Rotor-Gene Q MDx CE User Manual 02/2022
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dragging with the left mouse button, and the values are displayed in a table on the right of the
screen.
In this screenshot, the concentrations of several samples are analyzed.
Important
: The
AutoStat
feature is context-aware. This means that, where possible, it only generates
information that is useful.
For example:
It is not possible to obtain a 95% confidence interval from a set of selected calculated
concentrations because the regression model must also be taken into account.
The “Orders of Magnitude” standard deviation is reported for calculated concentrations rather than
an absolute value. This is a percentage variation. For example, a value of 1.07537 represents a
7.54% variation (278,974 – 322,611) = (300,000/1.07537 – 00,000 * 1.07537). Reporting an
absolute value does not make sense for a standard curve. The value could be reported at the lowest
concentration to create a perceived low error (± 3 copies) or at the high concentration (± 3,000,000
copies). For this reason, the “Orders of Magnitude” standard deviation is reported.
For calculated concentrations, the geometric mean is used instead of the arithmetic mean. This
accounts for the exponential nature of real-time PCR. For example, in the case of twofold
dilutions with 1, 2, 8, and 16 copies, the average should be 4 copies, because it is the
middle of the dilution series. However the arithmetic mean is 6.75. The geometric mean is
(1 * 2 * 8 * 16)^(1/4) = 4 copies.
Dynamic tube normalization
The
Dynamic Tube
option is selected by default and is used to determine the average background
of each sample just before amplification commences.
