21
BEFORE COMMENCING OPERATION
Initial cleaning method
Before using the incubator for the first time, clean dirt (tape residue, smear, etc.) from the
chamber and the inner attachments thoroughly. To keep the chamber clean is essential to
get the proper performance out of the incubator. When the chamber is contaminated or
when cleaning the chamber prior to starting a culture, it is possible to perform dry heat
sterilisation (refer to pages 62~66).
Use the following steps to clean the incubator properly.
1.
Remove the inner attachments, referring to “Removing inner attachments” on page 22.
2.
Clean the removed inner attachments, the chamber inside walls and the inner door gaskets with a cloth
or sponge soaked in neutral detergent, diluted by 5 % or less. (Undiluted detergent can damage the plastic
components. For the dilution, refer to the instruction of the detergent.) (Fig. 1)
CAUTION
Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause
discoloration, corrosion, or rusting.
Be careful to keep the diluted detergent or water out of the temperature sensor, the CO
2
gas injection port,
the inner sample air access port, the fan motor shaft bearing, and the inner sample air outlet (Fig.2 ).
Also, do not wash the temperature sensor and the UV lamp using detergent. Doing so may cause failure.
(Fig. 2)
3.
Soak a gauze or unwoven cloth in distilled water and wring it tightly, and then wipe off the residual
detergent thoroughly.
4.
Wash the silicon caps (2 pcs) for the access port and the fan using the above mentioned detergent and
rinse them with distilled water, and then autoclave them for sterilisation (121
o
C, 20 minutes).
5.
Wipe up the inside walls and the inner attachments like trays thoroughly with a cloth or unwoven cloth
soaked in alcohol for disinfection. Be careful not to leave any residue alcohol.
Note:
Keep fire away when using alcohol for disinfection.
6.
Reinstall the inner attachments correctly and securely, referring to “Installing inner attachments” on page 24.
Fig. 1
Fig. 2
Silicon
cap
Temp. sensor
UV lamp
Inner door gasket