Rotor-Gene Q MDx CE User Manual 02/2022
114
Click
OK
. The
Edit Samples
window appears, allowing positive and negative controls to be defined.
To define a sample as a positive or negative control, click on the sample type cell, then select the
relevant control type from the drop-down menu.
Note
: The controls must be toggled “on”, using the toggler at the right-hand side of the main
window, to perform the analysis.
This screen functions in the same way as the
Edit Samples
window (Section “Sample setup”).
Normalization
Normalization of EndPoint analysis data scales all signal levels to within the range of 0–100%. At
least one positive and one negative control must be selected, or more if analyzing multiple channels
and the standards are not multiplexed. More than one positive and one negative control should be
run if there is a risk that a positive control may not amplify.
1.
For each channel, all the positive controls are analyzed, and the one with the highest
fluorescence is set to be 100%. This means that if duplicate controls are run, a positive control
can fail without affecting the run.
2.
All the negative controls are analyzed, and the one with the lowest fluorescence level is set to
be 0%.
3.
The raw fluorescence values of the remaining samples are scaled relative to the highest
positive control and the lowest negative control.
