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Moxi GO™ User Guide
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Introduction
The Moxi GO™ from Orflo Technologies is a benchtop instrument that utilizes a combination of
a 532 nm laser (MXG001 model) or 488nm laser (MXG002 model), one photomultiplier tube,
Coulter Principle-based cell measurements, and on-board software to provide easy-to- run
applications and data analysis. The 532nm system comes with a built-in 561nm/LP PMT
emission filter, ideal for phycoerythrin (PE) conjugated antibodies, propidium iodide (PI) viability
staining. The 488nm system comes with both a 525/45nm PMT emission filter (ideal for FITC,
GFP and other similar dyes) and a 561nm/LP filter (ideal for PE and PI). The filters are
swappable depending on the sample to be run. The instrument relies on disposable cassettes
for sample handling, which alleviates the need for flow cell cleaning and fluidics maintenance
and the instrument is small enough to be portable between the lab bench and the hood.
The Moxi GO™ enables highly accurate cell counts and quantitative assessments of cell
viability, apoptosis, other labeled antibody markers along with multiplexed bead-based assays
for protein and cellular analysis.
Data acquisition occurs within approximately 10 seconds per
test. Test results are displayed in the form of histograms and scatter plots, and quantitatively
show cell number, cell percentage(s) gated, and mean fluorescent intensity value(s) in a
graphical display. The instrument can store up to 16 Mb of data and the data may be
downloaded to a PC or Mac via USB connectivity.
IMPORTANT NOTE :
The Moxi GO™ is intended for research use only. It is not intended for use in medical diagnostic
or therapeutic procedures, and it has not been approved or cleared by the Unites States Food
and Drug Administration for such uses.
About the User Guide
The Moxi GO™ User Guide provides detailed information for operating, maintaining, and
troubleshooting the Moxi GO™, including the system, the Cassettes, and application workflows.
This includes information on how to analyze test results using the onboard analysis features of
the software.
The user guide does not include instructions for analyzing the provided/output flow cytometry
standard (FCS, version 3.1) files offline in third party software.
Similarly, the user guide does not include detailed protocols for preparing samples prior to
running them on the instrument. The results of the assays are dependent upon the proper use
of reagents and the instrument.