PerkinElmer OPERETTA, Техническое руководство

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2.1 Introduction
This chapter describes the most relevant hardware components of the Operetta
®
.
Please note that your specific instrument may not be equipped with all features or
optional upgrades described in this manual (dependent on your selected
instrumentation)!
For a description of the Operetta control software (Harmony
®
) please refer to the
Harmony Help or to the separate Operation Manual.
The Operetta is an entry level to medium throughput microplate reader for high content
screening (HCS). It can acquire, analyze and manage fluorescence and brightfield
images. A set of ready-made image analysis solutions is included consisting of
building blocks which can also be assembled to new analysis solutions.
The Operetta Application Guide helps you getting started with HCS. It describes the
essentials of setting up an HCS assay and how to use the Operetta and image
analysis software for the most common applications.
2.1.1 Instrument Features
l
High content imaging reader
l
Switchable between non-confocal and confocal
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High power Xenon lamp (300W) providing entire visible spectrum
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Peltier cooled high resolution 14 bit CCD camera
l
Brightfield excitation
l
4 or 8 excitation filters, up to 8 emission filters choosable from 17
l
Up to 4 automatically exchangeable objective lenses for different fields of view /
resolutions (2x to 100x objectives, high NA or long WD)
l
Fast laser based auto focus
l
Focal plane thickness 1.5 – 145 µm
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Simple experiment set-up
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Calibration free
l
Harmony software for fully automated image acquisition, online evaluation and
data management
l
Supports various sample carrier formats, e.g. 96 and 386 well plates
l
Plug and play applications with ready-made image analysis solutions as a
starting point
2 Hardware Description
20 Operetta Technical Manual