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OPERATION 

 

CO

2

 Atmosphere 

The microprocessor also controls the gas concentration of CO

2

 in the chamber atmosphere by 

operating an internal injection solenoid valve connected to a gas input port. The processor monitors 
CO

2 

concentration level in the incubator using an infrared sensor located in the recirculation duct. 

The sensor operates on the principle that a specific frequency set of infrared light is absorbed by 
CO

2

. The more CO

2

 present in the chamber, the more of that band of infrared is absorbed. The 

sensor is only sensitive to CO

2

, so measurement accuracy is consistent, regardless of the presence 

of other gases in the incubator. 

The processor employs proportional-integral-derivative (PID) analytic feedback-loop functions when 
measuring and controlling the CO

2

 concentration. Injection lengths are proportional to the difference 

between the measured concentration and the set point. The frequency of injections is derived from 
the rate of change in the difference. Integrator feedback slows the rate of injection as the 
concentration approaches the set point, which helps prevent overshoots. When the chamber 
concentration is stable CO

2

 injections take place in small bursts to correct for deviations less than 

0.1%. The incubator is not provided with a means to actively remove CO

2 

from the chamber 

atmosphere. 

 

Humidification 

Passive humidification is provided by filling the humidification pan included with the unit. The pan is 
then placed on the heated chamber floor. Evaporation driven in part by heating raises the relative 
humidity percentage (RH%) of the chamber. A copper token included with the pan helps to 
significantly slow the growth of microbiological populations in the humidification water supply. 

The incubator must be operated humidified in order to achieve its stated temperature uniformity 
specification. 

 

Physical and Data Access 

An access port on the right side of the unit allows sensors such as thermocouples probes to be 
inserted and left in the chamber without compromising the CO

2

 atmosphere or chamber 

temperature. An atmosphere sample port for independently verifying the CO

2

 concentration in the 

chamber is provided on the left side of the unit, adjacent to the control panel. A USB outputs CO

2

 

and temperature levels once per minute as a serial-protocol digital log line. An adjacent dry-contact 
jack communicates alarm activation incidents. Please see the 

Data Output

 entry on page 41 for more 

details. 

 

The Over Temperature Limit System (OTL) 

When set, the mechanical OTL heating cutoff system prevents runaway heating in the unit chamber. 
The OTL operates independently of the microprocessor controller and connects to a hydrostatic 
temperature sensor probe located in the incubation chamber. In the event the chamber air 
temperature exceeds the current OTL setting, the OTL routes power away from the heating element. 
The OTL will continue to prevent heating until the temperature drops below its limit setting. The Over 
Temperature Limit is set 

by the user

, typically at approximately 1°C above the application 

temperature set point. 

Содержание SCO5W

Страница 1: ...WATER JACKETED CO2 INCUBATOR 110 120 Volts Installation Operation Manual SCO5W ...

Страница 2: ...2 P a g e ...

Страница 3: ...rial or educational use where the preparation or testing of materials is done at an ambient air pressure range of 22 14 31 3 inHg 75 106 kPa with no flammable volatile or combustible materials being heated These units have been tested to the following requirements CAN CSA C22 2 No 61010 1 2012 CAN CSA C22 2 No 61010 2 010 2004 Reaffirmed 2014 07 UL 61010 1 2012 05 UL 61010A 2 010 2002 03 EN 61010 ...

Страница 4: ...t 20 Shelving Installation 21 Access Port Stopper 23 Fill the Water Jacket 23 GRAPHIC SYMBOLS 25 CONTROL PANEL OVERVIEW 27 OPERATION 29 Theory of Operation 29 Put the Incubator into Operation 31 Humidify the Incubator 33 Muting the Audible Temperature Alarm 35 Automatic Door Cutoff 35 Start a Flow of CO2 36 Muting the Audible CO2 Alarm 38 No Gas Supply Alarm 38 Set the Over Temperature Limit 39 Lo...

Страница 5: ...5 P a g e UNIT SPECIFICATIONS 57 Weight 57 Dimensions 57 Capacity 58 CO2 58 Temperature 58 Power 58 PARTS LIST 59 Ordering Parts and Consumables 60 Accessories 60 ...

Страница 6: ...6 P a g e TABLE OF CONTENTS ...

Страница 7: ...perator or their supervisors must draw up a site specific standard operating procedure SOP covering each application and associated safety guidelines This SOP must be written and available to all operators in a language they understand Intended Applications and Locations These incubators are intended for laboratory industrial and educational microbiological cultivation applications These incubator...

Страница 8: ...E 1 800 322 4897 extension 4 or 503 640 3000 FAX 503 640 1366 Sheldon Manufacturing INC P O Box 627 Cornelius OR 97113 ENGINEERING IMPROVEMENTS Sheldon Manufacturing continually improves all of its products As a result engineering changes and improvements are made from time to time Therefore some changes modifications and improvements may not be covered in this manual If your unit s operating char...

Страница 9: ...of CO2 gas pressure at the incubator intake port labeled CO2 to Chamber Two Stage Regulator If connecting to a supply cylinder always use a two stage CO2 pressure regulator During normal operations the incubator uses small quantities of CO2 to maintain the chamber gas concentration Under these conditions precise regulation of the gas input flow is vital for the incubator performance Some single st...

Страница 10: ...g For best CO2 accuracy use a digital gas analyzer with sample tubing connecting to the incubator CO2 sample port The barbed adapter gas fitting on the port connects to 3 16 inch 4 76mm inner diameter ID 5 16 inch 7 94mm outside diameter OD tubing Why Probes and Tubing Reference readings taken outside the chamber using wire temperature probes and CO2 drawn through the sample port avoid chamber doo...

Страница 11: ...o the carrier service that delivered the unit 3 If the carton is not damaged open the carton and remove the contents 4 Inspect the unit for signs of damage See the orientation depiction on the next page as a reference 5 The unit should come with an Installation and Operation Manual 6 Verify that the correct number of accessory items have been included 7 The access and water fill stoppers ship inst...

Страница 12: ...lter Door Latch Viewing Door Glass Chamber Door Back of Unit Fuse in Power Inlet CO2 In Ports Chamber Door Handle Back of Unit Fill Water Jacket Ports Siphon Viewing Door Knob Anode Access Port Door Gasket CO2 Sample and Data Ports Control Panel Side of Unit Shelf Standard Mounting Rail ...

Страница 13: ...data plate contains the incubator model number and serial number Tech Support will need this information during any support call Record it below for future reference The data plate is located on the inside of the chamber door top right corner Model Number Serial Number ...

Страница 14: ...14 P a g e RECEIVING YOUR UNIT ...

Страница 15: ...9 Install the Incubator in a suitable location Review the lifting and handling instructions page 18 Place the incubator in its workspace location page 19 Make sure the incubator is level page 18 Set up the incubator for use Clean and disinfect the incubator and accessories except the HEPA filter that will be placed in the incubation chamber page 19 Do not use deionized water to clean the unit or f...

Страница 16: ...cooling 4 inches 102mm of clearance is required on the sides and back 2 inches 51mm of headspace clearance between the top of the unit and any overhead partitions ENVIRONMENTAL DISRUPTION SOURCES When selecting a location to install the unit look for sources of heat cold and moving air that can affect the chamber temperature and atmospheric integrity Ovens autoclaves and any device that produces s...

Страница 17: ...eparate circuit to prevent loss of the unit due to overloading or circuit failure The recommended wall circuit breakers for these units are 15 amps The wall power source must conform to all national and local electrical codes Power Cord The unit must be positioned so that all users can quickly unplug the cord in the event of an emergency Each unit comes provided with a 115 volt 15 Amp 9 2ft 2 86m ...

Страница 18: ...or stabilization Restrain the unit completely while lifting or transporting so it cannot tip Remove all moving parts such as shelves and trays and lock doors in the closed position during transfers to prevent shifting and damage LEVELING Install the leveling feet in the 4 corner holes on the bottom of the unit The unit must be level and stable for safe operation Note To prevent damage when moving ...

Страница 19: ...ty range of 20 0 uS cm to 1 0 uS cm for cleaning and humidifying and water jacketing applications INSTALLATION CLEAN AND DISINFECT Cleaning and disinfecting the unit incubation chamber shelving components and ceiling air duct now reduces the risk of contamination The chamber and shelving were cleaned and disinfected at the factory however the unit may have been exposed to contaminants during shipp...

Страница 20: ...g on the ceiling air duct The filter should snap into pace It may be necessary to tilt the ring slightly to one side while doing so Attach the black plastic cap to the HEPA filter Failure to do so will allow air to blow around the filter instead of blowing through it Place the duct and filter into the chamber slightly tilted to one side or the other for ease of movement The filter must be toward t...

Страница 21: ...rd rails Align the keyhole slot of the standard with the mounting peg on the side of the chamber wall Mount the shelf standard Install the shelf slides Insert the shelf slide into the shelf standard using a rocking motion The shelf slide will sit level when correctly installed Install the shelves Slide into position Figure 1 Shelving Installation ...

Страница 22: ... Connect the supply source to the incubator If connecting a second supply cylinder do so now Do not initiate a flow of CO2 to the incubator at this time End of procedure CO2 to Chamber IN Connects to 3 16 4 76mm ID Tubing Single Source Dual Source IN Optional Tubing Kit with Inline HEPA Filter ...

Страница 23: ...or CO2 specifications without the stopper installed FILL THE WATER JACKET The water jacket requires 19 gallons 72 liters to provide sufficient thermal insulation for the incubator to operate at its stated temperature specifications The manufacturer recommends the use of distilled water or filtered tap water if possible Please see the Anode and Water Quality entry in the maintenance section on page...

Страница 24: ...24 P a g e INSTALLATION ...

Страница 25: ...the user manual Consulter le manuel d utilisation Temperature display Indique l affichage de la température Over Temperature Limit system Thermostat température limite contrôle haute AC Power Repère le courant alternatif I ON O OFF I indique que l interrupteur est en position marche O indique que le commutateur est en position d arrêt Protective earth ground Terre électrique Adjusts UP and DOWN Aj...

Страница 26: ...ed Not disposed of in land fill Indique l appareil doit être recyclé Ne pas jeter dans une décharge Indicates relative humidity Indique humidité relative Indicates water level low Indique faible niveau d eau Indicates CO2 gas Indique gaz CO2 Indicates the incubator heater is active L élément chauffant est la production de chaleur ...

Страница 27: ...During normal operations the Set Temperature display shows the current incubation chamber air temperature accurate to 0 1 C The Up and Down buttons are used to change display modes and then input either a new temperature set point or a calibration adjustment The display blinks continually while in its set point or calibration adjustment modes preceded by an SP for Set Point or C O for calibration ...

Страница 28: ...illuminate whenever CO2 deviations of 1 or greater from the set point take place The yellow LED marked Mute illuminates whenever an audible deviation alarm is being muted See the Muting the Audible CO2 Alarm entry on page 38 for more information Marked CO2 Injecting this green indicator illuminates while the incubator is injecting CO2 into the incubation chamber Injections are accompanied by a cli...

Страница 29: ...lytical feedback loop functions when measuring and controlling the chamber air temperature PID controlled heating pulse intensities and lengths are proportional to the difference between the measured chamber temperature and the current set point The frequency of pulses is derived from the rate of change in that difference The integral function slows the rate of pulses when the temperature nears th...

Страница 30: ...t The pan is then placed on the heated chamber floor Evaporation driven in part by heating raises the relative humidity percentage RH of the chamber A copper token included with the pan helps to significantly slow the growth of microbiological populations in the humidification water supply The incubator must be operated humidified in order to achieve its stated temperature uniformity specification...

Страница 31: ...t in a new workspace environment 1 2 Attach the power cord that came with the unit to the power inlet receptacle on the side of the incubator Plug the power cord into the workspace electrical supply outlet 3 Turn on the incubator The Temperature and CO2 displays will illuminate 4 Humidify the Incubator page 33 5 Set the Temperature Set Point to your incubation application temperature See page 34 6...

Страница 32: ...ak to achieve a stable temperature The manufacturer recommends allowing the unit to run overnight 9 Set the Over Temperature Limit See page 39 The incubator must be heated and stable at your application temperature prior to performing this procedure 10 Place your samples in the incubation chamber See Loading the Incubator page 40 ...

Страница 33: ...al populations in humidification water supply Never use deionized water to humidify the incubator The pan must be placed on the chamber floor o The floor is heated and helps evaporation to raise the humidity level to 90 95 relative humidity Regularly clean and disinfect the pan Refill as needed and change the water in the pan at least once per week Use of chemical disinfectants added to the pan ma...

Страница 34: ...tivity on the arrow keys saving the last shown set point value 3 Set the Temperature Set Point New Set Point Adjust Note Adjust the set point to its lowest setting OFF to turn off heating in the chamber 4 Wait for 5 seconds after entering the Set Point Set Temperature C The display will stop flashing The set point is now saved in the controller See the Set the OTL procedure on page 39 for how to s...

Страница 35: ... for the duration of the current temperature deviation The visual alarm indicator will remain illuminated Any new deviation of 1 C or greater will reactivate the audible alarm AUTOMATIC DOOR CUTOFF Gas heating and air circulation pause Whenever the incubation chamber door is opened the incubator automatically stops the flow of CO2 into the chamber depowers the heater element and ceases operation o...

Страница 36: ...or CO2 in Port Never exceed 20 psi Dual source Set one cylinder regulator to 20 psi and the other regulator to 15 psi o The incubator will draw from and empty the 20 psi cylinder then draw from the 15 psi cylinder o Never exceed 20 psi Building Supply System Initiate a flow to supply15 to 20 psi of CO2 to the incubator either of the Gas In ports PSI Megapascals Kilopascals Bar 15 20 psi 0 103 0 13...

Страница 37: ... End of Procedure 1 Navigate to the CO2 Set Point Adjustment mode Press and hold either Set CO2 Set Point Adjustment Mode Note The display will automatically exit the adjustment mode after 5 seconds of inactivity on the arrow keys saving the last shown set point value 2 Set the CO2 Set Point Set CO2 New Set Point Adjust Note To turn the CO2 system off set the set point to its lowest setting OFF 3 ...

Страница 38: ...rrow button until the Mute light illuminates The alarm will stay muted for the duration of the current temperature deviation A new deviation of 1 or more will reactivate the audible alarm Low and No Gas Alarms Timeline NO GAS SUPPLY ALARM A second alarm activates if the Low Gas deviation indicator light is illuminated for longer than 20 minutes The letters ngS will appear in the CO2 display indica...

Страница 39: ...pacting the unit temperature stability Check if any powered accessories in the chamber are generating heat If you find no sources of external or internal temperature fluctuations contact Tech Support or your distributor for assistance End of Procedure 1 Set OTL control to its maximum setting if not already set to max 2 Turn the dial counterclockwise until the red Over Temperature Limit Light illum...

Страница 40: ...irculation and a high degree of temperature uniformity Leave 1 inch 25mm between sample containers and the chamber walls This completes the Put the Incubator into Operation procedure ACCESSORY COMPATIBILITY Make sure any accessory equipment used inside the incubation chamber can safely and effectively operate within your selected range of temperature and CO2 levels ...

Страница 41: ... 20 milliamp outputs can be connected to a building management system BMS or other data monitoring and capture system through the use of two 2 jack ports on the left side of the incubator These ports accept standard audio jacks available from most electronics retailers inch 2 pole audio connectors also known as phono jacks Jacks are not included with the incubator Jack Port Outputs Parameter Param...

Страница 42: ...ty in the incubation chamber is already near the dew point barometric fluctuations may push it across the dew point threshold Note Thin air at higher altitudes holds less humidity than the denser air found at or near sea level If excessive condensate has appeared in the incubation chamber dry the chamber interior and check the following Verify that the access port stopper is in place on the inside...

Страница 43: ...er Do not clean or disinfect the ring style chamber HEPA filter Replace the filter if discolored or if you believe it has been contaminated Warning Exercise caution if cleaning the unit with alcohol or flammable cleaners Always allow the unit to cool down to room temperature prior to cleaning and make sure all cleaning agents have evaporated or otherwise been completely removed prior to putting th...

Страница 44: ...o thoroughly disinfect the corners Exercise care to avoid damaging the sensor probes Gas concentrations from evaporating disinfecting agents can inhibit growth or cause metabolic symptoms in microbiological sample populations Make sure that chlorines quaternary ammonias or any other overtly volatile disinfecting agents have been rinsed or otherwise removed from the chamber surfaces prior to placin...

Страница 45: ... of use for more than 24 hours to prevent microbiological contamination such as fungus or mold Depower the incubator Disinfect and clean if required by your laboratory protocol or if the chamber has been exposed to pathogenic microorganisms Use a soft cloth to dry the chamber surfaces Do not place the incubator into storage while the chamber surfaces are damp MAINTAINING ATMOSPHERIC INTEGRITY Peri...

Страница 46: ...t Dismount the ceiling air duct using firm but careful pressure to remove the duct feet from the tops of the shelf standards Remove the duct from the chamber Remove the black plastic cap from the HEPA filter by pulling down on it Remove the old HEPA filter by pulling down It will snap out without difficulty Snap the new HEPA filter into position on the duct It may be necessary to tilt the filter s...

Страница 47: ...ry protocol Humidity Humidity affects temperature uniformity and stability in the incubation chamber The chamber must be humidified for at least 24 hours in order to conduct an accurate calibration A suggested calibration setup 2 Position the sensor probe 2 inches 51mm above the the shelving as close as possible to the geometric center point of the chamber Secure with non stick heat resistant tape...

Страница 48: ...1 Once the incubator temperature has stabilized compare the reference device and incubator temperature display readings If the readings are the same or the difference between the two falls within the acceptable range of your protocol the display is accurately showing the incubation chamber air temperature The Temperature Calibration procedure is now complete OR If a difference falls outside of you...

Страница 49: ...mber temperature value 4 Use the Up or Down arrows to adjust the current display temperature value until it matches the reference device temperature reading Reference Device 5 After matching the display to the reference device wait 5 seconds The temperature display will cease flashing and store the corrected chamber display value The incubator will now begin heating or passively cooling in order t...

Страница 50: ... readings fail to match or fall outside of your protocol range Reference Device 8 If the two readings are not the same and the difference still falls outside the acceptable range of your protocol repeat steps 3 7 up to two 2 more times Three 3 attempts may be required to successfully calibrate units that are more than 2 C out of calibration Reference Device 9 If the temperature readings of the inc...

Страница 51: ...uction protocol or regulatory compliance schedule Always calibrate to the standards and use the calibration setup required by your industry requirements or laboratory protocol Humidity Because humidity impacts CO2 concentration through its influence on temperature stability and uniformity the CO2 display should be calibrated with the chamber humidified A suggested calibration setup 1 Connect the r...

Страница 52: ...centration for at least 30 minutes with no fluctuations of 0 1 or greater Continued next page A Suggested CO2 Calibration 1 Once the incubator CO2 concentration has stabilized compare the reference device and the CO2 display readings If the readings are the same or the difference between the two falls within the acceptable range of your protocol the display is accurately showing the incubation cha...

Страница 53: ...ue 4 Use the Up or Down arrows to adjust the current display CO2 value until it matches the reference device concentration reading Reference Device 5 After matching the display to the reference device wait 5 seconds The gas display will cease flashing and store the corrected chamber display value The incubator will now begin injecting CO2 or allowing the current concentration to decay in order to ...

Страница 54: ...tch or fall outside of your protocol range Reference Device 8 If the two readings are not the same and the difference still falls outside the acceptable range of your protocol repeat steps 3 7 up to two 2 more times Three 3 calibration attempts may be required to successfully calibrate units that are more than 2 out of calibration Reference Device 9 Contact your distributor or technical support fo...

Страница 55: ...well as well as scaling and other mineral deposits When 50 of the anode has eroded away a new anode must be installed Tap water with a hardness of more than 30 parts per million or 1 5 grains per gallon will require assessment of the anode on a yearly basis Dissolved gasses in tap water used in the water jacket should never exceed more than 120 parts per million or 7 grains per gallon The pH of th...

Страница 56: ...56 P a g e USER MAINTENANCE ...

Страница 57: ... factory standard following DIN 12880 respecting the recommended wall clearances of 10 of the height width and depth of the inner chamber All indications are average values typical for units produced in the series We reserve the right to alter technical specifications at all times WEIGHT Shipping Unit 284 lbs 129kgs 254 0 lbs 115 2 kg DIMENSIONS By inches Exterior W D H Interior W D H 26 0 x 25 5 ...

Страница 58: ...Cubic Feet Liters 5 1 144 0 CO2 Range Accuracy Recovery Time 0 20 0 1 Less than 5 minutes TEMPERATURE Range Uniformity Stability Ambient 5ºC to 60ºC 0 2ºC at 37ºC 0 1 C 37 C POWER Model AC Voltage Amperage Frequency SCO5W 110 120 5 0 50 60 Hz ...

Страница 59: ...HEPA FILTER 2800517 Humidification Pan 995 00015 Chamber HEPA Filter Cap 6500606 Leveling Foot 2700512 Ceiling Air Duct Chamber 5121518 Power Cord 115 volt 15 Amp 9ft 5 in 2 86m NEMA 5 15P 1800510 CO2 Gas Tubing Kit with In Line HEPA Filter 9710500 Shelf Slides 5121028 Copper Token Humidification Pan 5800529 Shelf Standards 5170646 Fill Port Stopper Water Jacket 7750513 Shelf 5121777 ...

Страница 60: ...r 503 640 3000 Please have the model number and serial number of the incubator ready as Tech Support will need this information to match your unit to its correct part ACCESSORIES The following accessories are available for the SCO5W CO2 Cylinder Regulator Dual Stage For use with a gas supply cylinder tank Part Number 7150509 Copper Shelf Assembly Three copper shelves Includes six copper shelf slid...

Страница 61: ...61 P a g e PARTS LIST ...

Страница 62: ...P O Box 627 Cornelius OR 97113 USA support sheldonmfg com sheldonmanufacturing com 1 800 322 4897 503 640 3000 FAX 503 640 1366 ...

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