Rotor-Gene Q MDx CE User Manual 02/2022
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analysis of DNA methylation status. It can also be used to quantitatively detect a small proportion
of variant DNA in a background of wild-type sequence at sensitivities approaching 5%. This can
be used, for example, to study somatically acquired mutations or changes in the methylation state
of CpG islands.
HRM on the Rotor-Gene Q MDx facilitates multiple applications, including:
Identification of candidate predisposition genes
Association studies (comparing cases and controls, genotype to phenotype)
Determination of allele prevalence within a population or subgroup
SNP screening and validation
Screening for loss of heterozygosity
DNA fingerprinting
Characterization of haplotype blocks
DNA methylation analysis
DNA mapping
Species identification
Mutation discovery
Determining the ratio of somatic acquired mutations
HLA typing
HRM is easier and more cost-effective than probe-based genotyping assays and, unlike conventional
methods, it is a closed-tube system that prevents contamination with PCR products. Results are
comparable to conventional methods such as SSCP, DHPLC, RFLP, and DNA sequencing.
10.1
Instrumentation
The Rotor-Gene Q MDx provides the following demanding real-time and thermo-optical capabilities
required for HRM.
High-intensity illumination
Highly sensitive optical detection
Fast data acquisition
Finely controlled sample temperature
Minimal sample-to-sample thermal and optical variation
