 9 Sl...

Страница 83: ...53 Threshold delta T 40 Time fine 42 Time range 61 Toxic substances 18 53 Transport safety device 24 Tubes 29 Type of the centrifuge 52 58 61 U Unblocking a function 41 Ungreased load bearing bolts 4...

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