Rotor-Gene Q MDx CE User Manual 02/2022
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Autoscale
:
Autoscale
attempts to fit the scale to the maximum and minimum readings of the data.
Default Scale
:
Default Scale
resets the scale to display from 0 to 100 fluorescence units.
Spanner/wrench icon:
See Section 7.5 for more information.
Options
:
This displays the drop-down menu shown above, which provides options for
transformation of the raw data.
Normalise to…:
This enables normalization of amplification data to data from a passive
reference dye, such as ROX, acquired in another channel.
Crop start cycles
:
This creates a new channel data set in which some start cycles have been removed.
This is useful if large jumps are observed in the initial cycles, which can occur when
using certain chemistries.
Crop end cycles
:
This creates a new channel data set in which some end cycles have been removed.
Page 1
:
This indicates the page that is currently selected to display the raw data plots. The
Edit Sample
window allows the creation of multiple sample definitions. For example,
data can be viewed with varying line thickness, sample definitions, and other display
options. This is particularly useful if relative quantitation is performed in a single
channel, because the user can easily switch the view between the gene of interest
and housekeeper samples by defining 2 sample pages.
6.4
Toggling samples
At the right-hand side of the main window is a toggler, which includes a sample legend. This consists
of colored cells, each of which corresponds to a sample in the display. The toggler is used to control
which samples can be seen in the display. Samples with a brightly colored cell are displayed while
samples with a faded cell are not displayed. Samples can be switched on or off by clicking on the
cell or by dragging the mouse pointer across several cells at time. The
Bank On
and
Bank Off
buttons hide or display, respectively, all samples currently visible in the list. The scroll bar can be
used to display the next group of samples.
Note
: The number of displayed samples is dynamic, and depends on the space available in the
window.
