PerkinElmer OPERETTA, Техническое руководство

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5.3 Specifications
5.3.1 Objective Lenses
Objective Numerical
aperture Working
distance
[mm] Field of
view [µm
2
]
Depth of
focus
[µm] Optical
resolution
xy [µm]
2x long WD 0.08 6.2 6754 × 5086 145.5 3.75
10x long WD 0.3 10 1351 × 1017 10.3 1
10x high NA 0.4 3.1 1351 × 1017 5.8 0.75
20x long WD 0.45 6.6-7.8 675 × 509 4.6 0.67
20x high NA 0.75 0.6 675 × 509 1.7 0.4
40x long WD 0.6 2.7-4.0 338 × 254 2.6 0.5
40x high NA 0.95 0.18 338 × 254 1.0 0.32
60x long WD 0.7 1.5-2.2 225 × 170 1.9 0.43
60x high NA 0.9 0.2 225 × 170 1.1 0.33
100x long WD 0.85 1.2-1.9 135 × 102 1.3 0.35
5.3.2 Sample Carrier
Recommended sample carriers for the Operetta:
Plate type Bottom
material
supports
adherence of
cells Plate bottom
allows use of
high NA
objectives Suitable for
the following
objectives
ViewPlate-96 F TC (black, with
lid, TC, sterile, individually
pouched) bottom thickness of
0.8 mm yes no 2x long WD
10x long WD
10x high NA*
20x long WD
40x long WD*
ViewPlate-384 F TC (black, with
lid, TC, sterile) yes no 2x long WD
10x long WD
10x high NA*
20x long WD
40x long WD*
5 Technical Data
Operetta Technical Manual 59