Rotor-Gene Q MDx CE User Manual 02/2022
109
Normalization options:
A variety of options are available to optimize raw data normalization:
•
Dynamic Tube
(dynamic tube normalization)
•
Slope Correct
(noise slope correction)
•
Ignore First x cycles
(correction for noise in initial cycles)
•
Takeoff point adjustment
For more details, see page 91.
Discrimination Threshold
:
Enter values in these text boxes to position the discrimination threshold. All curves
passing this threshold are considered to be genotyping samples. Click on the icon to
the right of each text box, then drag the threshold on the graph to set these values
visually.
Genotypes:
This opens the
Genotyping
window, which is used to define which genotype is
detected in each channel. This window enables genotypes to be assigned to
channels for allelic discrimination analysis.
In the example below, a sample is heterozygous if readings in channels Cycling
A.Green and Cycling A.Yellow cross the threshold.
Allelic analysis templates:
Allelic analysis templates enable export of normalization, threshold, and genotype
settings into a single *
.alt
file. This file can be imported and reapplied in other
experiments. See Section 7.1 for more details.
6.6.8
Scatter graph analysis
Scatter graph analysis enables genotyping based on the relative expression of amplification plots
across 2 channels. Unlike allelic discrimination, genotype is decided based on regions defined
from the scatter graph rather than from a single threshold. To perform this analysis, select
Other
and then
Scatter Graph Analysis
in the
Analysis
window.
