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Sample to Insight

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November 2017

EZ1

®

Advanced XL

User Manual

9001874

QIAGEN GmbH

QIAGEN Strasse 1
40724 Hilden

GERMANY

R2

1108598

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Содержание EZ1 Advanced XL

Страница 1: ...Sample to Insight__ November 2017 EZ1 Advanced XL User Manual 9001874 QIAGEN GmbH QIAGEN Strasse 1 40724 Hilden GERMANY R2 1108598...

Страница 2: ...ety 10 2 3 Environment 11 2 3 1 Operating conditions 11 2 4 Waste disposal 11 2 5 Biological safety 12 2 5 1 Samples 12 2 6 Chemicals 12 2 6 1 Toxic fumes 13 2 7 Mechanical hazards 13 2 8 Heat hazard...

Страница 3: ...Z1 Advanced XL 36 4 3 4 Installing the printer 37 4 3 5 Installing the bar code reader 37 4 4 Installing the PC software 38 4 4 1 System requirements 38 4 4 2 Installing the driver of the USB RS 232 c...

Страница 4: ...tubes 55 5 7 Using the UV lamps 56 5 7 1 Switching on the UV lamps 56 5 7 2 Switching off the UV lamps 58 5 7 3 Lifetime reminder of the UV lamps 58 5 7 4 UV lamps ignition error 58 5 8 Manual operati...

Страница 5: ...lar maintenance procedure 78 6 2 Daily maintenance procedure 81 6 3 Weekly maintenance procedure 82 6 4 Reagents for decontamination 83 7 Troubleshooting 85 7 1 EZ1 Advanced XL door errors 85 7 2 Othe...

Страница 6: ...tions or experience any difficulties regarding the EZ1 Advanced XL instrument or QIAGEN products in general do not hesitate to contact us QIAGEN customers are a major source of information regarding a...

Страница 7: ...and training necessary for transportation installation use maintenance and servicing of the EZ1 Advanced XL Type of task Personnel Training and experience Delivery No special requirements No special...

Страница 8: ...this user manual WARNING The term WARNING is used to inform you about situations that could result in personal injury to other persons Details about these circumstances are given in a box like this on...

Страница 9: ...arranty Perform the maintenance as described in Section 6 QIAGEN charges for repairs that are required due to incorrect maintenance Note Do not place items on top of the EZ1 Advanced XL instrument WAR...

Страница 10: ...to a line power outlet that has a protective conductor earth ground Do not adjust or replace internal parts of the instrument Do not operate the instrument with any covers or parts removed If liquid...

Страница 11: ...f 15 cm 6 in and 30 cm 12 in from the rear and sides of the EZ1 Advanced XL Slits and openings that ensure the ventilation of the instrument must not be covered 2 4 Waste disposal Used consumables suc...

Страница 12: ...ional state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America ACGIH American Conference of Government Industrial Hygienists U...

Страница 13: ...ards The worktable of the EZ1 Advanced XL moves during operation of the instrument Never open the EZ1 Advanced XL door while the EZ1 Advanced XL is operating WARNING Moving parts To avoid contact with...

Страница 14: ...table CAUTION Damage to the instrument Do not slam the door It may harm the filament of the UV lamp WARNING Risk of personal injury The UV lamp consists of a robust glass cylinder It is safely located...

Страница 15: ...hol based disinfectant when worktable components have cooled down CAUTION Damage to the instrument Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the EZ1 Advance...

Страница 16: ...the back of the instrument UV radiation hazard avoid looking directly into UV light Do not expose your skin to UV light Robotic arm Crush hazard the nozzle unit may crush your fingers or hand Near th...

Страница 17: ...ope Type plate on the back of the instrument Legal manufacturer Type plate on the back of the instrument Unique Device Identifier UDI as a 2D bar code in Data Matrix format Type plate on the back of t...

Страница 18: ...nced XL User Manual 11 2017 18 2 11 1 Location of symbols Heat hazard symbol on the heating system Biohazard and heat hazard symbols near the tip rack UV radiation hazard symbol on the back of the ins...

Страница 19: ...tion for decontamination The user inserts an EZ1 Advanced XL Card containing protocol s into the EZ1 Advanced XL After starting worktable setup using the control panel and bar code reader the user loa...

Страница 20: ...nced XL door The EZ1 Advanced XL door can be manually opened by the user in order to gain access to the worktable when no protocol is running A protocol cannot be started until the EZ1 Advanced XL doo...

Страница 21: ...emove incorrectly entered values during data entry SHIFT Up Dn Used to enter the installation date The other keys of the control panel are for service personnel only Vacuum fluorescent display VFD The...

Страница 22: ...not to expose the EZ1 Advanced XL card to electric shock water or dirt EZ1 Advanced XL Card inserted into EZ1 Advanced XL Card slot 3 1 4 Status LEDs There are 2 light emitting diodes LEDs at the fro...

Страница 23: ...holding the labware samples and reagent cartridges that are required for the protocol run The worktable also contains a heating system for controlling the temperature of samples The EZ1 Advanced XL wo...

Страница 24: ...ped made of polypropylene supplied by Sarstedt cat no 72 693 and provided in EZ1 Kits Tip rack Both tip holders and filter tips are made of polypropylene and are provided in EZ1 Kits The filter tips h...

Страница 25: ...rtridges are made of polypropylene contain prefilled reagents and are available from QIAGEN Reagent cartridges A reagent cartridge consists of 10 reagent wells and 2 heating positions One heating posi...

Страница 26: ...pumps operate simultaneously to allow aspiration or dispensing of small volumes of liquid 50 1000 l via the filter tips The pipettor head also contains a magnet whose distance from the attached filte...

Страница 27: ...The EZ1 Advanced XL door will be locked during a UV light cycle in order to protect the operator from UV radiation The EZ1 Advanced XL will provide a warning when the lifetime of the lamp is going to...

Страница 28: ...l 11 2017 28 UV lamps and reflector case Location of UV lamps under the EZ1 Advanced XL door CAUTION Damage to the instrument Be careful not to damage the cover of the UV lamps when loading and unload...

Страница 29: ...vanced XL The EZ1 Advanced XL must be placed near to a properly grounded earthed AC power outlet The power line to the instrument should be voltage regulated and surge protected 4 2 AC power connectio...

Страница 30: ...0 120 V A 6 3 A 250 V time delay fuse compatible with 100 120 V power supplies 220 240 V A 3 15 A 250 V time delay fuse compatible with 200 240 V power supplies Before connecting the instrument to the...

Страница 31: ...SB RS 232 adapter Cartridge rack Tip rack Tray O ring pack of 14 and silicon grease Fuses 1 each 6 3 A and 3 15 A LED Type Handy Bar code reader CSA approved EZ1 Advanced XL User Manual this user manu...

Страница 32: ...Pull out the 4 corner shock absorbers at the top and remove the outer cardboard box by pulling it up 6 Take out the wrapped EZ1 Advanced XL and remove the aluminum strap Outer top cardboard Disposabl...

Страница 33: ...x 8 Remove all plastic sheets Note Be careful not to damage the surface of the EZ1 Advanced XL after removing the plastic sheets Note It is recommended to save the original packaging material for late...

Страница 34: ...xes Before using the instrument these protectors must be removed 1 Remove the silica gel bags 2 Push the pipettor head upward 3 Unscrew the knurled knob located inside the instrument at the front This...

Страница 35: ...eled PC Printer at the rear of the instrument Section 3 1 5 page 22 Make sure that the connector is secured with the 2 screws provided Note If your PC does not have a RS 232 port the instrument can be...

Страница 36: ...move the cursor left from YYYY to MM to DD 4 Press the up arrow or down arrow to increase or decrease the value of the selected field 5 After setting the date store the setting by pressing ENT If you...

Страница 37: ...a 25 pin connector on the other end 7 Connect the printer s data cable to the EZ1 Advanced XL The connector is located at the rear of the instrument labeled PC Printer Section 3 1 5 page 22 Make sure...

Страница 38: ...d to receive report files from EZ1 Advanced XL and to store data on the PC hard disk Important To install the software you must have administrator rights Otherwise error messages will appear and the s...

Страница 39: ...RS 232 port use the USB RS 232 converter First install the driver by inserting the installation CD and following the instructions given The following installation wizard window appears 2 Click Next M...

Страница 40: ...ailable COM ports 6 Connect the converter to an open USB port on the PC The device manager should then automatically update the COM port list Look for the COM port named USB to Serial Bridge 7 Right c...

Страница 41: ...automatically start setup exe The installation wizard will guide you through the installation process Note To open the user interface of the EZ1 Advanced Communicator double click on the EZ1 icon in t...

Страница 42: ...r this application only the QIAGEN PC can be used This PC is equipped with a 1 x 4 Port Serial board To set up your PC follow the directions given below 1 Connect the large connector of the fourfold m...

Страница 43: ...ick the Start button on your PC and select Settings Control Panel 5 Double click System 6 Click the Hardware tab and select the Device Manager button 7 Expand Ports to show all available COM ports The...

Страница 44: ...follow your organization s instructions and guidelines In addition follow the steps below 1 Decontaminate the instrument 2 Pack the EZ1 Advanced XL by following the steps of the unpacking procedure de...

Страница 45: ...lead you through the setup of the worktable by displaying messages in the display For details refer to the handbook supplied with the EZ1 kit you are using If you want the EZ1 Advanced XL to generate...

Страница 46: ...Advanced XL card 1 Open the cover of the EZ1 Advanced XL card slot 2 Insert the EZ1 Advanced XL card into the EZ1 Advanced XL card slot 3 Orientate the card so that the picture faces to the left and t...

Страница 47: ...EZ1 Advanced XL Section 5 3 1 page 48 Note Do not remove the EZ1 Advanced XL Card while the machine is switched on 5 2 2 Removing the EZ1 Advanced XL card 1 Switch off the EZ1 Advanced XL Section 5 3...

Страница 48: ...he rear power switch 3 The display shows the following screen for a few seconds This text indicates that the EZ1 Advanced XL is initializing All modules move to their home positions After initializati...

Страница 49: ...ntil the upper end slides into position The door will be automatically held open 5 4 2 Closing the EZ1 Advanced XL door 1 Press the door down to disengage the door The door is secured in the upper pos...

Страница 50: ...ting a protocol run After you have inserted the EZ1 Advanced XL card and switched on the EZ1 Advanced XL start a protocol as follows 1 Press START on the control panel The protocol will lead you throu...

Страница 51: ...uested during setup and send it either to the PC or the printer 8 At the end of the protocol run you may optionally choose to perform a UV decontamination run For details refer to the handbook supplie...

Страница 52: ...nceling a protocol press 2 to display Manual in the display Press 2 again to return the tips to the tip rack and to return the modules to their home positions 5 6 Setting up the worktable Note For spe...

Страница 53: ...reagent cartridges Note Do not remove the foil from the reagent cartridges 1 Invert the reagent cartridges several times to mix the magnetic particles Use the same number of reagent cartridges as the...

Страница 54: ...e arrow as shown below until you feel resistance 5 Press down the cartridges until they click into place Note If fewer than 14 cartridges are used they may be loaded in any order on the rack However w...

Страница 55: ...ubes filter tips tip holders sample tubes and any other equipment or reagents as described in the messages shown in the display and described in the relevant EZ1 kit handbook Make sure that the elutio...

Страница 56: ...nually by switching on the UV lamp as described below Note UV decontamination helps to reduce possible pathogen contamination of the EZ1 Advanced XL worktable surfaces The efficiency of inactivation h...

Страница 57: ...er an invalid decontamination time 20 minutes or 60 minutes the following screen will appear UV decontamination time must be between 20 60 min ESC Back 5 After entering a valid time press START to swi...

Страница 58: ...ps is limited to 1500 cycles The instrument will remind you when the UV lamps will expire by displaying the following screen UV Lamp Reminder UV lamp expires soon UV runs left CC ENT Continue Beginnin...

Страница 59: ...manual operation screen to choose the home axis operation The following screen appears MANUAL OPERATION Home axis 0 ALL 1 Y 2 Z 3 P 4 M ESC Back Press 0 to 4 to select the ALL Y Z P or M functions AL...

Страница 60: ...een 5 8 3 Manual operation clean Use the clean operation to get access to the piercing unit for cleaning The EZ1 Advanced XL door must initially be closed Press 3 in the manual operation screen to cho...

Страница 61: ...While the operation is in progress the following screen appears MANUAL OPERATION Resend report file Executing Alternatively if there are no report files temporarily stored on the EZ1 Advanced XL the...

Страница 62: ...choose the Axis operation TEST 1 Axis 2 Temp 3 Serial 4 Version ESC Back The following test procedures will be carried out by the EZ1 Advanced XL Move modules to their home positions Pick up tip 2 an...

Страница 63: ...nual 11 2017 63 Proc positions tested by the EZ1 Advanced XL test procedure First 3 rows shown of 14 in total Proc 12 Proc 11 Proc 9 Proc 8 Proc 7 Proc 6 Proc 5 Proc 4 Proc 3 Proc 2 Proc 1 Tip 1 Eluat...

Страница 64: ...down arrow to increase or decrease the temperature The upper limit is 99 C Press START to start the heating process The following screen appears TEST TEMPERATURE Temp SS S C Actual RR R C S ESC Back...

Страница 65: ...Section 5 10 3 page 68 PC Press START to send a test string to the PC The following screen appears TEST PC Printer Target PC Result PASSED ESC Back If the transmission is successful the result will s...

Страница 66: ...overwritten A beep sounds after a successful scan of the bar code The following screen appears showing up to 25 digits TEST Barcode Result BBBBBBBBBBBB BBBBBBBBBBBBB ESC Back B indicates the individua...

Страница 67: ...he day month and year Press SHIFT down arrow to move the cursor right from DD day to MM month to YYYY year Press SHIFT up arrow to move the cursor left from YYYY to MM to DD Press the up arrow or down...

Страница 68: ...evice for the report file Press 3 in the system setup menu to change the setting of the serial port SYSTEM SETUP 1 Date 2 Time 3 SerialPort 4 PM ESC Back The following screen appears SETUP SERIAL PORT...

Страница 69: ...d a bar code press the button located on the inner side of the handle of the bar code reader A red light appears Hold the bar code reader in front of the bar code at a distance of approximately 20 mm...

Страница 70: ...e report file is shown below Example printout of a report file Note If the printer is turned on before switching on the EZ1 Advanced XL some additional characters will be printed before the report To...

Страница 71: ...powered stored permanently on the EZ1 Advanced XL Weekly maintenance done on Feb 10 2017 When you accept the weekly maintenance reminder the date is stored and indicated here Yearly maintenance done o...

Страница 72: ...Status run o k The status of the run can show o k not o k aborted Error code 21 See Section 7 2 for error codes Sample input volume l 300 Sample input volume in microliters depending on the protocol...

Страница 73: ...port will be erased 1 Next ESC Abort Press 1 to delete the oldest report file and continue the protocol run Alternatively press ESC to cancel the protocol run 5 14 Using the EZ1 Advanced Communicator...

Страница 74: ...ime in format HH MM SS File extension csv comma separated value The date and time are when the report file was received by the PC The Status Message panel displays the name of the last report file tha...

Страница 75: ...as shown above baud rate of 9600 bits s 8 data bits parity none 1 stop bit flow control none Select About in the main window to show the version of the EZ1 Advanced Communicator software Click the Ch...

Страница 76: ...ng multiple EZ1 Advanced XL instruments Up to 4 EZ1 Advanced XL instruments can send report files to a single PC For this configuration only the QIAGEN PC can be used For information about installing...

Страница 77: ...semiannually depending on how the reminder was set see Section 5 10 4 page 68 QIAGEN Instrument Service Specialists only Maintenance reminders The EZ1 Advanced XL has a built in clock and calendar de...

Страница 78: ...for response to a breakdown is 5 days Application development software upgrades worktable accessories disposable items and replacement of spare parts such as syringes tubing and pipet tips are not in...

Страница 79: ...disposal of wastes must be in accordance with all national state and local health and safety regulations and laws If working with potentially infectious materials such as human blood serum or plasma...

Страница 80: ...and wipe the piercing unit using a soft tissue moistened with 70 ethanol The piercing unit is sharp Two pairs of gloves are recommended 7 Wipe the piercing unit with a soft tissue moistened with dist...

Страница 81: ...ue door using a soft tissue moistened with 70 ethanol 6 2 Daily maintenance procedure CAUTION Hazardous materials and infectious agents The waste contains samples and reagents This waste may contain t...

Страница 82: ...nce procedure To maintain good contact between tip adapters and filter tips and to prevent liquid leaking from the tips grease the O rings of the tip adapters every week 1 Apply a small amount of sili...

Страница 83: ...f the EZ1 Advanced XL 6 4 Reagents for decontamination The following disinfectants and detergents are compatible with metallic surfaces and removable components of the EZ1 Advanced XL system Use accor...

Страница 84: ...fectants different from those recommended ensure that their compositions are similar to those described above A suitable alternative to Mikrozid Liquid is Incidin Liquid EcoLab www ecolab com A suitab...

Страница 85: ...the locking mechanism is defective the following error screen will be displayed ERROR Can not lock Close the door and retry ESC Retry Press ESC to retry If the error persists contact QIAGEN Technical...

Страница 86: ...inue a protocol run that has been interrupted due to an error 7 3 Error codes Error code Description 10 Home sensor not activated during an operation other than a protocol 11 End limit sensor not acti...

Страница 87: ...t Y axis does not respond during a protocol run 24 Door open when starting an operation 25 Bottom sensor of pipettor head Z axis activated during an operation 26 Communication error between heating bl...

Страница 88: ...nced XL and is inserted into the instrument EZ1 Advanced XL card slot A slot at the front of the EZ1 Advanced XL that accepts an EZ1 Advanced XL card EZ1 Advanced XL door The main door at the front of...

Страница 89: ...ts Tip adapter One of 14 metal probes installed on the pipettor head During operation of the EZ1 Advanced XL the tip adapters pick up filter tips from the worktable Tip holder A polypropylene tube tha...

Страница 90: ...50 V time delay fuse for 200 240 V AC WARNING Electrical hazard Never install a fuse different from that specified in the user manual Overvoltage category II Air temperature 15 30 C 59 86 F Relative h...

Страница 91: ...rticles Desktop instrument Protocols stored on EZ1 Advanced XL Cards To be used only with QIAGEN EZ1 kits Aspirates and dispenses 14 samples or reagents simultaneously using a 14 channel pipettor head...

Страница 92: ...cartridges and has a temperature range of between ambient temperature and 95 C 203 F Heating block accuracy at 60 C is 2 C Filter tips Attach to the tip adapters of the pipettor head to allow liquid a...

Страница 93: ...ime of disposal helps to conserve natural resources and ensures that the product is recycled in a manner that protects human health and the environment Recycling can be provided by QIAGEN upon request...

Страница 94: ...with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated...

Страница 95: ...meets all applicable requirements of the following European Directives Low Voltage Directive LVD Electromagnetic Compatibility Directive EMC In vitro diagnostic medical devices IVD 2014 35 EU 2014 30...

Страница 96: ...in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices interfacing devices and associated software will be warr...

Страница 97: ...daily 81 decontamination 83 reminder 36 77 schedule 77 weekly 82 Manual operation clean 60 home axis 59 resend 61 return tip 60 Mechanical data 91 Multiple instruments 42 76 Operating conditions 90 Pi...

Страница 98: ...ce 6 Test axis 62 heating block 64 serial port 64 version 66 Tip adapters 26 Tip holders 24 Tip rack 23 Transport and removal 44 Transportation conditions 90 Tray 26 52 Troubleshooting door errors 85...

Страница 99: ...ht EZ1 QIAGEN Group DECON QUAT Veltek Associates Inc Gigasept Lysetol Mikrozid Parmetol Sch lke Mayr GmbH IBM IBM Corporation Microsoft Windows Windows Vista Microsoft Corporation Sarstedt Sarstedt AG...

Страница 100: ...EZ1 Advanced XL User Manual 11 2017 100 Ordering www qiagen com shop Technical Support support qiagen com Website www qiagen com...

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