PerkinElmer OPERETTA, Техническое руководство

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3.1.2 Correction Collar
Some objective lenses for the Operetta (see table on the next page) have an integrated
correction collar. It can be rotated and allows the correction of spherical aberrations
mainly caused by differences in plate bottom thickness. Underneath the colored ring of
the objective you can find a small white line which should be set directly on the value
of the plate bottom thickness.
The Harmony software will remind you to adjust the correction collar if you select a
different plate type or objective during experiment definition. The required plate bottom
thickness of the current plate type will also be stated.
Also please note, that while the message box “Please adjust the correction collar” is
displayed the table is positioned so as to give easy access to the objective.
The objectives have a scale engraved, which you can see on the photograph below.
The unit of values of the scale is typically [mm]. There are different scales used
depending on the range of compatible plate bottom thicknesses. E.g. the scale of the
20x WD lens ranges from 0 to 2 mm, often the value of 0.17 is also depicted, as this is
a very common thickness of cover slips used in microscopy.
Next to the scale you can find a small singular mark, which indicates the current
setting of the correction collar.
Below you find some example settings:
l
0.17 mm
l
0.50 mm
l
0.75 mm
3 Retooling and Maintenance
36 Operetta Technical Manual